Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johne’s Disease Transmission Dynamics

Mycobacterium avium subspecies paratuberculosis (M. ap), the causative agent of Johne’s disease, infects many farmed ruminants, wild-life animals, and recently isolated from humans. To better understand the molecular pathogenesis of these infections, we analyzed the whole-genome sequences of several M. ap and M. avium subspecies avium (M. avium) isolates to gain insights into genomic diversity associated with variable hosts and environments. Using Next-generation sequencing technology, all six M. ap isolates showed a high percentage of similarity (98%) to the reference genome sequence of M. ap K-10 isolated from cattle. However, two M. avium isolates (DT 78 and Env 77) showed significant sequence diversity (only 87 and 40% similarity, respectively) compared to the reference strain M. avium 104, a reflection of the wide environmental niches of this group of mycobacteria. Within the M. ap isolates, genomic rearrangements (insertions/deletions) were not detected, and only unique single nucleotide polymorphisms (SNPs) were observed among M. ap isolates. While more of the SNPs (~100) in M. ap genomes were non-synonymous, a total of ~6,000 SNPs were detected among M. avium genomes, most of them were synonymous suggesting a differential selective pressure between M. ap and M. avium isolates. In addition, SNPs-based phylo-genomics had a enough discriminatory power to differentiate between isolates from different hosts but yet suggesting a bovine source of infection to other animals examined in this study. Interestingly, the human isolate (M. ap 4B) was closely related to a M. ap isolate from a dairy facility, suggesting a common source of infection. Overall, the identified phylo-genomes further supported the idea of a common ancestor to both M. ap and M. avium isolates. Genome-wide analysis described here could provide a strong foundation for a population genetic structure that could be useful for the analysis of mycobacterial evolution and for the tracking of Johne’s disease transmission among animals

A Novel Approach for Data Encryption Depending on User Location

The wide spread of WLAN and the popularity of mobile devices increases the frequency of data transmission between information system and mobile user. However, most of the data encryption technology is location-independent. An encrypted data can be decrypted anywhere. The encryption technology cannot restrict the location of data decryption. In order to meet the demand of data transmission in the future, a location-dependent approach, called location-dependent data encryption algorithm (LDEA), is proposed in this paper. A target latitude/longitude coordinate is determined firstly. The coordinate is incorporated with a random key for data encryption. The receiver can only decrypt the ciphertext when the coordinate acquired from GPS receiver match with the target coordinate. However, current GPS receiver is inaccuracy and inconsistent. The location of a mobile user is difficult to exactly match with the target coordinate. A toleration distance (TD) is also designed in LDEA to increase its practicality. The security analysis shows that the probability to break LDEA is almost impossible since the length of the random key is adjustable. A prototype is also implemented for experimental study. The results show that the ciphertext can only be decrypted under the restriction of TD. It illustrates that LDEA is effective and practical for data transmission to mobile users

Aluminum alters NMDA receptor 1A and 2A/B expression on neonatal hippocampal neurons in rats

<p>Abstract</p> <p>Background</p> <p>High aluminum (Al) content in certain infant formula raises the concern of possible Al toxicity on brain development of neonates during their vulnerable period of growing. Results of in vivo study showed that Al content of brain tissues reached to 74 μM when oral intake up to 1110 μM, 10 times of that in the hi-Al infant formula.</p> <p>Methods</p> <p>Utilizing a cultured neuron cells in vitro model, we have assessed Al influence on neuronal specific gene expression alteration by immunoblot and immunohistochemistry and neural proliferation rate changes by MTT assay.</p> <p>Results</p> <p>Microscopic images showed that the neurite outgrowth of hippocampal neurons increased along with the Al dosages (37, 74 μM Al (AlCl₃)). MTT results also indicated that Al increased neural cell viability. On the other hand, the immunocytochemistry staining suggested that the protein expressions of NMDAR 1A and NMDAR 2A/B decreased with the Al dosages (p < 0.05).</p> <p>Conclusion</p> <p>Treated hippocampal neurons with 37 and 74 μM of Al for 14 days increased neural cell viability, but hampered NMDAR 1A and NMDAR 2A/B expressions. It was suggested that Al exposure might alter the development of hippocampal neurons in neonatal rats.</p