Rural-Urban Transition and Food Security in India (MiFood Paper No. 12)

As a growing proportion of world’s population lives in cities and towns, food security is increasingly acquiring an urban character. The locus of food security research and policy agendas has correspondingly expanded from rural areas to include cities and towns in the past few years. However, the dominant discourse on urbanization-food security relationship appears to be shaped by perspectives from the Global North and large cities, and shows a lack of adequate understanding of the urbanization-food security nexus in the small towns of the Global South. This paper aims to correct this bias. With a focus on India where urban growth is increasingly concentrated in small, former rural regions, this paper reviews the food and nutrition security implications of the country’s rural-urban transition. It identifies three conceptual pathways through which to understand the bearing of rural-urban transition on food and nutrition security that include: livelihood change, land use change, and dietary change. The evidence reviewed suggests the overall worsening of food and nutrition security for people in this rural-urban transition, particularly for the poor populations. The paper also identifies several key research questions and calls for more research on the urbanization-food security nexus in India

Construction of oligonucleotide arrays on a glass surface using a heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA)

A rapid method for construction of oligonucleotide arrays on a glass surface, using a novel heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA), has been described. The heterobifunctional reagent, NTMTA, carries two different thermoreactive groups. The triethoxysilyl group on one end is specific towards silanol functions on the virgin glass surface, while the trifluoroethanesulfonyl (tresyl) group on the other end of the reagent reacts specifically with aminoalkyl- or mercaptoalkyl- functionalized oligonucleotides. Immobilization of oligonucleotides on a glass surface has been realized via two routes. In the first one (A), 5′- aminoalkyl- or mercaptoalkyl-functionalized oligonucleotides were allowed to react with NTMTA to form a oligonucleotide-triethoxysilyl conjugate which, in a subsequent reaction with unmodified (virgin) glass microslide, results in surface-bound oligonucleotides. In the second route (B), the NTMTA reagent reacts first with a glass microslide whereby it generates trifluoroethanesulfonate ester functions on it, which in a subsequent step react with 5′-aminoalkyl or mercaptoalkyl oligonucleotides to generate support-bound oligonucleotides. Subsequently, the oligonucleotide arrays prepared by both routes were analyzed by hybridization experiments with complementary oligonucleotides. The constructed microarrays were successfully used in single and multiple nucleotide mismatch detection by hybridizing these with fluorescein-labeled complementary oligonucleotides. Further more, the proposed method was compared with the existing methods with respect to immobilization efficiency of oligonucleotides