Assessment of Inter-Specific Diversity of the \u3cem\u3eHedysarum\u3c/em\u3e Genus in Tunisia

In Tunisia, many grassland and pasture species were menaced by genetic erosion. Thus, we were interested in the Hedysarum species which constitute a very important phytogenetic patrimony able to produce forage and restore destroyed pasture land especially in arid and semi-arid areas. In order to facilitate fodder improvement, we investigated the phenetic relationships among Hedysarum species using rDNA intergenic spacer (IGS) polymorphism

Valorisation Agricole D’un Compost Produit À Partir Du Compostage En Cuve Des Déchets Municipaux

This work is in the context of the management and recycling of solid urban waste. The evolution of the physicochemical and microbiological parameters during composting pilot scale household waste was studied. The maturity of the processes and the agronomic value of the finished product were examined. The results showed that the organic fraction of solid waste could be composted successfully in 60 days and revealing a vigorous microbial activity. The final compost has been satisfactory for its agricultural application. Phytotoxicity tests conducted on wheat and tomato crops, show that the incorporation of 25% culture support the compost allows a germination rate of up to 85% against 70% for the control for the variety of corn. In the case of tomatoes, we recorded a germination rate of about 67% against 58% for the control

Total sleep deprivation alters endothelial function in rats: a nonsympathetic mechanism

STUDY OBJECTIVES: Sleep loss is suspected to induce endothelial dysfunction, a key factor in cardiovascular risk. We examined whether sympathetic activity is involved in the endothelial dysfunction caused by total sleep deprivation (TSD). DESIGN: TWO GROUPS: TSD (24-h wakefulness), using slowly rotating wheels, and wheel control (WC). PARTICIPANTS: Seven-month-old male Wistar rats. INTERVENTIONS: Pharmacological sympathectomy (reserpine, 5 mg/kg, intraperitoneal), nitric oxide synthase (NOS) inhibition (N (G)-nitro-L-arginine, 20 mg/kg, intraperitoneally 30 min before experiment) and cyclooxygenase (COX) inhibition (indomethacin, 5 mg/kg, intraperitoneally 30 min before experiment). MEASUREMENTS AND RESULTS: In protocol 1, changes in heart rate (HR) and blood pressure were continuously recorded in the sympathectomized and non-sympathectomized rats. Blood pressure and HR increased during TSD in non-sympathectomized rats. In protocol 2, changes in skin blood flow (vasodilation) were assessed in the sympathectomized and non-sympathectomized rats using laser-Doppler flowmetry coupled with iontophoretic delivery of acetylcholine (ACh), sodium nitroprusside (SNP), and anodal and cathodal currents. ACh- and cathodal current-induced vasodilations were significantly attenuated after TSD in non-sympathectomized and sympathectomized rats (51% and 60%, respectively). In protocol 3, ACh-induced vasodilation was attenuated after NOS and COX inhibition (66% and 49%, respectively). Cathodal current-induced vasodilation decreased by 40% after COX inhibition. In TSD compared to WC a decrease in ACh-induced vasodilation was still observed after COX inhibition. No changes in SNP- and anodal current-induced vasodilation were detected. CONCLUSION: These results demonstrate that total sleep deprivation induces a reduction in endothelial-dependent vasodilation. This endothelial dysfunction is independent of blood pressure and sympathetic activity but associated with nitric oxide synthase and cyclooxygenase pathway alterations

LANDSLIDE SUSCEPTIBILITY MAPPING IN THE MUNICIPALITY OF OUDKA, NORTHERN MOROCCO: A COMPARISON BETWEEN LOGISTIC REGRESSION AND ARTIFICIAL NEURAL NETWORKS MODELS

