ANTIMICROBIAL SUSCEPTIBILITY PROFILE OF MICROBIAL PATHOGENS ISOLATED FROM CALVES WITH RESPIRATORY DISEASES

Introduction: Respiratory disease in calves is an actual problem, a major cause of economic losses due to mortality, growth delay and improper development. These conditions are frequent in calves due to the weaning stress, transport and environmental changes.Aims: The aim of this study was the isolation of bacteria from 30 calves with respiratory disorders and their antibiotic susceptibility testing.Materials and methods: Samples were collected from calves with respiratory disorders (nasal discharge) aged 6 to 9 weeks in 2 series, using sterile swabs. Samples were initially inoculated on blood agar and MacConkey agar following the characteristics of the colonies and microscopic examination that enabled the identification of bacterial species. Isolated strains were used to flood Mueller-Hinton agar to carry out sensitivity testing. The antibiotics tested were represented by: Amoxicillin with clavulanic acid, Gentamicin, Florfenicol, Enrofloxacin, Marbofloxacin, Penicillin G, Cefquinone, Tulathromycin, Ceftiofur, Tylosin and Cephalotin.Results: Genus Streptococcus have been identified in 23 samples, followed by Staphylococcus identified in 14 samples, and Bacillus spp., in 10 nasal swabs; The most common bacteria associations were represented by Streptococcus-Staphylococcus, Streptococcus-Staphylococcus-Bacillus, and Streptococcus-E.coli. The most efficient antibiotic was Cefquinome (Cobactan), followed by Penicillin G and Amoxicillin with clavulanic acid (Amoxiclav); the least effective antibiotics were Florfenicol and Tulathromycin. Conclusions: The study carried out on nasal discharge samples collected from calves with respiratory disorders and their antimicrobial profile testing led to the following conclusions: 1) Low susceptibility to Florfenicol is caused by previous treatments when this molecule was excessively used and without prior sensitivity testing. 2) Cefquinome may represent an emergency therapeutic antibiotic for respiratory infections in calves, but the administration should always be preceded by susceptibility testing of the isolates

Investigation on the Incidence, Diagnostic, Possibilities and Design of Prophylactic Treatment in an Outbreak of Bacterial Haemorrhagic Septicemia of Carp

The investigations carried out on a sample of 53 breeders (31 females and 22 males), in a fishing farm of Suatu village, County of Cluj, concerning the incidence of hemorrhagic bacterial septicaemia, diagnosis and profilactico-therapeutic possibilities. Results highlights a incidence of bacteriosis differentiated according to the category of breeders, 54.8% in females and 36.3% in males. Necropsy examination carried out on 3 specimen of suspected bacteriosis, highlights the presence of hemorrhagic skin lesions, which are dotted or diffuse, respectively musculo-cutaneous necrosis and ulcers. Regarding the internal injuries, opening of the body cavity, revealed consistency changes and septicemy form in major internal organs, as well as the presence of a liquid hollow looking sanguinolent.The bacterioscopic examination of smears from the pathologic material (skin surface end internal samples) and colored with the Gram method, reveled bacterial microflora, represented by coccobacillus and straight bacilli or slightly curved, both Gram negative. Furthermore, the bacteriological (culture) exam performed on usual and selective culture mediae, distinguishes the isolation of pure culture bacteriae belonging to the genus Aeromonas. The exam of biochemical traits, with the aid of API 20 E multitest determines that the isolated strains of Aeromonas spp. belong to the species Aeromonas hydrophila, indicted in the emergence and evolution of the bacterial of haemorrhagic septicaemia. Testing strains sensitivity to antibiotics and chemotherapy (through antibiograma level), reflects the isolated bacteria’s values of sensitivity in decreasing order: amoxicillin, oxytetracycline, florfenicol and enrofloxacilin. However, reduced sensitivity was recorded to tetracycline and ampicillin and resistance to erythromycin.Â

Mastitis Acuta in a Pure Breed Cane Corso female. A Case Report

Data regarding bitch mastitis is fairly scarce compared to the literature regarding ruminant mastitis. Neglecting clinical and subclinical cases of mastitis can be life threatening for both dam and puppies. The aim of this report is to present a case of Mastitis acuta complicated with a case of neonatal septicaemia in a Cane Corso (Canis lupus familiaris L.) pure breed female. Laboratory assays showed a milk pH value of 7.5, milk cytology revealed the presence of segmented neutrophils, while foamy cells, phagocytosis and highly pathogenic bacteria (i.e. Escherichia coli and Pseudomonas aeruginosa) were isolated from milk and puppy. To the author's knowledge, this is the first case of bitch mastitis linked to neonatal septicaemia with mixed E. coli and P. aeruginosa infection