184 research outputs found
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MicroRNA-140 mediates RB tumor suppressor function to control stem cell-like activity through interleukin-6
We established an in vitro cell culture system to determine novel activities of the retinoblastoma (Rb) protein during tumor progression. Rb depletion in p53-null mouse-derived soft tissue sarcoma cells induced a spherogenic phenotype. Cells retrieved from Rb-depleted spheres exhibited slower proliferation and less efficient BrdU incorporation, however, much higher spherogenic activity and aggressive behavior. We discovered six miRNAs, including mmu-miR-18a, -25, -29b, -140, -337, and -1839, whose expression levels correlated tightly with the Rb status and spherogenic activity. Among these, mmu-miR-140 appeared to be positively controlled by Rb and to antagonize the effect of Rb depletion on spherogenesis and tumorigenesis. Furthermore, among genes potentially targeted by mmu-miR-140, Il-6 was upregulated by Rb depletion and downregulated by mmu-mir-140 overexpression. Altogether, we demonstrate the possibility that mmu-mir-140 mediates the Rb function to downregulate Il-6 by targeting its 3\u27-untranslated region. Finally, we detected the same relationship among RB, hsa-miR-140 and IL-6 in a human breast cancer cell line MCF-7. Because IL-6 is a critical modulator of malignant features of cancer cells and the RB pathway is impaired in the majority of cancers, hsa-miR-140 might be a promising therapeutic tool that disrupts linkage between tumor suppressor inactivation and pro-inflammatory cytokine response.Supplementary Table1 and Supplementary Table2: We offer the table data with an Excel fil
Dynamical Studies of Zeolitic Protons and Adsorbates by Picosecond Infrared Spectroscopy(ε ±θ)
A Theory of Electric Field and Charge Density Distributions Inside a Cylindrical Hollow Cathode (ε ±θ)
Formulas for the Analysis of Surface Sum Frequency Generation Spectrum by CH Stuching Modes of Methyl and Methylenoe Groups,
Improved Intensity Formulae for the Determination of Rotational Temperature from the Perturbed Band Structure (ε ±θ)
CO coadsorption-induced recombination of surface hydroxyls to water on Ni(110) surface(ε ±θ)
Detection of Photoinduced Intramolecular Electron Transfer in 4-(1-Pyrenyl)-1- Butyl 9-Anthracenecarboxylate by Transient Absorption and CARS Spectra (ε ±θ)
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