Analisi dinamiche in tensioni efficaci con il codice "SCOSSA"

L’incremento di pressioni interstiziali indotto da azioni sismiche in depositi sabbio-limosi determina la progressiva riduzione delle tensioni efficaci e di conseguenza della rigidezza e della resistenza dei terreni, fino a condurre alla completa liquefazione. Un metodo sviluppato per la stima di tale incremento si basa su un parametro semi-empirico, detto ‘parametro di danno’, che consente di applicare direttamente i risultati di prove cicliche di laboratorio per predire l’incremento di pressione interstiziale generato in sito dall’azione sismica. Tale procedura, implementata all’interno di un codice di calcolo non lineare, consente di effettuare analisi monodimensionali in tensioni efficaci senza adottare sofisticati modelli costitutivi del terreno. L’applicazione ad un argine danneggiato dal terremoto emiliano del 2012 ha consentito di evidenziare fenomeni di degradazione ciclica degli strati sabbiosi profondi che non sarebbe stato possibile osservare con le tradizionali analisi in tensioni totali

Un modello semplificato per la previsione dell'incremento di pressione interstiziale indotto da carichi ciclici

Le procedure che consentono la valutazione dell’incremento di pressione interstiziale indotto da azioni sismiche in terreni sabbiosi saturi si basano, tipicamente, sui risultati di prove cicliche di laboratorio a tensione o deformazione controllata. In tali procedure la storia di carico irregolare a cui è sottoposto in terreno in sito deve essere semplificata con una azione equivalente tale che produca, su provini di terreno sottoposti a prove cicliche di laboratorio, lo stesso incremento delle pressioni interstiziali atteso nel sito. In alternativa, nel caso di propagazione monodimensionale, è possibile utilizzare un unico parametro, detto ‘parametro di danno’, per modellare gli incrementi di pressione interstiziale misurati in laboratorio. Questo parametro consente di applicare direttamente i risultati di laboratorio per predire l’incremento di pressione interstiziale generato in sito da storie irregolari di tensione o deformazione dovute all’azione sismica. Tale procedura, per la sua semplicità di applicazione, consente di effettuare analisi della risposta dinamica di depositi sabbiosi saturi senza adottare sofisticati modelli costitutivi del terreno e pertanto si presta all’implementazione in codici di calcolo che operano nel dominio del tempo

List of requirements on formalisms and selection of appropriate tools

This deliverable reports on the activities for the set-up of the modelling environments for the evaluation activities of WP5. To this objective, it reports on the identified modelling peculiarities of the electric power infrastructure and the information infrastructures and of their interdependencies, recalls the tools that have been considered and concentrates on the tools that are, and will be, used in the project: DrawNET, DEEM and EPSys which have been developed before and during the project by the partners, and M\uf6bius and PRISM, developed respectively at the University of Illinois at Urbana Champaign and at the University of Birmingham (and recently at the University of Oxford)

Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.

Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype with 71–82 chromosomes. By comparing 3AB-OS CSCs to the parental cells, array CGH, Affymetrix microarray, and TaqMan1 Human MicroRNA array analyses identified 49 copy number variations (CNV), 3,512 dysregulated genes and 189 differentially expressed miRNAs. Some of the chromosomal abnormalities and mRNA/miRNA expression profiles appeared to be congruent with those reported in human osteosarcomas. Bioinformatic analyses selected 196 genes and 46 anticorrelated miRNAs involved in carcinogenesis and stemness. For the first time, a predictive network is also described for two miRNA family (let-7/98 and miR-29a,b,c) and their anticorrelated mRNAs (MSTN, CCND2, Lin28B, MEST, HMGA2, and GHR), which may represent new biomarkers for osteosarcoma and may pave the way for the identification of new potential therapeutic targets

Methodologies synthesis

This deliverable deals with the modelling and analysis of interdependencies between critical infrastructures, focussing attention on two interdependent infrastructures studied in the context of CRUTIAL: the electric power infrastructure and the information infrastructures supporting management, control and maintenance functionality. The main objectives are: 1) investigate the main challenges to be addressed for the analysis and modelling of interdependencies, 2) review the modelling methodologies and tools that can be used to address these challenges and support the evaluation of the impact of interdependencies on the dependability and resilience of the service delivered to the users, and 3) present the preliminary directions investigated so far by the CRUTIAL consortium for describing and modelling interdependencies

Site response analyses for complex geological and morphological conditions: relevant case-histories from 3rd level seismic microzonation in Central Italy

The paper presents the results of 5 case studies on complex site e ects selected within the project for the level 3 seismic microzonation of several municipalities of Central Italy dam- aged by the 2016 seismic sequence. The case studies are characterized by di erent geo- logical and morphological con gurations: Monte San Martino is located along a hill slope, Montedinove and Arquata del Tronto villages are located at ridge top whereas Capitignano and Norcia lie in correspondence of sediment- lled valleys. Peculiarities of the sites are constituted by the presence of weathered/jointed rock mass, fault zone, shear wave veloc- ity inversion, complex surface and buried morphologies. These factors make the de ni- tion of the subsoil model and the evaluation of the local response particularly complex and di cult to ascertain. For each site, after the discussion of the subsoil model, the results of site response numerical analyses are presented in terms of ampli cation factors and acceleration response spectra in selected points. The physical phenomena governing the site response have also been investigated at each site by comparing 1D and 2D numerical analyses. Implications are deduced for seismic microzonation studies in similar geological and morphological conditions.Published5741–57775T. Sismologia, geofisica e geologia per l'ingegneria sismicaJCR Journa

Cancer cell growth and survival as a system-level property sustained by enhanced glycolysis and mitochondrial metabolic remodeling

Systems Biology holds that complex cellular functions are generated as system level properties endowed with robustness, each involving large networks of molecular determinants, generally identified by "omics" analyses. In this paper we describe four basic cancer cell properties that can easily be investigated in vitro: enhanced proliferation, evasion from apoptosis, genomic instability, and inability to undergo oncogene-induced senescence. Focusing our analysis on a K-ras dependent transformation system, we show that enhanced proliferation and evasion from apoptosis are closely linked, and present findings that indicate how a large metabolic remodeling sustains the enhanced growth ability. Network analysis of transcriptional profiling gives the first indication on this remodeling, further supported by biochemical investigations and metabolic flux analysis (MFA). Enhanced glycolysis, down-regulation of TCA cycle, decoupling of glucose and glutamine utilization, with increased reductive carboxylation of glutamine, so to yield a sustained production of growth building blocks and glutathione, are the hallmarks of enhanced proliferation. Low glucose availability specifically induces cell death in K-ras transformed cells, while PKA activation reverts this effect, possibly through at least two mitochondrial targets. The central role of mitochondria in determining the two investigated cancer cell properties is finally discussed. Taken together the findings reported herein indicate that a system level property is sustained by a cascade of interconnected biochemical pathways that behave differently in normal and in transformed cells