A Hybrid Meta-heuristic for the Container Loading Problem

It is very common in an enterprise daily operation to solve Container Loading Problem (CLP). Especially, it is an important issue in the logistic management. The problem aims to determine the arrangement of objects with the best utilization ratio in a container. It belongs to the combinatorial optimization problem. In this paper, a two-phased method focusing on the improvement of the efficiency and on the reducing of the problem size is proposed. In the first phase, a constructive method incorporated with a decision rule borrowing from ant colony optimization is used to construct tower set. The pheromone updating mechanism is useful in choosing proper object while constructing tower using decision rule. In the second phase, an improvement method based on genetic algorithm is used. First, the method sorts the towers by the utilization ratio and then assigns a number to each tower accordingly. The chromosome is a sequence of tower numbers which represents the arrangement of towers in the container’s bottom plane. The fitness function is defined as the utilization ratio. A new structure to store the pheromone is proposed which can help the ant in choosing the appropriate object while constructing tower. In this way, the efficiency of the method and the utilization of the container are improved

Albumin fibrillization induces apoptosis via integrin/FAK/Akt pathway

[[abstract]]Background: Numerous proteins can be converted to amyloid-like fibrils to increase cytotoxicity and induce apoptosis, but the methods generally require a high concentration of protein, vigorous shaking, or fibril seed. As well, the detailed mechanism of the cytotoxic effects is not well characterized. In this study, we have developed a novel process to convert native proteins into the fibrillar form. We used globular bovine serum albumin (BSA) as a model protein to verify the properties of the fibrillar protein, investigated its cellular effects and studied the signaling cascade induced by the fibrillar protein. Results: We induced BSA, a non-cytotoxic globular protein, to become fibril by a novel process involving Superdex-200 column chromatography in the presence of anionic or zwittergenic detergent(s). The column pore size was more important than column matrix composite in fibril formation. The fibrillar BSA induced apoptosis in BHK-21 cell as well as breast cancer cell line T47D. Pre-treating cells with anti-integrin antibodies blocked the apoptotic effect. Fibrillar BSA, but not globular BSA, bound to integrin, dephosphorylated focal adhesion kinase (FAK), Akt and glycogen synthase kinase-3β (GSK-3β). Conclusion: We report on a novel process for converting globular proteins into fibrillar form to cause apoptosis by modulating the integrin/FAK/Akt/GSK-3β/caspase-3 signaling pathway. Our findings may be useful for understanding the pathogenesis of amyloid-like fibrils and applicable for the development of better therapeutic agents that target the underlying mechanism(s) of the etiologic agents. ? 2009 Huang et al; licensee BioMed Central Ltd

Diabetes and Health Outcomes Among Older Taiwanese with Hip Fracture

Abstract Objective: Hip fracture tremendously impacts functional abilities for the elderly with high morbidity and mortality; recovery is compromised by co-morbidities. Diabetes mellitus is a common co-morbidity for the aging population, but little is known about the influence of diabetes on outcomes of the Asian elderly with hip fracture. Research Design and Methods: This study was a secondary analysis of data on 242 community-dwelling elders with hip fracture from three previous longitudinal studies. Sixty-one cases (25.2%) had diabetes. Outcomes were measured by the Chinese Barthel Index, Medical Outcomes Study Short Form-36 Taiwan version, and analyzed by the generalized estimating equation approach to examine how diabetes influenced hip-fractured elders' mortality, service utilization, mobility, daily activities, and health-related quality of life during the first 12 months after postsurgical discharge in Taiwan. Results: Hip-fractured elderly with diabetes had a significantly higher mortality rate (22.6% vs. 10.3%, p=0.03) during the first year following discharge, and significantly higher readmission rate (10.0% vs. 2.5%, p=0.04) from the first to third month following discharge than those without diabetes. After controlling for covariates, elderly participants without diabetes had an overall 2.2 times (confidence interval [CI]=1.15?4.21) greater odds of recovery in walking ability and better reported general health (?=9.33; p=0.01) and physical functioning (?=7.26; p=0.02) than those with diabetes during the first year after discharge. Conclusions: Diabetes negatively influenced outcomes of elderly patients with hip fracture. The results may provide a reference for developing interventions for hip-fractured elders with diabetes.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98472/1/rej%2E2011%2E1308.pd