3,5-Dimethyl-1H-pyrazole–2-hy­droxy-5-(phenyl­diazen­yl)benzoic acid (1/1)

There are two independent 3,5-dimethyl­pyrazole and two independent 2-hy­droxy-5-(phenyl­diazen­yl)benzoic acid mol­ecules [in which intra­molecular O—H⋯O bonds form S(6) graph-set motifs] in the asymmetric unit of the title compound, C5H8N2·C13H10N2O3. In the crystal, the components are linked by inter­molecular O—H⋯O, O—H⋯N and N—H⋯O hydrogen bonds, forming four-component clusters. Further stabilization is provided by weak C—H⋯π inter­actions

Up-regulation of microRNA-183 reduces FOXO1 expression in gastric cancer patients with Helicobacter pylori infection

The aim of the study is to detect the expression of FOXO1 mRNA and protein in samples from gastric cancer patients with Helicobacter pylori (H. pylori) infection, and to investigate the relationship between FOXO1 expression and miR-183 expression. Twenty-six gastric cancer patients with H. pylori infection and 26 gastric cancer patients without H. pylori infection were included into experimental group and control group, respectively. Tumor tissues and peripheral blood were collected from all subjects. QRT-PCR was used to determine the expression of miRNA and mRNA. Western blotting was carried out to measure protein expression. Dual luciferase reporter assay was used to identify direct interaction between miRNA and 3’-UTR of mRNA. Cell proliferation was examined by CCK-8 assay. FOXO1 mRNA and protein expression was downregulated in gastric cancer patients, being possibly related to H. pylori infection. The expression of miR-183 in tumor tissues and serum from gastric cancer patients with H. pylori infection was elevated, and probably regulated the expression of FOXO1 by direct targeting. Stimulation by H. pylori up-regulated the expression of miR-183 in gastric cancer AGS cells, and reduced the levels of FOXO1 mRNA and protein. Inhibition of miR183 elevated the expression of FOXO1 and suppressed the proliferation of AGS cells. The present study demonstrates that the expression of FOXO1 in tumor tissues and blood from gastric cancer patients with H. pylori infection is significantly down-regulated, and may be related to the up-regulation of miR-183. H. pylori may regulate FOXO1 expression through miR-183 to affect the pathological process of gastric cancer

Gas kinematics and star formation in the filamentary molecular cloud G47.06+0.26

We performed a multi-wavelength study toward the filamentary cloud G47.06+0.26 to investigate the gas kinematics and star formation. We present the 12CO (J=1-0), 13CO (J=1-0) and C18O (J=1-0) observations of G47.06+0.26 obtained with the Purple Mountain Observation (PMO) 13.7 m radio telescope to investigate the detailed kinematics of the filament. The 12CO (J=1-0) and 13CO (J=1-0) emission of G47.06+0.26 appear to show a filamentary structure. The filament extends about 45 arcmin (58.1 pc) along the east-west direction. The mean width is about 6.8 pc, as traced by the 13CO (J=1-0) emission. G47.06+0.26 has a linear mass density of about 361.5 Msun/pc. The external pressure (due to neighboring bubbles and H II regions) may help preventing the filament from dispersing under the effects of turbulence. From the velocity-field map, we discern a velocity gradient perpendicular to G47.06+0.26. From the Bolocam Galactic Plane Survey (BGPS) catalog, we found nine BGPS sources in G47.06+0.26, that appear to these sources have sufficient mass to form massive stars. We obtained that the clump formation efficiency (CFE) is about 18% in the filament. Four infrared bubbles were found to be located in, and adjacent to, G47.06+0.26. Particularly, infrared bubble N98 shows a cometary structure. CO molecular gas adjacent to N98 also shows a very intense emission. H II regions associated with infrared bubbles can inject the energy to surrounding gas. We calculated the kinetic energy, ionization energy, and thermal energy of two H II regions in G47.06+0.26. From the GLIMPSE I catalog, we selected some Class I sources with an age of about 100000 yr, which are clustered along the filament. The feedback from the H II regions may cause the formation of a new generation of stars in filament G47.06+0.26.Comment: 10 pages, 11 figures, accepted for publication in A&