Comparisons of ELISA and Western blot assays for detection of autophagy flux

We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blotting, and compared their working range, accuracy, and reliability. The ELISAs showed a broader working range than that of the LC3 Western blots (Table 1). Table 2 showed that data value distribution was tighter and the average standard error from the ELISA was much smaller than those of the Western blot, directly relating to the accuracy of the assay. Test-retest reliability analysis showed good reliability for three individual ELISAs (interclass correlation, ≥ 0.7), but poor reliability for three individual Western blots (interclass correlation, ≤ 0.4) (Table 3). Keywords: Autophagy, Mitophagy, ELISA, Western blot, Skeletal muscl

Analysis of Laser ARPES from Bi2_2Sr2_2CaCu2_2O8+δ_{8+\delta} in superconductive state: angle resolved self-energy and fluctuation spectrum

We analyze the ultra high resolution laser angle resolved photo-emission spectroscopy (ARPES) intensity from the slightly underdoped Bi$_2$Sr$_2$CaCu$_2$O$_{8+\delta}$ in the superconductive (SC) state. The momentum distribution curves (MDC) were fitted at each energy \w employing the SC Green's function along several cuts perpendicular to the Fermi surface with the tilt angle $\theta$ with respect to the nodal cut. The clear observation of particle-hole mixing was utilized such that the complex self-energy as a function of $\omega$ is directly obtained from the fitting. The obtained angle resolved self-energy is then used to deduce the Eliashberg function \alpha^2 F^{(+)}(\th,\w) in the diagonal channel by inverting the d-wave Eliashberg equation using the maximum entropy method. Besides a broad featureless spectrum up to the cutoff energy $\omega_c$, the deduced $\alpha^2 F$ exhibits two peaks around 0.05 eV and 0.015 eV. The former and the broad feature are already present in the normal state, while the latter emerges only below $T_c$. Both peaks become enhanced as $T$ is lowered or the angle $\th$ moves away from the nodal direction. The implication of these findings are discussed.Comment: 7 pages, 5 figures, summited to PR

Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells

<p>Abstract</p> <p>Background</p> <p>Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation. These microvesicles can mediate communication between cells and affect various tumor-related processes in their target cells.</p> <p>Results</p> <p>We present potential roles of CRC cell-derived microvesicles in tumor progression via a global comparative microvesicular and cellular transcriptomic analysis of human SW480 CRC cells. We first identified 11,327 microvesicular mRNAs involved in tumorigenesis-related processes that reflect the physiology of donor CRC cells. We then found 241 mRNAs enriched in the microvesicles above donor cell levels, of which 27 were involved in cell cycle-related processes. Network analysis revealed that most of the cell cycle-related microvesicle-enriched mRNAs were associated with M-phase activities. The integration of two mRNA datasets showed that these M-phase-related mRNAs were differentially regulated across CRC patients, suggesting their potential roles in tumor progression. Finally, we experimentally verified the network-driven hypothesis by showing a significant increase in proliferation of endothelial cells treated with the microvesicles.</p> <p>Conclusion</p> <p>Our study demonstrates that CRC cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells, suggesting that microvesicles of cancer cells can be involved in tumor growth and metastasis by facilitating angiogenesis-related processes. This information will help elucidate the pathophysiological functions of tumor-derived microvesicles, and aid in the development of cancer diagnostics, including colorectal cancer.</p

Telecommuting-related health outcomes during the COVID-19 pandemic in South Korea: a national population-based cross-sectional study

Background Telecommuting has expanded greatly during the COVID-19 pandemic. Since the advent of remote working from home, there has been an ongoing controversy about the positive or negative health-related impact of telecommuting. This study aimed to investigate change in the occupational health risk in South Korean workers involved in telecommuting during the pandemic period compared to daily commuters. Methods A population-based cross-sectional study of South Korean workers using the secondary data from the 6th Korean Working Conditions Survey (2020–2021) was designed. A total of 12,354 white-collar wage employees were selected as the study sample. Telecommuting, depression, anxiety, insomnia, fatigue, musculoskeletal pain, headache-eye strain, absenteeism, and presenteeism were measured by self-reported data. Multiple logistic regression models, including gender stratification analysis, were used to estimate the adjusted odds ratio (AOR) with a 95% confidence interval (CI) for the health outcomes of telecommuters. Results Among the study population, 338 males and 318 females were reported to be telecommuters. The entirely adjusted regression model showed a positive association between telecommuting and anxiety (AOR = 2.82; 95% CI, 1.93–4.10), insomnia (AOR = 1.93; 95% CI, 1.27–2.92), fatigue (AOR = 1.76; 95% CI, 1.30–2.37), musculoskeletal pain (AOR = 1,76; 95% CI, 1.33–2.32), headache-eye strain (AOR = 1.94; 95% CI, 1.48–2.54), presenteeism (AOR = 1.66; 95% CI, 1.20–2.28) respectively. Gender difference was identified in that only female telecommuters had a higher risk of depression (AOR = 1.62; 95% CI, 1.04–2.53) and insomnia (AOR = 2.07; 95% CI, 1.26–3.41) than daily commuters in the adjusted model. Conclusion Telecommuting was significantly associated with an increased risk of various health problems among South Korean workers and females were identified as a more vulnerable group. Although further research is required to ascertain the causal relationship, public health intervention should be considered to prevent the negative effects of telecommuting.This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number, 2022R1A2C2010463, 2017R1E1A1A01078235). This research was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) and Korea Dementia Research Center (KDRC), funded by the Ministry of Health & Welfare and Ministry of Science and ICT, Republic of Korea (grant number: HU20C0487). This work was supported by the Education and Research Encouragement Fund of Seoul National University Hospital