Разработка алгоритмического и программного обеспечения модуля автоматизированного извлечения контента веб-ресурсов

Проектирование и разработка программного модуля для извлечения данных с веб-ресурсов для исследования общественного мнения. Разработанный модуль осуществляет сбор данных с веб-ресурсов, по методам RSS-запросов и фреймворка Twitter4J, а также предварительную обработку данных по средствам синтаксического анализа. Общение с другими модулями происходит через базу данных в которую происходит выгрузка данных после предварительной обработки.Designing and developing a software module for retrieving data from web resources for research of public opinion. The module developed collects data from web resources, RSS-queries methods and Twitter4J framework, as well as pre-processing data on parsing tools. Communication with other modules occurs through the database in which the data is downloaded after pre-processing

Design of virtual polygon in Unity3D

The paper is devoted to research and designing of autonomous car - a self-governing car in which artificial intelligence will take the role of driver. In today's world, the present invention is necessary because today humanity cannot imagine being without any types of transport

Identification of global inhibitors of cellular glycosylation

Small molecule inhibitors of glycosylation enzymes are valuable tools for dissecting glycan functions and potential drug candidates. Screening for inhibitors of glycosyltransferases are mainly performed by in vitro enzyme assays with difficulties moving candidates to cells and animals. Here, we circumvent this by employing a cell-based screening assay using glycoengineered cells expressing tailored reporter glycoproteins. We focused on GalNAc-type O-glycosylation and selected the GalNAc-T11 isoenzyme that selectively glycosylates endocytic low-density lipoprotein receptor (LDLR)-related proteins as targets. Our screen of a limited small molecule compound library did not identify selective inhibitors of GalNAc-T11, however, we identify two compounds that broadly inhibited Golgi-localized glycosylation processes. These compounds mediate the reversible fragmentation of the Golgi system without affecting secretion. We demonstrate how these inhibitors can be used to manipulate glycosylation in cells to induce expression of truncated O-glycans and augment binding of cancer-specific Tn-glycoprotein antibodies and to inhibit expression of heparan sulfate and binding and infection of SARS-CoV-2

Автоматизированная система управления бизнес-процессами на базе малого предприятия

Проектирование системы автоматизированного управления бизнес-процессами на базе малого предприятия по производству пластика для 3D-печати. Объектом исследования является бизнес-процессы, протекающие на предприятии по производству пластика для 3D-печати Целью работы является разработка системы управления бизнес-процессами.Designing an automated business process management system based on a small plastic manufacturing enterprise for 3D printin

Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences

Enterovirus D68 (EV-D68) is an emerging pathogen associated with mild to severe respiratory disease. Since 2014, EV-D68 is also linked to acute flaccid myelitis (AFM), causing paralysis and muscle weakness in children. However, it remains unclear whether this is due to an increased pathogenicity of contemporary EV-D68 clades or increased awareness and detection of this virus. Here, we describe an infection model of primary rat cortical neurons to study the entry, replication, and functional consequences of different EV-D68 strains, including historical and contemporary strains. We demonstrate that sialic acids are important (co)receptors for infection of both neurons and respiratory epithelial cells. Using a collection of glycoengineered isogenic HEK293 cell lines, we show that sialic acids on either N-glycans or glycosphingolipids can be used for infection. Additionally, we show that both excitatory glutamatergic and inhibitory GABA-ergic neurons are susceptible and permissive to historical and contemporary EV-D68 strains. EV-D68 infection of neurons leads to the reorganization of the Golgi-endomembranes forming replication organelles, first in the soma and later in the processes. Finally, we demonstrate that the spontaneous neuronal activity of EV-D68-infected neuronal network cultured on microelectrode arrays (MEA) is decreased, independent of the virus strain. Collectively, our findings provide novel insights into neurotropism and -pathology of different EV-D68 strains, and argue that it is unlikely that increased neurotropism is a recently acquired phenotype of a specific genetic lineage. IMPORTANCE Acute flaccid myelitis (AFM) is a serious neurological illness characterized by muscle weakness and paralysis in children. Since 2014, outbreaks of AFM have emerged worldwide, and they appear to be caused by nonpolio enteroviruses, particularly enterovirus-D68 (EV-D68), an unusual enterovirus that is known to mainly cause respiratory disease. It is unknown whether these outbreaks reflect a change of EV-D68 pathogenicity or are due to increased detection and awareness of this virus in recent years. To gain more insight herein, it is crucial to define how historical and circulating EV-D68 strains infect and replicate in neurons and how they affect their physiology. This study compares the entry and replication in neurons and the functional consequences on the neural network upon infection with an old "historical" strain and contemporary "circulating" strains of EV-D68

Synchronisationsmechanismen und Kommunikationsstrukturen zur Steuerung Flexibler Fertigungssysteme

Wien, Technische Universität, Dissertatio

Bildungsmarketing für E-Learning-Kurse von Hochschulen und Firmen

Was ist der Unterschied zwischen E-Learning-Kursen an Hochschulen und in Firmen sowie dem damit verbundenen Bildungsmarketing? An der FH Burgenland, Department Informationstechnologie und -management führen wir E-Learning-Projekte mit Firmen durch, verfügen aber auch über eine breite Erfahrung mit E-Learning im Hochschulkontext, insbesondere im Masterstudiengang Angewandtes Wissensmanagement gesammelt. Basierend auf diesen Erfahrungen stellen wir in diesem Beitrag die Unterschiede zwischen E-Learning-Kursen von Hochschulen und Firmen dar. Dafür gehen wir von der Annahme aus, dass wir einen E-Learning-Kurs mit denselben Inhalten einmal für eine Hochschule und einmal für eine Firma konzipieren.Informationstechnologie und Informationsmanagemen