RANCANG BANGUN APLIKASI PEMANDU WISATA DANAU TOBA BERBASIS WEB MENGGUNAKAN METODE AGILE

ABSTRACT Lake Toba is the largest lake in Indonesia and the largest volcanic lake in the world, which is currently one of the tourist destinations in North Sumatra. Lake Toba has many tourist objects that are very beautiful and unspoiled, which will give satisfaction to every visitor. However, due to the lack of application of information technology, it often limits the number of tourists who will visit. This research was made to make it easier for tourists when visiting Lake Toba by creating an application to guide tourists when traveling on Lake Toba. Researchers make a web-based application, using the Agile method as a system development and using mysql as a database in order to further increase tourist satisfaction using the application. The results of this study are the application of a Lake Toba tour guide that will provide convenience and satisfaction in traveling on Lake Toba. This application will provide instructions for every tourist spot on Lake Toba, complete with routes to access these attractions. Application users can also provide ratings of attractions that have been visited, along with comments about experiences or suggestions for attractions. This application will also display the estimated fare to enter tourist destinations and transportation that will be used by tourists. This application will be accessible if connected to the internet network

Knowledge of Critical Care Provider on Prevention of Ventilator Associated Pneumonia

Background: Ventilator-associated pneumonia (VAP) continues to be an important cause of morbidity and mortality in ventilated patient. Prevention of VAP in critically ill patient is significant concern for health care team in intensive care units (ICUs). Knowledge on prevention of VAP would have a significant impact on patient outcome. Aims and Objectives: To assess knowledge on prevention of VAP in critical care providers and to find the association between knowledge on prevention of VAP and educational qualification and years of experience in ICUs. Settings and Design: The study was conducted in 5 different ICUs of Kasturba Hospital, Manipal, and using descriptive study design. Material and Methods: The study involved a purposive sample of 138 critical care providers. Critical care providers who were willing to participate in the study were included. Tools on demographic proforma and self-administered structured knowledge questionnaire on prevention of VAP were developed and content validity was established. The reliability of the tools was established.The data was categorized and analyzed by using descriptive and inferential statistics. The SPSS 16.0 version was used for the analysis of the study. Result: Majority 89.1% of the participant were 20-29 years, 63% unmarried 51.4% had completed diploma course and majority 81.2% were from nursing discipline. The study revealed that only 55.80% of subjects were having adequate knowledge on prevention of VAP based on median score. There was no significant association between knowledge score and educational qualification (÷²=0, p=0.833), years of experience in ICU (÷²= 2.221, p=0.329)

Machbarkeitsstudie Campus Puttkamer- / Wilhelm- / Kochstraße

MACHBARKEITSSTUDIE CAMPUS PUTTKAMER- / WILHELM- / KOCHSTRASSE Machbarkeitsstudie Campus Puttkamer- / Wilhelm- / Kochstraße / Richter, Andreas (Rights reserved) ( -

Kesantunan Berbahasa Di Kalangan Remaja Desa Purwosari Kelurahan Jurangjero Kecamatan Karangmalang Kabupaten Sragen

This study has two objectives, namely: (1) Describe the form of politeness in the language of Purwosari village, Jurangjero village, Karangmalang district, Sragen regency. (2) Describe the principle of politeness in language in Purwosari village, Jurangjero village, Karangmalang district, Sragen regency. (3) Describe the language politeness scale spoken by the community in Purwosari village, Jurangjero village, Karangmalang district, Sragen regency. This type of research is descriptive qualitative. The data source in this study is oral data taken at the Purwosari village. This data collection technique is done by recording technique, which is a data collection teachnique used by recording informant conversations, especially those related to the problem under study. Data analysis method used is the equilent method. The basic technique referred to “the choice element determinant or PUP technique. Data analysis in this research has been done since the process of collecting data and for the validity of the data techniques in this study using the theory trianggulation technique. Based on the analysis of language politeness data in Purwosari village, Jurangjero sub-district, Karangmalang subdistrict, Sragen regency, there are 9 data on the principle of politeness in language spoken by teenagers, namely: (a) speech containing maxim of wisdom 2 utterances, (b) utterance containing maxim of acceptance of 1 utterance, (c) utterance containing maxim of generosity of 1 utterance, (d) utterance which contains maxims of humility 1 utterance, (e) utterances that contain a maximal compatibility of 1 utterance, and (f) utterances that contain a maximal sympathy of 3 utterances. There are five language politeness scales in the speech of adolescent children, namely (a) speech which contains a profit and loss scale of 1 speech, (b) speech containing a choice of 1 speech scale, (c) speech containing a continuation scale of 1 speech, (d) utterances that contain an authoritative scale of 1 utterance, (e) utterances containing a social distance scale of 1 utterance. Keywords: Politeness in language, Youth, Villag

