Frequency-dependent gating of feedforward inhibition in thalamofrontal synapses

Thalamic recruitment of feedforward inhibition is known to enhance the fidelity of the receptive field by limiting the temporal window during which cortical neurons integrate excitatory inputs. Feedforward inhibition driven by the mediodorsal nucleus of the thalamus (MD) has been previously observed, but its physiological function and regulation remain unknown. Accumulating evidence suggests that elevated neuronal activity in the prefrontal cortex is required for the short-term storage of information. Furthermore, the elevated neuronal activity is supported by the reciprocal connectivity between the MD and the medial prefrontal cortex (mPFC). Therefore, detailed knowledge about the synaptic connections during high-frequency activity is critical for understanding the mechanism of short-term memory. In this study, we examined how feedforward inhibition of thalamofrontal connectivity is modulated by activity frequency. We observed greater short-term synaptic depression during disynaptic inhibition than in thalamic excitatory synapses during high-frequency activities. The strength of feedforward inhibition became weaker as the stimulation continued, which, in turn, enhanced the range of firing jitter in a frequency-dependent manner. We postulated that this phenomenon was primarily due to the increased failure rate of evoking action potentials in parvalbumin-expressing inhibitory neurons. These findings suggest that the MD-mPFC pathway is dynamically regulated by an excitatory-inhibitory balance in an activity-dependent manner. During low-frequency activities, excessive excitations are inhibited, and firing is restricted to a limited temporal range by the strong feedforward inhibition. However, during high-frequency activities, such as during short-term memory, the activity can be transferred in a broader temporal range due to the decreased feedforward inhibition. © 2020 The Author(s).1

Hypoxia with inflammation and reperfusion alters membrane resistance by dynamically regulating voltage-gated potassium channels in hippocampal CA1 neurons

Hypoxia typically accompanies acute inflammatory responses in patients and animal models. However, a limited number of studies have examined the effect of hypoxia in combination with inflammation (Hypo-Inf) on neural function. We previously reported that neuronal excitability in hippocampal CA1 neurons decreased during hypoxia and greatly rebounded upon reoxygenation. We attributed this altered excitability mainly to the dynamic regulation of hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels and input resistance. However, the molecular mechanisms underlying input resistance changes by Hypo-Inf and reperfusion remained unclear. In the present study, we found that a change in the density of the delayed rectifier potassium current (IDR) can explain the input resistance variability. Furthermore, voltage-dependent inactivation of A-type potassium (IA) channels shifted in the depolarizing direction during Hypo-Inf and reverted to normal upon reperfusion without a significant alteration in the maximum current density. Our results indicate that changes in the input resistance, and consequently excitability, caused by Hypo-Inf and reperfusion are at least partially regulated by the availability and voltage dependence of KV channels. Moreover, these results suggest that selective KV channel modulators can be used as potential neuroprotective drugs to minimize hypoxia- and reperfusion-induced neuronal damage. © 2021, The Author(s).1

Real-time data-driven and multi-scale model-guided system for bioproccess digital twin platform

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Effects of Marine Microalgae (Schizochytrium sp.) in Prepared Feeds on Growth and Survival Rate of Juvenile Sea Cucumber Apostichopus japoncus

A 60 day feeding experiment was conducted to evaluate the growth performance and survival rate of the sea cucumber Apostichopus japonicus fed on six experimental diets containing different inclusion level of Schizochytrium algae (0%, 2%, 4%, 6%, 8% and 10%) in a recirculating aquaculture system (RAS). After the feeding trial, survival was not significantly different among the dietary treatments. Results showed that diets affected the specific growth rate (SGR), ingestion rate (IR), faeces production rate (FPR) and food conversion efficiency (FCE) of sea cucumber. SGR of sea cucumber fed diet containing 6% Schizochytrium sp algae was significantly higher than that of sea cucumber fed the other diets (P < 0.05). The lowest IR and FPR was found when sea cucumber fed diets containing 10% Schizochytrium sp. Results of the experiment suggest that dietary inclusion with 6% Schizochytrium sp algae may improve growth of juvenile sea cucumber. Such detailed information could be helpful in further development of more appropriate diets for culture of sea cucumber

Effect of Metal Door On Indoor Radio Channel

This paper reports the variation of indoor radio channel caused by metal door. The simulation results using the Finite Difference Time Domain (FDTD) method and measurement results using the vector network analyzer in frequency domain are used for the characterization of received signal strength variation by metal door. Target frequency bands are three - sensor band, 802.11b ISM band, and 802.11a UNII band. From the simulation and measurement results, the effect of door angle to the received signal strength in three frequency bands and effect of radio frequency to variation are investigated. And, FDTD simulation parameters for different environments are suggested