1 research outputs found
Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells
Objective: Cellular damage induced by oxidative stress has been involved in the development of neurodegeneration.
Curcumin, a dietary polyphenol found in the rhizome of Curcuma longa, has been shown, both in vitro and in vivo,
to be an effective reactive oxygen scavenging molecule. We investigated an anti-oxidative effect of curcumin against
H2
O2
-induced toxicity in human neuroblastoma cell line SK-N-SH.
Methods: The SK-N-SH cells were pre-treated with curcumin 2 hours prior to H2
O2
treatment. We measured cell
viability, intracellular reactive oxygen species (ROS) levels, expression of apoptotic-related proteins and caspase-3
activity 24 hours post H2
O2
-induced cytoxicity.
Results: Treatment with curcumin at concentrations ranging from 5 to 50 μg/mL was not cytotoxic. Pre-treatment
with curcumin at the concentrations of 5 to 50 μg/mL prior to H2
O2
exposure caused a significant decrease in
intracellular ROS levels and a significant increase in cell viability in a dose-dependent manner. Expression of activated
form of caspase-3 and BAX, a pro-apoptotic protein, measured by Western blotting were reduced when the SK-N-
SH cell line was pre-incubated with curcumin. The curcumin pre-treated cells also exhibited less caspase-3 activity.
Conclusion: Curcumin has protective effects against H2
O2
-induced toxicity in a dose-dependent manner through
its anti-apoptotic and anti-oxidative properties in an in vitro H2
O2
-treated SK-N-SH model