Fabrication of nano-mosquitocides using chitosan from crab shells: impact on non-target organisms in the aquatic environment

Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a novel method of biofabrication of silver nanoparticles (AgNP) using chitosan (Ch) from crab shells. Ch-AgNP nanocomposite was characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. Ch-AgNP were tested against larvae and pupae of the malaria vector Anopheles stephensi obtaining LC50 ranging from 3.18 ppm (I) to 6.54 ppm (pupae). The antibacterial properties of Ch-AgNP were proved against Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi, while no growth inhibition was reported in assays conducted on Proteus vulgaris. Concerning non-target effects, in standard laboratory considtions the predation efficiency of Danio rerio zebrafishes was 68.8% and 61.6% against I and II instar larvae of A. stephensi, respectively. In a Ch-AgNP-contaminated environment, fish predation was boosted to 89.5% and 77.3%, respectively. Quantitative analysis of antioxidant enzymes SOD, CAT and LPO from hepatopancreas of fresh water crabs Paratelphusa hydrodromous exposed for 16 days to a Ch-AgNP-contaminated aquatic environment were conducted. Notably, deleterious effects of Ch-AgNP contaminating aquatic enviroment on the non-target crab P. hydrodromous were observed, particularly when doses higher than 8–10 ppm are tested. Overall, this research highlights the potential of Ch-AGNP for the development of newer control tools against young instar populations of malaria mosquitoes, also highlighting some risks concerned the employ of nanoparticles in aquatic environments

Effects of seepage flow on liquefaction resistance of uniform sand and gap-graded soil under undrained cyclic torsional shear

Internal erosion is the transportation of soil particles from within or beneath geotechnical structures, caused by seepage flow, that impacts the subsequent mechanical and hydraulic behaviour of the soil. However, it is difficult to predict the liquefaction resistance of eroded soil due to several factors related to the soil fabric. The present study investigates the impact of seepage flow on the undrained cyclic behaviour of two types of soil: uniform sand and gap-graded soil with a fines content of 20%, using a novel erosion hollow cylindrical torsion shear apparatus. From the results for the uniform sand, the soil fabric formed by moist tamping (MT) leads to higher liquefaction resistance than that formed by air-pluviation (AP). However, after applying seepage flow, the liquefaction resistance of the eroded MT specimens becomes even lower than that of the non-eroded AP specimen. Therefore, the liquefaction resistance of soil is expected to decrease due to the rearrangement of the initially stable coarse particles during seepage flow. On the other hand, the liquefaction resistance of the gap-graded soil tends to increase after the removal of fines as the number of stable contacts between the coarse particles is increased. Under these test conditions, the latter effect is found to be greater for the given gradation, leading to a slight increase in the liquefaction resistance of the tested gap-graded soil after internal erosion. Furthermore, the intergranular void ratio and small-strain shear modulus are seen to be well correlated with the liquefaction resistance of the tested soil

Impact of core polarity on smectic B-induced hydrogen bond liquid crystals

The novel series of hydrogen bond liquid crystals were synthesized from the 2-methylglutaric acid (MGA) and 4-alkyloxybenzoic acid (nOBA) compounds. The induced smectic B phase with different texture (spine texture, needle texture, mosaic texture, natural mosaic texture and marble texture) were identified by polarizing optical microscope. Due to breaking of in-plane rotational symmetry within molecular layers, smectic B phase is tempted by suppressing other usual mesophases. The mesomorphic transition temperature, enthalpy and entropy values were calculated by differential scanning calorimeter which strongly proves the existence of mesomorphism. H-bond interaction and functional groups were confirmed by the observed peak between 2910 and 2954 cm−1 in the FTIR spectra. Thermal stability and extended mesophase width (for MGA + 12OBA = 31.1) of Sm B mesophase were reported and it clearly reveals the existence of mono-phase variance in the MGA + nOBA HBLC complex. Due to the steric effect, and the increased molecular core polarity, the highly stabilized Sm B phase with different textures were observed while varying alkyloxy carbon number n = 7 to 12. Further, the origination of Sm B phase and its detailed characteristics were reported