Raznolikost antigena nigerijskih sojeva virusa influence konja H3.

Antigenic variation among three recent isolations of equine-2 H3N8 influenza viruses from Ibadan, Nigeria is reported. Antigenic analysis with panels of monoclonal antibodies (mAbs) and polyclonal antisera indicated that the three viruses were antigenically divergent, although they were all H3N8 subtype related to other equine 2 -viruses isolated between 1963 and 1987. Results of virus protein synthesis investigated by pulse-chase experiments showed heterogeneity among the proteins of the ribonucleoprotein complex and the haemagglutinin glycoproteins, which were not cleaved in tissue culture. The results of this study indicate that equine H3 HAs have evolved by a process of evolutionary divergence and mutational changes, as confirmed by genetic analysis in another study. The results also showed that antigenic variation occurs among equine H3 influenza viruses and that H3 viruses with antigenically different HA molecules could co-circulate in equine populations.Obra|ena je raznolikost antigena tri nova izolata nigerijskih sojeva virusa influence konja H3N8 iz Ibadana u Nigeriji. Analiza antigena s pločama monoklonskih protutijela (mAbs) i poliklonskim antiserumima pokazala je da su tri virusa antigenski različiti, iako su svi bili H3N8 podtipa i srodni s drugim virusima influence konja serotipa A2 izoliranim od 1963. do 1987. Rezultati sinteze virusnog proteina istraženi sa "pulse-chase" pokusima pokazali su heterogenost proteina ribonukleinskog kompleksa i hemaglutininskih glikoproteina, a koji nisu rasli u kulturi tkiva. Rezultati ovog istraživanja naznačuju da su konjski H3 HAs nastali procesom evolucijske divergencije i mutacijama, a to je potvr|eno i genetskim analizama u drugoj studiji. Rezultati tako|er pokazuju da se pojavljuju antigene varijacije među H3 virusima konjske influence i da H3 virusi s antigeno različitim HA molekulama mogu zajedno cirkulirati u populacijama konja

Raznolikost antigena nigerijskih sojeva virusa influence konja H3.

Antigenic variation among three recent isolations of equine-2 H3N8 influenza viruses from Ibadan, Nigeria is reported. Antigenic analysis with panels of monoclonal antibodies (mAbs) and polyclonal antisera indicated that the three viruses were antigenically divergent, although they were all H3N8 subtype related to other equine 2 -viruses isolated between 1963 and 1987. Results of virus protein synthesis investigated by pulse-chase experiments showed heterogeneity among the proteins of the ribonucleoprotein complex and the haemagglutinin glycoproteins, which were not cleaved in tissue culture. The results of this study indicate that equine H3 HAs have evolved by a process of evolutionary divergence and mutational changes, as confirmed by genetic analysis in another study. The results also showed that antigenic variation occurs among equine H3 influenza viruses and that H3 viruses with antigenically different HA molecules could co-circulate in equine populations.Obra|ena je raznolikost antigena tri nova izolata nigerijskih sojeva virusa influence konja H3N8 iz Ibadana u Nigeriji. Analiza antigena s pločama monoklonskih protutijela (mAbs) i poliklonskim antiserumima pokazala je da su tri virusa antigenski različiti, iako su svi bili H3N8 podtipa i srodni s drugim virusima influence konja serotipa A2 izoliranim od 1963. do 1987. Rezultati sinteze virusnog proteina istraženi sa "pulse-chase" pokusima pokazali su heterogenost proteina ribonukleinskog kompleksa i hemaglutininskih glikoproteina, a koji nisu rasli u kulturi tkiva. Rezultati ovog istraživanja naznačuju da su konjski H3 HAs nastali procesom evolucijske divergencije i mutacijama, a to je potvr|eno i genetskim analizama u drugoj studiji. Rezultati tako|er pokazuju da se pojavljuju antigene varijacije među H3 virusima konjske influence i da H3 virusi s antigeno različitim HA molekulama mogu zajedno cirkulirati u populacijama konja