447 research outputs found
Exact Master Equation and Non-Markovian Decoherence for Quantum Dot Quantum Computing
In this article, we report the recent progress on decoherence dynamics of
electrons in quantum dot quantum computing systems using the exact master
equation we derived recently based on the Feynman-Vernon influence functional
approach. The exact master equation is valid for general nanostructure systems
coupled to multi-reservoirs with arbitrary spectral densities, temperatures and
biases. We take the double quantum dot charge qubit system as a specific
example, and discuss in details the decoherence dynamics of the charge qubit
under coherence controls. The decoherence dynamics risen from the entanglement
between the system and the environment is mainly non-Markovian. We further
discuss the decoherence of the double-dot charge qubit induced by quantum point
contact (QPC) measurement where the master equation is re-derived using the
Keldysh non-equilibrium Green function technique due to the non-linear coupling
between the charge qubit and the QPC. The non-Markovian decoherence dynamics in
the measurement processes is extensively discussed as well.Comment: 15 pages, Invited article for the special issue "Quantum Decoherence
and Entanglement" in Quantum Inf. Proces
Tenacibaculum aiptasiae sp nov., isolated from a sea anemone Aiptasia pulchella
A novel bacterial strain, designated a4(T), isolated from a sea anemone (Aiptasia pulchella) in Taiwan, was characterized using a polyphasic taxonomic approach. Strain a4(T) was aerobic, Gram-negative, pale-yellow-pigmented and rod-shaped. It grew optimally at 30-35 degrees C, in the presence of 3-4 % (w/v) NaCl and at pH 8.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Tenacibaculum (family Flavobacteriaceae, phylum Bacteroidetes). The closest neighbours were Tenacibaculum lutimaris TF-26(T) (97.6 % similarity) and Tenacibaculum aestuarii SMK-4(T) (97.7 % similarity). The novel isolate could be distinguished from all Tenacibaculum species by several phenotypic characteristics. The major fatty acids were summed feature 3 (comprising C-16:1 omega 7c and/or iso-C-15:0 2-OH, 19.6%), iso-C-15:0 (12.9%), iso-C-16:0 3-OH (10.2 %), iso-C-17:0 3-OH (9.9%) and iSO-C-15:1 (9.5 %). The DNA G + C content was 35.0 mol%. Hence, genotypic and phenotypic data demonstrate that strain a4(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum aiptasiae sp. nov. is proposed. The type strain is a4(T) (=BCRC 17655(T) =LMG 24004(T))
Azonexus hydrophilus sp nov., a nifH gene-harbouring bacterium isolated from freshwater
Three Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1(T), d8-2 and IMCC1716, were isolated from a freshwater spring sample and a eutrophic freshwater pond. Based on characterization using a polyphasic approach, the three strains showed highly similar phenotypic, physiological and genetic characteristics. All of the strains harboured the nitrogenase gene nifH, but nitrogen-fixing activities could not be detected in nitrogen-free culture media. The three strains shared 99.6-99.7 % 16S rRNA gene sequence similarity and showed 89-100 % DNA-DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains d8-1(T), d8-2 and IMCC1716 formed a monophyletic branch in the periphery of the evolutionary radiation occupied by the genus Azonexus. Their closest neighbours; were Azonexus caeni Slu-05(T) (96.7-96.8% similarity) and Azonexus fungiphilus BS5-8(T) (96.3-96.6 %). The DNA-DNA relatedness of the novel strains to these two species of the genus Azonexus was less than 70%. The isolates could also be differentiated from recognized members of the genus Azonexus on the basis of phenotypic and biochemical characteristics. It is evident, therefore, that the three strains represent a novel species of the genus Azonexus, for which the name Azonexus hydrophilus sp. nov. is proposed. The type strain is d8-1(T) (=LMG 24005(T)=BCRC 17657 (T))
Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine
