A Decomposition of North American Trade Growth since NAFTA

Total U.S. trade with NAFTA partners has increased 78 percent in real terms since 1993-U.S. Mexico trade alone is up 141 percent-compared to a 43 percent increase in U.S. trade with the rest of the world. In this article we compare the nature of U.S. trade growth with Canada and Mexico to growth in U.S. trade with non NAFTA partners. We apply a simple decomposition of trade growth offered by Hummels and Klenow (2002) that provides insights into whether the United States is trading more of the same goods with NAFTA partners since 1993, or trading new products. The results provide evidence of both. A sizeable component of U.S. trade growth since 1993 can be explained by increases in the variety of products the U.S. imports from Mexico.NAFTA, International Trade

The N-Glycosylation of immunoglobulin G as a novel biomarker of Parkinson’s disease

For neurodegenerative diseases, interventions during the early stages of the disease, before significant neurodegeneration has occurred, are associated with an increased probability of slowing or halting the disease process. In order to intervene early, it is essential that an accurate diagnosis is obtained and that disease progression can be monitored. This is particularly relevant for Parkinson’s disease (PD; International Classification of Diseases version 10) because significant neurodegeneration has already occurred by the time the clinical motor symptoms are present. Therefore, the development of translatable, high-throughput biomarkers for large scale population screening is a crucial area of research. Of promise are the emerging “omics” technologies, which enable the detection of preclinical biomolecule fluctuations associated with the development of different diseases. One such field is glycomics which is the study of the set of sugar structures, hereon in known as glycans, in a given protein, cell or tissue. Notably, the functional diversity of proteins is increased by several magnitudes with the addition of glycans, a process known as glycosylation. The glycosylation of certain proteins, including immunoglobulin G (IgG), has been found to remain fairly stable over short periods of time, with modifications thought to result from changes in the cellular environment or disease presence. Indeed, IgG has the ability to exert both anti-inflammatory and pro-inflammatory effects throughout the body and these properties are controlled by the N-glycosylation of the fragment crystallisable (Fc) portion. To our knowledge, this was the first time that the potential of using IgG glycomic biomarkers to identify people with PD, as well as identify people with PD who are at risk of cognitive decline, was investigated. It was demonstrated that the peripheral IgG glycome in the PD cases was indicative of an increased capacity to biologically age. While advancing age has previously been associated with modifications to the glycosylation of IgG, making them more pro-inflammatory, advancing age was only associated with significant increases in modifications to the peripheral IgG glycome that infer more pro-inflammatory IgG in the PD cases but not the controls. In PD, the severity of the underlying pathology increases as the individual ages and, therefore, is a confounder of the effect of advancing age on pro-inflammation. Consequently, the peripheral IgG in people with PD have a propensity to become more pro-inflammatory at a faster rate as they age, and this may be linked to the severity of pathology during the course of the disease. PD has a heterogeneous presentation of clinical symptoms, and many factors contribute to the development of the disease. While this is true, it was demonstrated that the peripheral IgG glycome does not have utility in identifying risk of cognitive decline, which would result from progression of PD pathology in the central nervous system (CNS). These results are indicative of the peripheral IgG interacting with PD pathology in the enteric nervous system (ENS) as well as when it propagates from the ENS to the CNS along the vagal nerve. Inflammation may facilitate the neuron-to-neuron propagation of PD inclusions along this pathway and thus be a contributor to PD development during the prodromal phase. Hence, the peripheral IgG glycome may be useful as a novel biomarker of PD presence in the prodromal phase of the disease

Effects of Pitch and Rhythm Priming Tasks on Accuracy and Fluency during Sight-reading

Given the prevalent use of sight-reading in the classroom, at music festivals, and in audition procedures, it is important to know the most effective practices in preparing students to sight-read musical excerpts. Previous studies suggest that rhythm accuracy is a significant indicator of sight-reading ability. However, others have observed a possible influence of pitch on the performance of rhythm. In an effort to better understand that relationship, the purpose of this study was to investigate the effects of pitch and rhythm priming tasks on sight-reading accuracy and fluency. High school wind instrumentalists (N=182) sight-read selected stimulus exercises from the Watkins-Farnum Performance Scale under one of four conditions: pre/post rhythm, pre/post pitch, post only rhythm, or post only pitch. As part of a repeated measures design, two priming treatments and a control condition were administered. Participants played through either the rhythms on one pitch or through the sequence of pitches on quarter notes during perceptual priming tasks and through either a general rhythm exercise or scale exercise during conceptual priming tasks. Those in pre-test/post-test groups first sight-read the exercise as written while those in the post-test only groups began with treatments. Using a three-way repeated measures MANOVA, no significant differences were found in rhythm, pitch or fluency accuracy based on treatment condition (pitch or rhythm) or exposure condition (pre/post or post only). Significant differences were found based on priming condition (p \u3c .02). Rhythms scores were significantly lower after both perceptual and conceptual priming than after control conditions. No significant differences in pitch accuracy or fluency were detected based on priming condition but each significantly improved over time. These results suggest that rhythm processing was influenced in different ways than pitch. The independent consideration of fluency revealed important relationships between pitch and sight-reading accuracy. In addition, significant differences in pitch were found between brass and woodwind players suggesting the importance of aural representation skills in accurate sight-reading. Based on these outcomes, future research should continue to investigate the complex roles of rhythm and pitch processing during music reading performance tasks

Tropical Tree Species Effects on Soil pH and Biotic Factors and the Consequences for Macroaggregate Dynamics

