Biomechanical Study of 3 Osteoconductive Materials Applied in Pedicle Augmentation and Revision for Osteoporotic Vertebrae: Allograft Bone Particles, Calcium Phosphate Cement, Demineralized Bone Matrix

Objective This study assessed biomechanical properties of pedicle screws enhanced or revised with 3 materials. We aimed to compare the efficacy of these materials in pedicle augmentation and revision. Methods One hundred twenty human cadaveric vertebrae were utilized for in vitro testing. Vertebrae bone density was evaluated. Allograft bone particles (ABP), calcium phosphate cement (CPC), and demineralized bone matrix (DBM) were used to augment or revise pedicle screw. Post the implantation of pedicle screws, parameters such as insertional torque, pullout strength, cycles to failure and failure load were measured using specialized instruments. Results ABP, CPC, and DBM significantly enhanced biomechanical properties of the screws. CPC augmentation showed superior properties compared to ABP or DBM. ABP-augmented screws had higher cycles to failure and failure loads than DBM-augmented screws, with no difference in pullout strength. CPC-revised screws exhibited similar strength to the original screws, while ABP-revised screws showed comparable cycles to failure and failure loads but lower pullout strength. DBM-revised screws did not match the original screws’ strength. Conclusion ABP, CPC, and DBM effectively improve pedicle screw stability for pedicle augmentation. CPC demonstrated the highest efficacy, followed by ABP, while DBM was less effective. For pedicle revision, CPC is recommended as the primary choice, with ABP as an alternative. However, using DBM for pedicle revision is not recommended

Numerical Analysis for Wetting Behaviors of an Oil Jet Lubricated Spur Gear

As it is widely employed in the aeronautical transmission system, a better understanding of the oil jet lubrication behavior is vital to determine the total system energy consumption. Firstly, this study presents related theoretical models such as the sum of oil jet resistance torque, impingement depth, and wetted area of the oil film for calibrating the physical characteristics of the impact of the oil jet on the gear flank. Then, in terms of the flow phenomenology of the liquid column for the oil jet impact on an isolated spur gear, a detailed transient and spatial flow field analysis becomes available, benefiting from an overset mesh method integrating with a volume-of-fluid (VOF) method. Furthermore, not only the oil jet resistance torque, but also the impingement depth as well as the spatial and temporal evolution of wetted surface by the oil film on the gear tooth given by numerical investigations were compared well with the theoretical calculations

Numerical Analysis for Wetting Behaviors of an Oil Jet Lubricated Spur Gear

As it is widely employed in the aeronautical transmission system, a better understanding of the oil jet lubrication behavior is vital to determine the total system energy consumption. Firstly, this study presents related theoretical models such as the sum of oil jet resistance torque, impingement depth, and wetted area of the oil film for calibrating the physical characteristics of the impact of the oil jet on the gear flank. Then, in terms of the flow phenomenology of the liquid column for the oil jet impact on an isolated spur gear, a detailed transient and spatial flow field analysis becomes available, benefiting from an overset mesh method integrating with a volume-of-fluid (VOF) method. Furthermore, not only the oil jet resistance torque, but also the impingement depth as well as the spatial and temporal evolution of wetted surface by the oil film on the gear tooth given by numerical investigations were compared well with the theoretical calculations

Numerical Analysis for Wetting Behaviors of an Oil Jet Lubricated Spur Gear

As it is widely employed in the aeronautical transmission system, a better understanding of the oil jet lubrication behavior is vital to determine the total system energy consumption. Firstly, this study presents related theoretical models such as the sum of oil jet resistance torque, impingement depth, and wetted area of the oil film for calibrating the physical characteristics of the impact of the oil jet on the gear flank. Then, in terms of the flow phenomenology of the liquid column for the oil jet impact on an isolated spur gear, a detailed transient and spatial flow field analysis becomes available, benefiting from an overset mesh method integrating with a volume-of-fluid (VOF) method. Furthermore, not only the oil jet resistance torque, but also the impingement depth as well as the spatial and temporal evolution of wetted surface by the oil film on the gear tooth given by numerical investigations were compared well with the theoretical calculations.</jats:p

CFD investigation of air-oil two-phase flow in oil jet nozzle

In aero-engines applications, nozzle geometric parameters have major effects on the lubricating oil to accurately reach target gears or bearings. To investigate the deviation phenomenon of oil jet flow in the lubrication system, the computational fluid dynamics (CFD) technique integrating with the volume of fluid (VOF) method and SST k- ω turbulence model is adopted to deeply understand the flow characteristics of the nozzle. By leveraging this method, the relationship between nozzle geometry, inlet pressure, jet velocity, and jet deviation are firstly analyzed. The results reveal that a higher average velocity and smaller jet deviation can be determined via adjusting the nozzle geometry. Furthermore, a specific test rig, composed of an oil supply system, target system and data collection system, is set up for oil injection test; experimental outlet velocity and mass flow rate of oil passing through the hole on the target plate are monitored and recorded. The experimental findings compare well with numerical results obtained by the CFD method. </jats:p

miRNA143 Downregulated the Expression of Cyclophilin A to Inhibit the Progression of Atherosclerosis

To investigate the influence of microRNA (miR)-143 on atherosclerosis and its working mechanism via cyclophilin A. Human acute monocytic leukemia cells (human acute monocytic leukemia cells, human THP-1 monocytes) were treated with ox-LDL 80 mg/L for 48 h to establish a foam cell model. The modeling was evaluated by oil red O staining. The expressions levels of miRNA-143 and CyPA were detected by q-PCR. RNA extracted was confirmed by SiO2 nano magnetic bead. Dual-luciferase reporter gene assay was used to verify the targeted regulatory relationship between miRNA-143 and CyPA. Next, the cells were transfected with miR-143 mimics and treated with a CyPA inhibitor (cyclophilin A). The expression levels of CyPA and downstream matrix metalloproteinases-9 (MMP-9) in the downstream were detected by q-PCR and western blot, respectively. No or few lipid droplets were observed in the normal THP-1 cells. After treatment with ox-LDL for 48 h, a large amount of red-stained lipid was observed in the cytoplasm, indicating that the THP-1 derived foam cell models were successfully constructed. q-PCR results indicated that miRNA-143 was significantly downregulated in the THP-1-derived foam cell model, whereas CyPA was upregulated (P &lt; 0.05); the dual luciferase reporter gene assay was performed for miRNA-143 and 3'-UTR of CyPA, and the fluorescence intensity of the 3'-UTR vector group was significantly lower than that of the empty vector group (P &lt; 0.05). Fluorescence intensity detection of mutations at the target site showed that it had no effect was produced on the fluorescence intensity. This implied a negative regulatory effect conferred of miRNA-143 on CyPA; As compared to the model group, transfection with miRNA-143 mimics led to a significant downregulation of CyPA mRNA (P &lt; 0.05), which was accompanied by significant downregulation of both MMP-9 mRNA and protein (P &lt; 0.05). MMP-9 was also significantly downregulated upon the addition of the CyPA inhibitor cyclosporine A (P &lt; 0.05).</jats:p