In-vitro Anti-inflammatory and Anti-arthritic Activity of Leaves of Cleodendron Inerme

ABSTRACT Clerodendron inerme belongs to the family Verbenaceae found in southern region of India, which is used in the treatment of Veneral diseases, Rheumatism, Elephantiasis and Intermittent fever. The qualitative phytochemical screening showed the presence of alkaloid, steroids, phenols, flavanoids, tannins, carbohydrates, fixed oils and volatile oils. The Petroleum ether, Chlorofrom, Ethyl acetate, Ethanol and water fractions of the leaves of Clerodendron inerme were subjected to In vitro Anti-inflammatory activity by HRBC membrane stabilization method in various concentration i.e. 10,50,100,200,400,800,1000µg/ml. All the extracts showed positive response as compared to standard Diclofenac sodium. The Ethyl acetate and Ethanol extracts showed maximum activity. The order of effect of different extracts were represented as follows Ethyl acetate> Ethanol >Water> Chloroform> Petroleum ether. The Petroleum ether, Chlorofrom, Ethyl acetate, Ethanol and water fractions of the leaves of Clerodendron inerme were subjected to invitro anti-arthritic activity by protein denaturation method. All the extracts showed positive response .The effect was represented as follows Ethyl acetate> Chloroform>Ethanol> Water> Petroleum ether