GDNF stimulates the proliferation of cultured mouse immature Sertoli cells via its receptor subunit NCAM and ERK1/2 signaling pathway

<p>Abstract</p> <p>Background</p> <p>The proliferation and final density of Sertoli cells in the testis are regulated by hormones and local factors. Glial cell line-derived neurotrophic factor (GDNF), a distantly related member of the transforming growth factor-β superfamily, and its receptor subunits GDNF family receptor alpha 1 (GFRα1), RET tyrosine kinase, and neural cell adhesion molecule (NCAM) have been reported to be expressed in the testis and involved in the regulation of proliferation of immature Sertoli cells (ISCs). However, the expression patterns of these receptor subunits and the downstream signaling pathways have not been addressed in ISCs.</p> <p>Results</p> <p>In the present study, we have reported that the proliferation of cultured ISCs was significantly enhanced by GDNF. The receptor subunits GFRα1 and NCAM but not RET were expressed in ISCs, and the stimulatory effect of GDNF on the proliferation of ISCs was significantly reduced by anti-NCAM antibody blocking or siRNA that specifically targets NCAM mRNA. Additionally, the ERK1/2 inhibitor, PD98059, completely abolished the mitogenic effect of GDNF on ISCs.</p> <p>Conclusions</p> <p>GDNF stimulates the proliferation of ISCs via its receptor subunit NCAM and the consequent activation of the ERK1/2 signaling pathway.</p

A multiqueue interlacing peak scheduling method based on tasks’ classification in cloud computing

In cloud computing, resources are dynamic, and the demands placed on the resources allocated to a particular task are diverse. These factors could lead to load imbalances, which affect scheduling efficiency and resource utilization. A scheduling method called interlacing peak is proposed. First, the resource load information, such as CPU, I/O, and memory usage, is periodically collected and updated, and the task information regarding CPU, I/O, and memory is collected. Second, resources are sorted into three queues according to the loads of the CPU, I/O, and memory: CPU intensive, I/O intensive, and memory intensive, according to their demands for resources. Finally, once the tasks have been scheduled, they need to interlace the resource load peak. Some types of tasks need to be matched with the resources whose loads correspond to a lighter types of tasks. In other words, CPU-intensive tasks should be matched with resources with low CPU utilization; I/O-intensive tasks should be matched with resources with shorter I/O wait times; and memory-intensive tasks should be matched with resources that have low memory usage. The effectiveness of this method is proved from the theoretical point of view. It has also been proven to be less complex in regard to time and place. Four experiments were designed to verify the performance of this method. Experiments leverage four metrics: 1) average response time; 2) load balancing; 3) deadline violation rates; and 4) resource utilization. The experimental results show that this method can balance loads and improve the effects of resource allocation and utilization effectively. This is especially true when resources are limited. In this way, many tasks will compete for the same resources. However, this method shows advantage over other similar standard algorithms

Ensiling Characteristics and Ruminal Degradation of Italian Ryegrass with or without Wilting and Added Cell Wall Degrading Enzymes

The previous experiment (Yu zhu et al.,1999) has shown that the efficacy of added enzymes varied greatly according to the DM content of the material crop. The silage DM did not alter the effects of enzymes on the in vitro digestion of NDF (Yu zhu et al.,1999, Yu zhu et al.,2000). The aim of this experiment was to study the effect of wilting and enzymes on fermentation quality, chemical composition and in situ digestion of Italian ryegrass (Lolium multiflorum Lam.) silage

Effects of Saline and Alkaline Stresses on Growth and Physiological Changes in Oat (Avena sativa L.) Seedlings

Two neutral salts (NaCl and Na2SO4) and alkaline salts (NaHCO3 and Na2CO3) were both mixed in 2:1 ratio, and the effects of saline and alkaline stresses on growth and physiological changes in oat seedlings were explored. The result showed that biomass, water content and chlorophyll content decreased while cell membrane permeability significantly increased under alkaline stress. Saline stress did not have an obvious effect on pH value in tissue fluids of shoot and root, but alkaline stress increased pH value in the root tissue fluid. The contents of Na+, Na+/K+, SO42- increased more, and K+, NO3-, H2PO4- decreased more under alkaline stress, the Cl- content increased obviously under saline stress but had little change under alkaline stress. The increments of proline and organic acid were both greater under alkaline stress, but organic acid content kept the same level under saline stress. Alkaline stress caused more harmful effects on growth and physiological changes in oat seedlings especially broke the pH stability in the root tissue fluid. Physiological adaptive mechanisms of oat seedlings under saline stress and alkaline stress were different, which mainly took the way of accumulating organic acid under alkali stress but accumulating Cl- under saline stress

