13 research outputs found
Combined microsatellite and FGFR3 mutation analysis enables a highly sensitive detection of urothelial cell carcinoma in voided urine
PURPOSE: Fibroblast growth factor receptor 3 (FGFR3) mutations were
reported recently at a high frequency in low-grade urothelial cell
carcinoma (UCC). We investigated the feasibility of combining
microsatellite analysis (MA) and the FGFR3 status for the detection of UCC
in voided urine. EXPERIMENTAL DESIGN: In a prospective setting, 59 UCC
tissues and matched urine samples were obtained, and subjected to MA (23
markers) and FGFR3 mutation analysis (exons 7, 10, and 15). In each case,
a clinical record with tumor and urine features was provided. Fifteen
patients with a negative cystoscopy during follow-up served as controls.
RESULTS: A mutation in the FGFR3 gene was found in 26 (44%) UCCs of which
22 concerned solitary pTaG1/2 lesions. These mutations were absent in the
15 G3 tumors. For the 6 cases with leukocyturia, 46 microsatellite
alterations were found in the tumor. Only 1 of these was also detected in
the urine. This was 125 of 357 for the 53 cases without leukocyte
contamination. The sensitivity of MA on voided urine was lower for
FGFR3-positive UCC (15 of 21; 71%) as compared with FGFR3 wild-type UCC
(29 of 32; 91%). By including the FGFR3 mutation, the sensitivity of
molecular cytology increased to 89% and was superior to the sensitivity of
morphological cytology (25%) for every clinical subdivision. The
specificity was 14 of 15 (93%) for the two (molecular and morphological)
cytological approaches. CONCLUSIONS: Molecular urine cytology by MA and
FGFR3 mutation analysis enables a highly sensitive and specific detection
of UCC. The similarity of molecular profiles in tumor and urine
corroborate their clonal relation
Use of Three Additional Mid Biopsies to Improve Local Assessment of Prostate Cancer in Patients with One Positive Sextant Biopsy
Nondissection of Pelvic Lymph Nodes Does Not Influence the Results of Perineal Radical Prostatectomy in Selected Patients
P-AKT PATHWAY ACTIVATION AND INHIBITION DEPENDS ON N-CADHERIN OR P-EGFR EXPRESSION IN INVASIVE HUMAN BLADDER CANCER CELL LINES
Computer assisted image analysis of bladder tumour nuclei for morphonuclear and ploidy assessment
Morphonuclear analysis using a quantitative image analysis system has been demonstrated to be a potentially useful technique in the prognostic evaluation of bladder carcinoma. The integrated optical density parameter permits DNA content evaluation in addition to 15 other morphonuclear parameters. We assessed the reliability of morphonuclear analysis by image analysis and flow cytometry for 46 bladder carcinomas and 14 normal bladder specimens. Frozen sample material was obtained from endoscopic resection, radical cystectomy and from cadaveric donors. The grade and staging of the tumours according to the World Health Organization was as follows: 8 G1, 18 G2, 20 G3 and 28 T1, 7 T2, 7 T3, 4 T4. Quantitative image analysis was made on imprint smears stained by the Feulgen method. Simultaneously cell suspensions were obtained by mechanical dissociation and stained with propidium iodide for Flow cytometry analysis. There was a good agreement between quantitative image analysis and flow cytometry for DNA content measurement indicating that image analysis is a reliable method for the quantitation of DNA content (P = 0.001). Moreover we found a good correlation between five of the morphonuclear parameters: surface (P < 0.001), chromatin clumps distribution (P < 0.001), frequency of small (P < 0.001) to large chromatin clumps (P < 0.001) and the grade of bladder tumours. These results indicate that morphonuclear analysis may be a valuable method of quantitating DNA and morphonuclear parameters in a single analysis to provide information which may have some prognostic significance for patients with bladder carcinoma. © 1994.SCOPUS: ar.jinfo:eu-repo/semantics/publishe