Physicochemical and biotic factors influence the binding and dispersivity of soil particles, and thus control soil macroaggregate formation and stability. Although soil pH influences dispersivity, it is usually relatively constant within a site, and thus not considered a driver of aggregation dynamics. However, land-use change that results in shifts in tree-species composition can result in alteration of soil pH, owing to species-specific traits, e.g., support of nitrogen fixation and Al accumulation. In a long-term, randomized complete block experiment in which climate, soil type, and previous land-use history were similar, we evaluated effects of individual native tropical tree species on water-stable macroaggregate size distributions in an Oxisol. We conducted this study at La Selva Biological Station in Costa Rica, in six vegetation types: 25-year-old plantations of four tree species grown in monodominant stands; an unplanted Control; and an adjacent mature forest. Tree species significantly influenced aggregate proportions in smaller size classes (0.25–1.0 mm), which were correlated with fine-root growth and litterfall. Tree species altered soil pH differentially. Across all vegetation types, the proportion of smaller macroaggregates declined significantly as soil pH increased (p ≤ 0.0184). This suggests that alteration of pH influences dispersivity, and thus macroaggregate dynamics, thereby playing a role in soil C, N, and P cycling

\u3cem\u3eRhizobium etli\u3c/em\u3e CE3 Bacteroid Lipopolysaccharides Are Structurally Similar but Not Identical to Those Produced by Cultured CE3 Bacteria

Rhizobium etli CE3 bacteroids were isolated from Phaseolus vulgaris root nodules. The lipopolysaccharide (LPS) from the bacteroids was purified and compared with the LPS from laboratory-cultured R. etli CE3and from cultures grown in the presence of anthocyanin. Comparisons were made of the O-chain polysaccharide, the core oligosaccharide, and the lipid A. Although LPS from CE3 bacteria and bacteroids are structurally similar, it was found that bacteroid LPS had specific modifications to both the O-chain polysaccharide and lipid A portions of their LPS. Cultures grown with anthocyanin contained modifications only to the O-chain polysaccharide. The changes to the O-chain polysaccharide consisted of the addition of a single methyl group to the 2-position of a fucosyl residue in one of the five O-chain trisaccharide repeat units.This same change occurred for bacteria grown in the presence of anthocyanin. This methylation change correlated with the inability of bacteroid LPS and LPS from anthocyanin-containing cultures to bind the monoclonal antibody JIM28. The coreoligosaccharide region of bacteroid LPS and from anthocyanin grown cultures was identical to that of LPS from normal laboratory-cultured CE3. The lipid A from bacteroids consisted exclusively of a tetraacylated species compared with the presence of both tetra-and pentaacylated lipid A from laboratory cultures. Growth in the presence of anthocyanin did not affect the lipid A structure. Purified bacteroids that could resume growth were also found to be more sensitive to the cationic peptides, poly-L-lysine, polymyxin-B, and melittin

Christine Hartman Sullivan and Russell Neil Sullivan in a Joint Senior Recital

This is the program for the joint senior recital of clarinetist Christine Hartman Sullivan and baritone Russell Neil Sullivan. Pianist Jamie Coffelt assisted Sullivan; pianist Faron Wilson assisted Sullivan. The recital took place on March 1, 1988, in the Mabee Fine Arts Recital Hall

Customer requirements process

Customer requirements are presented through three viewgraphs. One graph presents the range of services, which include requirements management, network engineering, operations, and applications support. Another viewgraph presents the project planning process. The third viewgraph presents the programs and/or projects actively supported including life sciences, earth science and applications, solar system exploration, shuttle flight engineering, microgravity science, space physics, and astrophysics

CATHEDRAL: A Fast and Effective Algorithm to Predict Folds and Domain Boundaries from Multidomain Protein Structures

We present CATHEDRAL, an iterative protocol for determining the location of previously observed protein folds in novel multidomain protein structures. CATHEDRAL builds on the features of a fast secondary-structure–based method (using graph theory) to locate known folds within a multidomain context and a residue-based, double-dynamic programming algorithm, which is used to align members of the target fold groups against the query protein structure to identify the closest relative and assign domain boundaries. To increase the fidelity of the assignments, a support vector machine is used to provide an optimal scoring scheme. Once a domain is verified, it is excised, and the search protocol is repeated in an iterative fashion until all recognisable domains have been identified. We have performed an initial benchmark of CATHEDRAL against other publicly available structure comparison methods using a consensus dataset of domains derived from the CATH and SCOP domain classifications. CATHEDRAL shows superior performance in fold recognition and alignment accuracy when compared with many equivalent methods. If a novel multidomain structure contains a known fold, CATHEDRAL will locate it in 90% of cases, with <1% false positives. For nearly 80% of assigned domains in a manually validated test set, the boundaries were correctly delineated within a tolerance of ten residues. For the remaining cases, previously classified domains were very remotely related to the query chain so that embellishments to the core of the fold caused significant differences in domain sizes and manual refinement of the boundaries was necessary. To put this performance in context, a well-established sequence method based on hidden Markov models was only able to detect 65% of domains, with 33% of the subsequent boundaries assigned within ten residues. Since, on average, 50% of newly determined protein structures contain more than one domain unit, and typically 90% or more of these domains are already classified in CATH, CATHEDRAL will considerably facilitate the automation of protein structure classification

Deployment and Impact of Support Staff in Schools : The Impact of Support Staff in Schools (Results from Strand 2, Wave 2)

This study was designed to obtain up to date and reliable data on the deployment and characteristics of support staff and the impact of support staff on pupil outcomes and teacher workloads. The study covered schools in England and Wales. It involved large scale surveys (Strand 1), followed by a multi-method and multi informant approach (Strand 2). It provided detailed baseline data by which to assess change and progress over time. It sought to understand the processes in schools which lead to the effective use of support staff. The DISS project was funded by the Department for Children, Schools and Families (DCSF) and Welsh Assembly Government (WAG)