Effect of grafting on growth and incidence of phytophthora blight and bacterial wilt of pepper (Capsicum annuum L.)

This study was conducted to investigate the effect of grafting using commercial rootstocks and breeding lines on growth and resistance to both Phytophthora blight (Phytophthora capsici) and bacterial wilt (Ralstonia solanacearum), and to evaluate the breeding lines as candidates rootstocks for grafted peppers. Capsicum annuum 'Nokkwang' (scion) was grafted onto five commercial rootstocks ('Kataguruma', 'Konesianhot', 'Koregon PR-380', 'PR-power', and 'Tantan') and nine breeding lines ('PR 901', 'PR 919', 'PR 920', 'PR 921', 'PR 922', 'PR 927', 'PR 928', 'PR 929', and 'PR 930'). Graft-take percentage was over 80% in all the grafted seedlings except those grafted onto 'PR 901' (75%). The number of leaves, stem diameter, leaf area, and dry weight of shoot of grafted seedlings were greater than those of non-grafted seedlings. Mineral contents in the shoots of grafted seedlings were significantly different, depending on rootstock genotypes. Marketable yield ranged from 1.35 to 1.96 kg per plant depending on rootstock genotypes, and was highest in peppers grafted onto 'PR 901' (1.95 kg) and non-grafted peppers (1.96 kg), and lowest in those grafted onto 'PR 928' (1.35 kg). Gross yield was highest in peppers grafted onto 'Konesianhot' (2.64 kg). Among nine breeding lines, three rootstocks ('PR 920', 'PR 921', and 'PR 922') were selected as candidate rootstocks for the grafted pepper based on graft-take, growth, yield, fruit quality, and resistance to diseases. Three major pepper cultivars ('Nokkwang', 'Saengsaeng Matkkwari', and 'Shinhong') were grafted onto those three breeding lines and 'Tantan' (control). Peppers grafted onto breeding lines of 'PR 920', 'PR 921', and 'PR 922' showed greater resistance to both Phytophthora blight and bacterial wilt without the decrease in yield and fruit quality. Accordingly, they were considered to be used as rootstocks resistant to both Phytophthora blight and bacterial wilt for pepper production.OAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000027607/1SEQ:1PERF_CD:SNU2012-01EVAL_ITEM_CD:102USER_ID:0000027607ADJUST_YN:YEMP_ID:A075898DEPT_CD:517CITE_RATE:0FILENAME:2012-2-heb-접목-내병성-장윤아.pdfDEPT_NM:식물생산과학부EMAIL:[email protected]_YN:NCONFIRM:

Genome-wide identification and expression analysis of CaLAX and CaPIN gene families in pepper (Capsicum annuum L.) under various abiotic stresses and hormones treatments

Auxin plays key roles in regulating plant growth and development as well as in responses to environmental stresses. The intercellular transport of auxin is mediated by four gene families, with the ATP-binding cassette family B (ABCB), the Auxin resistant1/ like (AUX1AUX/LAX) family, the pin-formedďź PINďź families, and the PIN-LIKES (PILS) family. Here, the latest assembled pepper genome was used to characterise and analyse the CaLAX and CaPIN families. Genome-wide investigations into these families, including chromosomal distributions, phytogenic relationships and intron/exon structures, were performed. In total, 4 CaLAXs and 10 CaPINs were mapped to 10 chromosomes. Most of these genes exhibited varied tissue-specific expression patterns assessed by quantitative real-time PCR. The expression profiles of the CaLAX and CaPIN genes under various abiotic stresses (salt, drought, and cold), exogenous phytohormones (IAA, 6-BA, ABA, SA and MeJA) and polar auxin transport inhibitors treatments were evaluated. Most CaLAX and CaPIN genes were altered by abiotic stress at the transcriptional level in both shoots and roots, and many CaLAX and CaPIN genes were regulated by exogenous phytohormones. Our study helps to identify candidate auxin transporter genes and to further analyse their biological functions in pepper development and in its adaptation to environmental stresses.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author