Bacillus kaustophilus leucine aminopeptidase (bkLAP) was sensitive to oxidative damage by hydrogen peroxide. To improve its oxidative stability, the oxidation-sensitive methionine residues in the enzyme were replaced with leucine by site-directed mutagenesis. The variants, each with an apparent molecular mass of approximately 54 kDa, were overexpressed in recombinant Escherichia coli M15 cells and purified to homogeneity by nickel-chelate chromatography. The specific activity for M282L, M285L, M289L and M321L decreased by more than 43%, while M400L, M426L, M445L, and M485L showed 191, 79, 313, and 103%, respectively, higher activity than the wild-type enzyme. Although the mutations did not cause significant changes in the K-m value, more than 67.8% increase in the value of k(cat)/K-m was observed in the M400L, M426L, M445L and M485L. In the presence of 50 mM H2O2 most variants were more stable with respect to the wild-type enzyme, indicating that the oxidative stability of the enzyme can be improved by engineering the methionine residues
A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223
Two degenerate primers established from the consensus sequences of bacterial leucine aminopeptidases (LAP) were used to amplify a 360-bp gene fragment from the chromosomal DNA of thermophilic Bacillus kaustophilus CCRC 11223 and the amplified fragment was successfully used as a probe to clone a leucine aminopeptidase (lap) gene from a genomic library of the strain. The gene consists of an open reading frame (ORF) of 1,494 bp and encodes a protein of 497 amino acid residues with a calculated molecular mass of 53.7 kDa. The complete amino acid sequence of the cloned enzyme showed greater than 30% identity with prokaryotic and eukaryotic LAPs. Phylogenetic analysis showed that B. kaustophilus LAP is closely related to the enzyme from Bacillus subtilis and is grouped with the M17 family. His(6)-tagged LAP was generated in Escherichia coli by cloning the coding region into pQE-30 and the recombinant enzyme was purified by nickel-chelate chromatography. The pH and temperature optima for the purified enzyme were 8 and 65degreesC, respectively, and 50% of its activity remained after incubation at 60degreesC for 32 min. The enzyme preferentially hydrolyzed L-leucine-p-nitroanilide (L-Leu-p-NA) followed by Cys derivative
Water wave propagation and scattering over topographical bottoms
Here I present a general formulation of water wave propagation and scattering
over topographical bottoms. A simple equation is found and is compared with
existing theories. As an application, the theory is extended to the case of
water waves in a column with many cylindrical steps
Effects of built environment on walking among Hong Kong older adults
Key Messages1. Reliable and valid intervieweradministered questionnaires were developed to investigate associations of perceived neighbourhood attributes of Hong Kong older adults with their walking for transportation and recreation.2. Access to and availability of different types of services and destinations, provision offacilities for resting/sitting in the neighbourhood, and easy access to/from residentialbuildings may help maintain an active lifestyle by facilitating walking for transport in the neighbourhood.3. Access to services, indoor places for walking, environmental aesthetics, low traffic, and absence of physical barriers may promote recreational walking
A Single Laser System for Ground-State Cooling of 25-Mg+
We present a single solid-state laser system to cool, coherently manipulate
and detect Mg ions. Coherent manipulation is accomplished by
coupling two hyperfine ground state levels using a pair of far-detuned Raman
laser beams. Resonant light for Doppler cooling and detection is derived from
the same laser source by means of an electro-optic modulator, generating a
sideband which is resonant with the atomic transition. We demonstrate
ground-state cooling of one of the vibrational modes of the ion in the trap
using resolved-sideband cooling. The cooling performance is studied and
discussed by observing the temporal evolution of Raman-stimulated sideband
transitions. The setup is a major simplification over existing state-of-the-art
systems, typically involving up to three separate laser sources
Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia
Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T))
Burkholderia nodosa sp nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
Three strains, Br3437(T), Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437(T)) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437(T) (=LMG 23741(T) =BCRC 17575(T)), was isolated from nodules of M. scabrella
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