Extent of N-terminus exposure of monomeric alpha-synuclein determines its aggregation propensity

Abstract: As an intrinsically disordered protein, monomeric alpha-synuclein (aSyn) occupies a large conformational space. Certain conformations lead to aggregation prone and non-aggregation prone intermediates, but identifying these within the dynamic ensemble of monomeric conformations is difficult. Herein, we used the biologically relevant calcium ion to investigate the conformation of monomeric aSyn in relation to its aggregation propensity. We observe that the more exposed the N-terminus and the beginning of the NAC region of aSyn are, the more aggregation prone monomeric aSyn conformations become. Solvent exposure of the N-terminus of aSyn occurs upon release of C-terminus interactions when calcium binds, but the level of exposure and aSyn’s aggregation propensity is sequence and post translational modification dependent. Identifying aggregation prone conformations of monomeric aSyn and the environmental conditions they form under will allow us to design new therapeutics targeted to the monomeric protein

Extent of N-terminus exposure of monomeric alpha-synuclein determines its aggregation propensity

Abstract: As an intrinsically disordered protein, monomeric alpha-synuclein (aSyn) occupies a large conformational space. Certain conformations lead to aggregation prone and non-aggregation prone intermediates, but identifying these within the dynamic ensemble of monomeric conformations is difficult. Herein, we used the biologically relevant calcium ion to investigate the conformation of monomeric aSyn in relation to its aggregation propensity. We observe that the more exposed the N-terminus and the beginning of the NAC region of aSyn are, the more aggregation prone monomeric aSyn conformations become. Solvent exposure of the N-terminus of aSyn occurs upon release of C-terminus interactions when calcium binds, but the level of exposure and aSyn’s aggregation propensity is sequence and post translational modification dependent. Identifying aggregation prone conformations of monomeric aSyn and the environmental conditions they form under will allow us to design new therapeutics targeted to the monomeric protein

Phosphorylation of huntingtin at residue T3 is decreased in Huntington’s disease and modulates mutant huntingtin protein conformation

Posttranslational modifications can have profound effects on the biological and biophysical properties of proteins associated with misfolding and aggregation. However, their detection and quantification in clinical samples and an understanding of the mechanisms underlying the pathological properties of misfolding- and aggregation-prone proteins remain a challenge for diagnostics and therapeutics development. We have applied an ultrasensitive immunoassay platform to develop and validate a quantitative assay for detecting a posttranslational modification (phosphorylation at residue T3) of a protein associated with polyglutamine repeat expansion, namely Huntingtin, and characterized its presence in a variety of preclinical and clinical samples. We find that T3 phosphorylation is greatly reduced in samples from Huntington\u2019s disease models and in Huntington\u2019s disease patients, and we provide evidence that bona-fide T3 phosphorylation alters Huntingtin exon 1 protein conformation and aggregation properties. These findings have significant implications for both mechanisms of disease pathogenesis and the development of therapeutics and diagnostics for Huntington\u2019s disease

Development of novel methods and tools to decipher the huntingtin post-translation modifications code

