Euthanasia: Considerations Regarding Depression and Ethics

Articl

Effects of Dietary Cholesterol and its Oxidation Products on Pathological Lesions and Cholesterol and Lipid Oxidation in the Rabbit Liver

The effects of dietary cholesterol (CHO) and cholesterol oxidation productions (COPs) on the induction of pathological lesions in rabbit liver tissues were determined. Rabbits were fed with a chow containing no additives or added with 1 g CHO, 2 g CHO, 0.9 g CHO + 0.1 COPs, 0.8 g CHO + 0.2 g COPs, 0.5 g CHO + 0.5 g COPs, 1.6 g CHO + 0.4 g COPs, or 1.2 g CHO + 0.8 g COPs per kg diet. Liver lesions were induced only when the levels of CHO and COPs in the diet were very high. The amount of CHO in the liver increased when dietary CHO was increased; by comparison, dietary COPs affected liver CHO amounts to a lesser extent. The TBARS (thiobarbituric acid reactive substances) value of the liver samples also increased when dietary CHO and COP levels were elevated, and the TBARS value was more strongly affected by the amount of COPs in the diet than by the amount of CHO. At 6 and 12 weeks, COP levels were highest in the group that received 1.2 g CHO + 0.8 g COPs, followed by the 0.5 g CHO + 0.5 g COPs and 1.6 g CHO + 0.4 g COPs groups; the control (0 g) group showed the lowest COP levels among all groups. This indicated that not only dietary CHO but also COPs were involved in hypercholesterolemia-induced liver lesions when the amount of CHO and COPs was high

Observation of confined propagation in Bragg waveguides

A new type of waveguiding in a slab dielectric bounded on one side by air and on the other by a periodic layered medium (grown by molecular beam epitaxy) has been demonstrated

Optical surface waves in periodic layered media

A generalized analysis of wave propagation in periodic layered media is applied to the special case of optical surface waves. These waves, confined to the interface between a periodic layered medium and a homogeneous medium, are formally analogous to electronic surface states in crystals. Single-mode surface-wave propagation along the surface of a GaAs-AlGaAs multilayer stack (grown by molecular-beam epitaxy) has been observed experimentally

Engineered CHO-K1 by Gene Edition and Its Application to Recombinant Antibody Expression

抗体药物是近年来发展最快的一类生物技术药物，因其极强的靶向特异性，已成功运用于多种疾病的治疗；而目前大部分抗体药物均由中国仓鼠卵巢癌（Chinesehamsterovary，CHO）细胞进行生产。CHO有多个衍生品系，其中CHO-K1在工业中运用较广；CHO-K1主 要有两个品系CHO-K1-CCL-61和CHO-K1-CRL-9618，初始均以血清依赖的贴壁培养方式进行培养，工业生产中通常需要对其进行无血清悬浮适应，甚至对其基因组进行适当的改造；目前CHO-K1-CCL-61的运用和研究较为广泛；而关于CHO-K1-CRL-9618的运用和基础研究均较少。本研究以CHO-K1-CRL-961...Antibody drugs have been one of the most fast-growing biotech drugs in recent years. Because of its high specificity, it has been successfully used in the treatment of many diseases. At present, most of the antibody drugs are produced by the Chinese hamster ovary cancer (CHO) cells. There are a number of CHO derivative lines in which CHO-K1 is widely used in industry. CHO-K1-CCL-61 and CHO-K1-CRL-...学位：理学博士院系专业：生命科学学院_生物化学与分子生物学学号：2162010015390

Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study

Sphingomyelins (SM) and phosphatidylcholines (PC) are major lipid classes in the external plasma membrane leaflet of mammalian cells. A preferential interaction between SM and cholesterol (Cho) in both cell and model membranes has been proposed as central for the formation of Cho- and SM-rich domains in membranes. In this context, the relevance of the SM hydrophobic moiety on its interaction with Cho for domain stabilization has been investigated by our group (1-2). We report here on the effects of sphingomyelin structure on the orientational and conformational properties of monolayers of pure lipids and of two ternary lipid mixtures (DOPC/16:0SM/Cho and DOPC/24:1SM/Cho), which are relevant as mammalian cell membrane models. We investigated interchain interactions, hydrogen bonding, conformational and structural properties using in situ polarization modulated infrared reflection absorption spectroscopy (PM-IRRAS). Our results indicate that the particular properties conferred on sphingolipids by unsaturation have profound implications on membrane organization.Finally, we also explored the orientational and conformational changes in lipid monolayers of DOPC/16:0SM/Cho 2:1:1 after the adsorption/insertion of the active toxin HlyA and its unacylated nonhemolytic precursor ProHlyA, so as to complement our knowledge on the action mechanism of both proteins.Instituto de Investigaciones Bioquímicas de La PlataComisión de Investigaciones Científicas de la provincia de Buenos AiresInstituto de Investigaciones Fisicoquímicas Teóricas y AplicadasCentro de Investigación de Proteínas Vegetale

Assessment of metal exposure (uranium and copper) in fatty acids and carbohydrates profiles of Calamoceras marsupus larvae (Trichoptera) and Alnus glutinosa