The Rif is among the areas of Morocco most susceptible to landslides, because of the existence of relatively young reliefs marked by a very important dynamics compared to other regions. These landslides are one of the most serious problems on many levels: social, economic and environmental. The increase in the frequency and impact of landslides over the past decade has demonstrated the need for an in-depth study of these phenomena, allowing the identification of areas susceptible to landslides. The main objective of this study is to identify the optimal method for the mapping of the area susceptible to landslides in municipality of Oudka. This area has been marked by the largest landslide in the region, caused by heavy rainfall in 2013. Two Statistical Methods i) Regression Logistics (LR) ii) Artificial Neural Networks (ANN), were used to create a landslide susceptibility map. The realization of this susceptibility map required, first, the mapping of old landslides by the aerial photography, the data of the geological map and by the data obtained using field surveys using GPS. A total of 105 landslides were mapped from these various sources. 50% of this database was used for model building and 50% for validation. Eight independent landslide factors are exploited to detect the most sensitive areas: altitude, slope, aspect, distance of faults, distance streams, distance from roads, lithology and vegetation index (NDVI). The results of the landslide susceptibility analysis were verified using success and prediction rates. The success rate (AUC&thinsp;=&thinsp;0.918) and the prediction rate (AUC&thinsp;=&thinsp;0.901) of the LR model is higher than that of the ANN model (success rate (AUC&thinsp;=&thinsp;0.886) and prediction rate (AUC&thinsp;=&thinsp;0.877). These results indicate that the Regression Logistic (LR) model is the best model for determining landslide susceptibility in the study area.</p

Sub-Banded Reconstructed Phase Spaces for Speech Recognition

A novel method combining filter banks and reconstructed phase spaces is proposed for the modeling and classification of speech. Reconstructed phase spaces, which are based on dynamical systems theory, have advantages over spectral-based analysis methods in that they can capture nonlinear or higher-order statistics. Recent work has shown that the natural measure of a reconstructed phase space can be used for modeling and classification of phonemes. In this work, sub-banding of speech, which has been examined for recognition of noise-corrupted speech, is studied in combination with phase space reconstruction. This sub-banding, which is motivated by empirical psychoacoustical studies, is shown to dramatically improve the phoneme classification accuracy of reconstructed phase space-based approaches. Experiments that examine the performance of fused sub-banded reconstructed phase spaces for phoneme classification are presented. Comparisons against a cepstral-based classifier show that the proposed approach is competitive with state-of-the-art methods for modeling and classification of phonemes. Combination of cepstral-based features and the sub-band RPS features shows improvement over a cepstral-only baseline

Effect of acute sleep deprivation on vascular function in healthy subjects

Sleep disorders are associated with inflammation and sympathetic activation, which are suspected to induce endothelial dysfunction, a key factor in the increased risk of cardiovascular disease. Less is known about the early effects of acute sleep deprivation on vascular function. We evaluated microvascular reactivity and biological markers of endothelial activation during continuous 40 h of total sleep deprivation (TSD) in 12 healthy men (29 +/- 3 yr). The days before [day 1 (D1)] and during TSD (D3), at 1200 and 1800, endothelium-dependent and -independent cutaneous vascular conductance was assessed by iontophoresis of acetylcholine and sodium nitroprusside, respectively, coupled to laser-Doppler flowmetry. At 0900, 1200, 1500, and 1800, heart rate (HR) and instantaneous blood pressure (BP) were recorded in the supine position. At D1, D3, and the day after one night of sleep recovery (D4), markers of vascular endothelial cell activation, including soluble intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin, and interleukin-6 were measured from blood samples at 0800. Compared with D1, plasma levels of E-selectin were raised at D3, whereas intercellular adhesion molecule-1 and interleukin-6 were raised at D4 (P &lt; 0.05). The endothelium-dependent and -independent CVC were significantly decreased after 29 h of TSD (P &lt; 0.05). By contrast, HR, systolic BP, and the normalized low-frequency component of HR variability (0.04-0.15 Hz), a marker of the sympathetic activity, increased significantly within 32 h of TSD (P &lt; 0.05). In conclusion, acute exposure to 40 h of TSD appears to cause vascular dysfunction before the increase in sympathetic activity and systolic BP

Variable number of tandem repeat polymorphisms of the interleukin-1 receptor antagonist gene IL-1RN: a novel association with the athlete status