Huntington's disease (HD) is a fatal genetic neurodegenerative disorder caused by a CAG repeat expansion in the Huntingtin gene of more than 36 repeats. This repeat is translated into a polyglutamine (polyQ) stretch within the first exon-encoded region of the Huntingtin protein (Httex1). The aggregation of expanded Huntingtin protein (Htt) in the striatum and cortex has been implicated in neuronal dysfunction and death. Recent studies have shown that mutations that mimic post-translational modifications (PTMs) in the first 17 N-terminal amino acids (Nt17) of the protein dramatically influence the aggregation, subcellular localization, and toxicity of Httex1 and full-length Htt proteins. These findings suggest that these modifications may serve as reversible molecular switches for regulating Htt function in health and disease. However, assessing the effect of authentic PTMs and their cross-talk has not been possible due to the lack of knowledge about the enzymes responsible for these modifications. Moreover, the high aggregation propensity of Httex1 with increased polyQ content hampered the development of an efficient method to produce post-translationally modified proteins. By consequence, previous biophysical studies were mostly carried out using tagged Httex1 protein, or model peptides bearing PTM mimetics mutations which do not fully capture the chemical properties of the bona fide PTMs. Therefore, we developed a semisynthetic methodology that permits site-specific modification of the mutant Httex1 (at single or multiple residues). We used this strategy to investigate the effect of acetylation (at K6, K9, or K15) and phosphorylation (at T3), as well as the cross-talk between these modifications on the structure and aggregation of wild-type and mutant Httex1. Our results demonstrated that T3 phosphorylation (pT3) significantly inhibited the aggregation of mutant Httex1 (43Q), whereas only partial inhibition of aggregation was achieved by the phosphomimetic mutation (T3D). Acetylation of single ly-sine residues, K6, K9, or K15 (AcK6, AcK9, or AcK15), did not affect the aggregation of Httex1. Interestingly, AcK6, but not at AcK9 or K15, reversed the inhibitory effect of pT3. Next, we discovered novel kinases GCK and TBK1 that phosphorylate efficiently and specifically T3 and S13/S16, respectively. We exploited these kinases to produce homogenously phosphorylated recombinant Httex1 at T3 or both S13 and S16. We generated, using this method, suitable for structural and cellular applications. Finally, we combined chemical, semisynthetic and enzymatic methods to produce mutant Httex1 proteins bearing oxidized Methionine at position 8 (oxM8) alone or in combination with other Nt17 PTMs (pT3 or AcK6). We ob-served that oxM8 delayed the aggregation of mutant Httex1 and decreased the helical content of the Nt17 independently of the presence of other PTMs. Together, these findings provide novel insight into the role of Nt17 PTMs in regulating the aggregation of Httex1 and suggest that the aggregation and possibly the function (s) of Httex1 are controlled by complex regulatory mechanisms involving cross-talk between different PTMs. The tools presented in this thesis will open new windows of opportunity to decipher the role of Htt PTMs code in the regulation of Htt function in health and disease

Elaboración de recursos didácticos para el mejoramiento de los procesos de enseñanza aprendizaje en el área de lenguaje y literatura para los niños del primer año de Educación General Básica de la escuela "Atilio Ampam" cantón Morona, periodo lectivo 2011-2012

La destrucción sistemática del lenguaje, del uso correcto de la palabra, es un grave signo de alienación futura. La lengua y la literatura son puertas que nos abren hacia nuestro interior, y hacia los demás. Al tener deficiencias metodológicas y pedagógicas significantes, en el proceso de enseñanza-aprendizaje de esta temática educativa, mi persona ha emprendido buscar la solución estratégica con la elaboración de recursos didácticos para el mejoramiento de los procesos de enseñanza aprendizaje en el área de lenguaje y literatura para los niños del primer año de educación general básica de la escuela "Atilio Ampam" del Cantón Morona, para el periodo 2011-2012, con una visión viable para una praxis real a través de un empoderamiento personalizado de los educandos locales, con recursos, insumos y sabidurías etno culturales del medio.The systematic destruction of language, the correct use of the word, is a serious sign of future alienation. The language and literature are doors that open into ourselves and to others. By having significant methodological and pedagogical deficiencies in the teaching-learning process of this educational theme, my person has taken to find a solution with the development strategy of teaching resources for the improvement of teaching and learning processes in the area of language arts for children in the first year of basic general education school "Atilio Ampam" Morona Canton for the period 2011-2012, with a viable vision for a real praxis through personal empowerment of local learners, resourceful, ethno cultural inputs and environmental wisdom