The flagellin of candidate live biotherapeutic Enterococcus gallinarum MRx0518 is a potent immunostimulant

Abstract Many links between gut microbiota and disease development have been established in recent years, with particular bacterial strains emerging as potential therapeutics rather than causative agents. In this study we describe the immunostimulatory properties of Enterococcus gallinarum MRx0518, a candidate live biotherapeutic with proven anti-tumorigenic efficacy. Here we demonstrate that strain MRx0518 elicits a strong pro-inflammatory response in key components of the innate immune system but also in intestinal epithelial cells. Using a flagellin knock-out derivative and purified recombinant protein, MRx0518 flagellin was shown to be a TLR5 and NF-κB activator in reporter cells and an inducer of IL-8 production by HT29-MTX cells. E. gallinarum flagellin proteins display a high level of sequence diversity and the flagellin produced by MRx0518 was shown to be more potent than flagellin from E. gallinarum DSM100110. Collectively, these data infer that flagellin may play a role in the therapeutic properties of E. gallinarum MRx0518

Data Management in Practice Results and Evaluation

 This report presents the results of the Data Management i Praksis (DMiP) project (in English: Data Management in Practice). The project was funded by Denmark’s Electronic Research Library (DEFF), the National Danish Archives and the participating main Danish libraries. The following partners participated in the project: Aalborg University Library, Danish e-Infrastructure Cooperation (DeIC), DTU Library, Roskilde University Library, The Danish National Archives, Royal Danish Library and The University Library of Southern Denmark. The general objective of the project was to establish a Danish setup covering all aspects of the lifecycle of research data: from application, through the research phase, and finally to the dissemination of results and sharing of research data. The setup was to be based on researchers’ demands, and the suggestions and results of the project were to be at an international level. The project should also demonstrate that research libraries have a role to play regarding research data. Furthermore, the project should ensure development of competences at the libraries, which can then be used in the future process of managing research data.

Data Management in Practice Supplementary Files

This collection of supplementary files relate to the report: “Data Management in Practice Results and Evaluation”. The report presents the results of the Data Management i Praksis (DMiP) project (in English: Data Management in Practice). The project was funded by Denmark’s Electronic Research Library (DEFF), the National Danish Archives and the participating main Danish libraries. The following partners participated in the project: Aalborg University Library, Danish e-Infrastructure Cooperation (DeIC), DTU Library, Roskilde University Library, The Danish National Archives, Royal Danish Library and The University Library of Southern Denmark.The general objective of the project was to establish a Danish setup covering all aspects of the lifecycle of research data: from application, through the research phase, and finally to the dissemination of results and sharing of research data. The setup was to be based on researchers’ demands, and the suggestions and results of the project were to be at an international level. The project should also demonstrate that research libraries have a role to play regarding research data. Furthermore, the project should ensure development of competences at the libraries, which can then be used in the future process of managing research data