Physiological changes were explored in fatty acids (FA) and carbohydrate (CHO) composition in the shredder Calamoceras marsupus larvae (Trichoptera) and leaf litter (C. marsupus food) exposed to copper and uranium under natural and experimental conditions. We measured FA and CHO content in leaf litter and larvae specimens from reference and impacted streams, and exposed for 5 weeks to four realistic environmental concentrations of copper (35 μg L−1 and 70 μg L−1) and uranium (25 μg L−1 and 50 μg L−1). Regarding FA, (1) leaf litter had a reduced polyunsaturated FA (PUFA) content in metal treatments, s (14 to 33% of total FA), compared to natural conditions (≥39% of total FA). Leaf litter exposed to uranium also differed in saturated FA (SFA) composition, with lower values in natural conditions and higher values under low uranium concentrations. (2) C. marsupus had/showed low PUFA content under Cu and U exposure, particularly in high uranium concentrations. Detritivores also decreased in PUFA under exposure to both metals, particularly in high uranium concentrations. On the other hand, (1) microorganisms of the biofilm colonizing leaf litter differed in CHO composition between natural (impacted and reference) and experimental conditions, with glucose and galactose being consistently the most abundant sugars, found in different amounts under copper or uranium exposure; (2) CHO of detritivores showed similar high galactose and fucose concentrations in contaminated streams and high copper treatments, whereas low copper treatment showed distinct CHO profiles, with higher mannose, glucose, arabinose, and fucose concentrations. Our study provides evidence of metal exposure effects on FA and CHO contents at different trophic levels, which might alter the quality of food flow in trophic websFil: Tagliaferro, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Rocha, Carolina. Universidad de Coimbra. Facultad de Ciencias E Tecnología. Departamento de Ciencias Da Vida; PortugalFil: Marques, Joao Carlos. Universidad de Coimbra. Facultad de Ciencias E Tecnología. Departamento de Ciencias Da Vida; Portugal. Marine And Environmental Sciences Centre; PortugalFil: Goncalves, Ana Marta. Universidad de Coimbra. Facultad de Ciencias E Tecnología. Departamento de Ciencias Da Vida; Portugal. Marine And Environmental Sciences Centre; PortugalVIII Congreso Argentino de la Sociedad de Toxicología y Química AmbientalMar del PlataArgentinaSociedad de Toxicología y Química Ambienta

FAKTOR-FAKTOR YANG MEMPENGARUHI KINERJA COMPLAINT HANDLING OFFICER (CHO) DI UNIT KERJA CALL CENTER TELKOMSEL MAKASSAR

  Abstract Complaint Handling Officer (CHO) must perform according to the Key Performance Indicator (KPI) set by the company and to this day the Telkomsel Makassar Call Center CHO can successfully achieve the set targets. The purpose of this study is to determine the factors that influence the performance of the Complaint Handling Officer in the Telkomsel Call Center work unit. The research method used is quantitative associative method. This method is used to test whether there is influence fromPersonal Factor, Leadership Factor, Team Factor, System Factor, Contextual /  Situational Factor to Performance Complaint Handling Officer at Call Center Unit Telkomsel Makassar. Methods of data analysis of this study are applying normality test, regression analysis, and hypothesis test with the help of SPSS version 21. Partial research results, the relationship of personal factors have no positive and significant effect on the performance of CHO with probability value is 0.592. leadership factors do not have a positive and significant effect on the performance of CHO with a probability value of 0.910. there is a positive and significant influence of team factor on CHO performance with probability value is 0,014. system factor has no positive and significant effect on performance with probability value that is 0,650. there is no positive and significant influence of environmental factor on CHO performance with probability value is 0,878. Simultaneously (F test) all independent variables are personal, leadership, team, system, contextual / situational no significant and significant to CHO performance, with probabiltas value (sign) 0.052. It can be said that personal, leadership, team, system and contextual / situational factors together do not affect CHO performance.  Abstrak Petugas Complaint Handling Officer (CHO) harus memiliki kinerja sesuai  Key Performance Indicator (KPI) yang telah ditetapkan oleh perusahaan dan sampai saat ini CHO Call Center Telkomsel Makassar bisa berhasil mencapai target - target yang telah ditetapkan tersebut. Adapun tujuan dari penelitian ini adalah untuk mengetahui Faktor-faktor yang mempengaruhi Kinerja Complaint Handling Officer pada Unit kerja Call Center Telkomsel Makassar. Metode penelitian yang digunakan adalah  metode kuantitatif assosiatif, metode ini digunakan untuk menguji ada tidaknya pengaruh dari  personal factor, leadership  factor, team factor, system factor, contextual/situasional factor  terhadap kinerja complaint handling officer pada unit kerja Call Center Telkomsel Makassar. Metode analisis data penelitian ini menggunakan uji normalitas, analisis regresi, dan uji hipotesis dengan bantuan SPSS versi 21. Hasil penelitian secara parsial, hubungan faktor personal tidak memiliki pengaruh positif dan signifikan terhadap kinerja CHO dengan nilai probabilitasnya yaitu 0,592. Faktor kepemimpinan tidak berpengaruh positif dan signifikan terhadap kinerja CHO dengan nilai probabilitas yaitu 0,910. ada pengaruh positif dan signifikan dari faktor team terhadap kinerja CHO dengan nilai probabilitasnya yaitu 0,014. Faktor sistem tidak berpengaruh positif dan signifikan terhadap kinerja dengan nilai probabilitasnya yaitu 0,650. Tidak ada pengaruh positif dan signifikan dari faktor lingkungan  terhadap kinerja CHO dengan nilai probabilitasnya yaitu 0,878. Secara simultan (uji F) semua variabel bebas yaitu personal, leadership, team, system, kontextual/situasional tidak berpengaruh dan signifikan terhadap kinerja CHO,  dengan nilai probabiltas (sign) 0,052. Dapat dikatakan bahwa faktor personal, leadership, team system dan contextual/situasional secara bersama – sama tidak berpengaruh terhadap kinerja CHO. &nbsp