Effect of Vanadyl Sulfate in Beta Pancreas Celis of Mice With Diabetes Mellitus

The aim of this study is to examine biochemical and histological perspective, whether vanadyl sulfate has an effect on the beta pancreas cells of streptozotocininduced diabetic mice.The diabetic model was elicited by twice intraperitoneal injection of streptozotocin 100 mg/kg followed with 50mg/kg on day 14. Diabetes occurred on day 21 after streptozotocin injection and glucose level raised from 162,2 ± 21,3 mg/dL to 184,3 ± 27,1. After the treatment of vanadyl sulfate once a day for 7 days, blood glucose level were reduce significantly (5 mg/kg, p = 0,013; 30 mg/kg, p <0,001). On day 28 pancreas tissue harvest from normal mice, streptozotocininduced diabetic mice and diabetic mice given vanadyl sulfate. Tissue sections were stained using hematoxylin-eosin and aldehid fuschin. In pancreatic islet of diabetic group, a damage islet, decline islet size, degenerative and apoptosis beta pancreas cell was observed. While pancreatic of diabetic group given vanadyl sulfate showed amelioration in islet cell. Apoptosis beta cells were reduce, andproliferative cells were appear. Islet become intact and the size was increase closed on normal mice

Pengukuran Tingkah Laku pada Model Nyeri Neuropatik Perifer: Tikus dengan CCI (Chronic Constriction Injury)

Neuropathic pain occurs in 20% of patients with chronic pain. This mainly due to the lack of effective treatment and the presence of drug’s side effects. Animal models have been broadly utilized in validating the therapeutic target and the development of analgesic drugs. Chronic constriction injury (CCI) is a model of peripheral neuropathic pain characterized by allodynia and hyperalgesia. Chronic constriction injury model is produced by loose ligation of the sciatic nerve resulting in nerve damage, thus sensitizes and lowers pain threshold. The pain threshold test is performed by providing a pressure and thermal sensory stimulus that results in observable withdrawal behavior. This article discusses several methods in assessing pain-related behavior on the CCI neuropathic pain model

Selective Serotonin Reuptake Inhibitor Fluvoxamine Ameliorates Stress-and NSAID-Induced Peptic Ucler Possibly by involving Hsp70

Background: Selective serotonin reuptake inhibitors (SSRIs) have recently become potential candidates for a new therapeutic approach to ulcer and gastric bleeding. Heat shock protein 70 (Hsp70) plays an important role in cellular resistance to nonsteroidal anti-inflammatory drugs (NSAIDs). However, there is lack of evidence that fluvoxamine recruits Hsp70 to affect stress-induced gastric ulcer. Therefore, we investigated the effect of fluvoxamine on NSAID- and stress-induced gastric ulcer and the possible involvement of Hsp70. Methods: ICR mice were used in the study. Stress induction was made by the water-immersion-plus-restraint method. NSAID-induced gastric ulcer was produced by oral administration of indomethacin. Fluvoxamine was given orally 30 min before stress induction and indomethacin treatment. Results: Stress and indomethacin treatment significantly increased the ulcer index and intraluminal bleeding score. Stress and indomethacin treatment also significantly increased the expression of Hsp70. Fluvoxamine significantly decreased the ulcer index and intraluminal bleeding in both ulcer models. Moreover, fluvoxamine further increased the expression of Hsp70 in the gastric tissue of stress- and indomethacin-treated mice. Conclusions: Our results indicate that fluvoxamine may have a protective effect against stress- as well as NSAID induced gastric ulcer. In addition, the present stud