<p>Abstract</p> <p>Background</p> <p>The interleukin-1 (IL-1) family of cytokines is involved in the inflammatory and repair reactions of skeletal muscle during and after exercise. Specifically, plasma levels of the IL-1 receptor antagonist (IL-1ra) increase dramatically after intense exercise, and accumulating evidence points to an effect of genetic polymorphisms on athletic phenotypes. Therefore, the IL-1 family cytokine genes are plausible candidate genes for athleticism. We explored whether IL-1 polymorphisms are associated with athlete status in European subjects.</p> <p>Methods</p> <p>Genomic DNA was obtained from 205 (53 professional and 152 competitive non-professional) Italian athletes and 458 non-athlete controls. Two diallelic polymorphisms in the IL-1β gene (<it>IL-1B</it>) at -511 and +3954 positions, and a variable number tandem repeats (VNTR) in intron 2 of the IL-1ra gene (<it>IL-1RN</it>) were assessed.</p> <p>Results</p> <p>We found a 2-fold higher frequency of the <it>IL-1RN </it>1/2 genotype in athletes compared to non-athlete controls (OR = 1.93, 95% CI = 1.37-2.74, 41.0% vs. 26.4%), and a lower frequency of the 1/1 genotype (OR = 0.55, 95% CI = 0.40-0.77, 43.9% vs. 58.5%). Frequency of the <it>IL-1RN </it>2/2 genotype did not differ between groups. No significant differences between athletes and controls were found for either -511 or +3954 <it>IL-1B </it>polymorphisms. However, the haplotype (-511)C-(+3954)T-(VNTR)2 was 3-fold more frequent in athletes than in non-athletes (OR = 3.02, 95% CI = 1.16-7.87). Interestingly, the <it>IL-1RN </it>1/2 genotype was more frequent in professional than in non-professional athletes (OR = 1.92, 95% CI = 1.02-3.61, 52.8% vs. 36.8%).</p> <p>Conclusions</p> <p>Our study found that variants at the IL-1ra gene associate with athletic status. This confirms the crucial role that cytokine IL-1ra plays in human physical exercise. The VNTR <it>IL-1RN </it>polymorphism may have implications for muscle health, performance, and/or recovery capacities. Further studies are needed to assess these specific issues. As VNTR <it>IL-1RN </it>polymorphism is implicated in several disease conditions, athlete status may constitute a confounding variable that will need to be accounted for when examining associations of this polymorphism with disease risk.</p

Hypothalamic inflammation is reversed by endurance training in anorectic-cachectic rats

<p>Abstract</p> <p>Aim</p> <p>We tested the effects of a cancer cachexia-anorexia sydrome upon the balance of anti and pro-inflammatory cytokines in the hypothalamus of sedentary or trained tumour-bearing (Walker-256 carcinosarcoma) rats.</p> <p>Methods</p> <p>Animals were randomly assigned to a sedentary control (SC), sedentary tumour-bearing (ST), and sedentary pair-fed (SPF) groups or, exercised control (EC), exercised tumour-bearing (ET) and exercised pair-fed (EPF) groups. Trained rats ran on a treadmill (60%VO_2max) for 60 min/d, 5 days/wk, for 8 wks. We evaluated food intake, leptin and cytokine (TNF-α, IL1β) levels in the hypothalamus.</p> <p>Results</p> <p>The cumulative food intake and serum leptin concentration were reduced in ST compared to SC. Leptin gene expression in the retroperitoneal adipose tissue (RPAT) was increased in SPF in comparison with SC and ST, and in the mesenteric adipose tissue (MEAT) the same parameter was decreased in ST in relation to SC. Leptin levels in RPAT and MEAT were decreased in ST, when compared with SC. Exercise training was also able to reduce tumour weight when compared to ST group. In the hypothalamus, IL-1β and IL-10 gene expression was higher in ST than in SC and SPF. Cytokine concentration in hypothalamus was higher in ST (TNF-α and IL-1β, p < 0.05), compared with SC and SPF. These pro-inflammatory cytokines concentrations were restored to control values (p < 0.05), when the animals were submitted to endurance training.</p> <p>Conclusion</p> <p>Cancer-induced anorexia leads towards a pro-inflammatory state in the hypothalamus, which is prevented by endurance training which induces an anti-inflammatory state, with concomitant decrease of tumour weight.</p