Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs

10.1093/nar/gkp857Nucleic Acids Research381215-22

Tyrosine phosphorylation profiling in FGF-2 stimulated human embryonic stem cells

10.1371/journal.pone.0017538PLoS ONE63

Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs

10.1186/1479-5876-9-47Journal of Translational Medicine9

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical research.Peer reviewe

Parameters influencing the expression of mature glial-cell-line-derived neurotrophic factor in Escherichia coli

Biotechnology and Applied Biochemistry253223-233BABI

Normalized median fluorescence: An alternative flow cytometry analysis method for tracking human embryonic stem cell states during differentiation

10.1089/ten.tec.2012.0150Tissue Engineering - Part C: Methods192156-16

Defined and serum-free media support undifferentiated human embryonic stem cell growth

10.1089/scd.2009.0210Stem Cells and Development196753-761SCDT

Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism

10.3109/14653249.2011.635853Cytotherapy143274-284CYTR

Temporal application of topography to increase the rate of neural differentiation from human pluripotent stem cells

10.1016/j.biomaterials.2012.09.033Biomaterials342382-392BIMA

Excess of O-GlcNAc modifies human pluripotent stem cells differentiation

International audienc