An integrated natural remanent magnetization acquisition model for the Matuyama-Brunhes reversal recorded by the Chinese loess

Geomagnetic polarity reversal boundaries are key isochronous chronological controls for the long Chinese loess sequences, and further facilitate paleoclimatic correlation between Chinese loess and marine sediments. However, owing to complexity of postdepositional remanent magnetization (pDRM) acquisition processes related to variable dust sedimentary environments on the Chinese Loess Plateau (CLP), there is a long-standing dispute concerning the downward shift of the pDRM recorded in Chinese loess. In this study, after careful stratigraphic correlation of representative climatic tie points and the Matuyama-Brunhes boundaries (MBB) in the Xifeng, Luochuan, and Mangshan loess sections with different pedogenic environments, the downward shift of the pDRM is semiquantitatively estimated and the acquisition model for the loess natural remanent magnetization (NRM) is discussed. The measured MB transition zone has been affected by the surficial mixing layer (SML) and remagnetization. Paleoprecipitation is suggested to be the dominant factor controlling the pDRM acquisition processes. Rainfall-controlled leaching would restrict the efficiency of the characterized remanent magnetization carriers aligning along the ancient geomagnetic field. We conclude that the MBB in the central CLP with moderate paleoprecipitation could be considered as an isochronous chronological control after moderate upward adjustment. A convincing case can then be made to correlate L8/S8 to MIS 18/1

Fluorinated solid electrolyte interphase enables highly reversible solid-state Li metal battery

Partial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.Solid-state electrolytes (SSEs) are receiving great interest because their high mechanical strength and transference number could potentially suppress Li dendrites and their high electrochemical stability allows the use of high-voltage cathodes, which enhances the energy density and safety of batteries. However, the much lower critical current density and easier Li dendrite propagation in SSEs than in nonaqueous liquid electrolytes hindered their possible applications. Herein, we successfully suppressed Li dendrite growth in SSEs by in situ forming an LiF-rich solid electrolyte interphase (SEI) between the SSEs and the Li metal. The LiF-rich SEI successfully suppresses the penetration of Li dendrites into SSEs, while the low electronic conductivity and the intrinsic electrochemical stability of LiF block side reactions between the SSEs and Li. The LiF-rich SEI enhances the room temperature critical current density of Li3PS4 to a record-high value of >2 mA cm−2. Moreover, the Li plating/stripping Coulombic efficiency was escalated from 88% of pristine Li3PS4 to more than 98% for LiF-coated Li3PS4. In situ formation of electronic insulating LiF-rich SEI provides an effective way to prevent Li dendrites in the SSEs, constituting a substantial leap toward the practical applications of next-generation high-energy solid-state Li metal batteries.https://doi.org/10.1126/sciadv.aau924

Identifying the components of the solid–electrolyte interphase in Li-ion batteries

The importance of the solid–electrolyte interphase (SEI) for reversible operation of Li-ion batteries has been well established, but the understanding of its chemistry remains incomplete. The current consensus on the identity of the major organic SEI component is that it consists of lithium ethylene di-carbonate (LEDC), which is thought to have high Li-ion conductivity, but low electronic conductivity (to protect the Li/C electrode). Here, we report on the synthesis and structural and spectroscopic characterizations of authentic LEDC and lithium ethylene mono-carbonate (LEMC). Direct comparisons of the SEI grown on graphite anodes suggest that LEMC, instead of LEDC, is likely to be the major SEI component. Single-crystal X-ray diffraction studies on LEMC and lithium methyl carbonate (LMC) reveal unusual layered structures and Li+ coordination environments. LEMC has Li+ conductivities of >1 × 10−6 S cm−1, while LEDC is almost an ionic insulator. The complex interconversions and equilibria of LMC, LEMC and LEDC in dimethyl sulfoxide solutions are also investigated