Liposclerosing myxofibrous tumor of the distal femur: A case report

IntroductionLiposclerosing myxofibrous tumor (LSMFT) is a rare benign fibro-osseous tumor that most frequently occurs in the proximal femur. The reported literature shows that the proximal femur, ilium, tibia, humerus, rib, and skull have occurred, but so far, the female distal femur has not been characterized in detail. This, we think, is the first single comprehensive case report of the female distal femur. To prevent misdiagnosis and overtreatment of this illness, it is critical for us to continue strengthening our knowledge of it and to add it to the differential diagnosis of the space-occupying lesion of the female distal femur.Case summaryTwo months ago, a 55-year-old female patient was found to have a space-occupying lesion of the left distal femur and the pain symptom was aggravated. She underwent thorough curettage and bone grafting without additional treatment to relieve the current symptoms and determine the nature of the lesion in our hospital. The intraoperative specimens were submitted to the pathology laboratory for analysis, and the result was reported as LSMFT. And six months after the operation, the patient returned to our hospital for another x-ray examination and we found that she had recovered well without any signs of recurrence. The patient self-reported that she had now resumed her daily life without any uncomfortable symptoms.ConclusionThe incidence of LSMFT itself is relatively low, and the occurrence of the distal femur is even rarer. However, it is recommended to add LSMFT into the differential diagnosis of the occupying lesions of the distal femur. Once the diagnosis is made, thorough curettage and bone grafting without additional special treatment can achieve better postoperative outcomes. The patient gave her agreement after learning that information about the case will be submitted for publication

Gene markers of fracture healing in early stage and the regulatory mechanism during the process using microarray analysis

Background: The aim of this study was to explore crucial markers and uncover the regulatory mechanisms of fracture healing in the early stage. Methods: Gene expression profile of GSE45156 was downloaded, in which 3 fractured samples and 3 unfractured samples were used in our present study. Based on the threshold value, differentially expressed genes (DEGs) were selected between two kinds of samples using limma package in R. Enrichment analysis of these DEGs was performed by DAVID software. Furthermore, protein-protein interaction (PPI) network was established integrating information in STRING database, and visualized by Cytoscape software. Results: We identified a set of 960 DEGs including 509 up-regulated and 451 downregulated genes. Biological processes involving RNA splicing and cell cycle were significantly enriched for the up-regulated genes such as Snrpd2, Eftud2, Plk1 and Bub1b, whereas skeletal system development and bone development processes were predominant for down-regulated genes like Ubc. In the constructed PPI network, all the five genes were the predominant nodes, of which Snrpd2 was linked to Eftud2, while Bub1b was to interact with Plk1. Conclusion: Five candidate genes crucial for indicating the process of fracture in early stage were identified. Eftud2, Snrpd2, Bub1b and Plk1 might function through the involvement of cell-cycle-related BP, while Ubc might influence the protein degradation during bone development. However, more experimental validations are needed to confirm these results. Keywords: Fracture healing, Early stage, RNA splicing, Cell cycle, Protein degradation, Protein–protein interactio

In vivo inhibitory effect of suberoylanilide hydroxamic acid combined with sorafenib on human hepatocellular carcinoma cells

The present study aimed to investigate the in vivo inhibitory effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) combined with sorafenib on human hepatocellular carcinoma HCCLM3 cells. The nude mice transplanted with HCCLM3 cells were randomly divided into control, SAHA, sorafenib and SAHA+sorafenib groups. The nude mice in the later 3 groups were intragastrically administrated with SAHA (10 mg·kg-1·day-1), sorafenib (10 mg·kg-1·day-1) and SAHA (10 mg·kg-1·day-1) combined with sorafenib (10 mg·kg-1·day-1), respectively, for successive 20 days. Finally, the inhibition rate of tumor was measured. The expressions of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, Bax, p53, MMP2, MMP-9 and uPA in tumor tissues were determined. Results showed that, compared with SAHA and Sorafenib groups, in SAHA+sorafenib groups the inhibition rate of tumor was significantly increased (P < 0.05), the expression levels of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, MMP-2 and MMP-9 and uPA protein in tumor tissues were significantly decreased, respectively (P < 0.05), and the expression levels of Bax and p53 protein were significantly increased, respectively (P < 0.05). In conclusion, compared with single drug, SAHA combined with sorafenib can enhance the inhibitory effects on HCCLM3 xenografts in nude mice

Using an integrated feature-based molecular network and lipidomics approach to reveal the differential lipids in yak shanks and flanks

Yak shanks and flanks are often used as food ingredients, but the lipid composition of these two parts may differ significantly. These meat parts were subjected to a lipidomics analysis using UHPLC-Q-Obitrap. Several computational tools, including feature-based molecular networks, ms-dial, and lipidone, were used to perform deep mining on the entire dataset. The analysis annotated 355 lipid species from 20 subclasses. Lipid chains have a length distribution of 16 to 20 carbons, with unsaturation ranging from 0 to 5. The results revealed that 71 lipids were significantly different in these muscles, including phosphatidylethanolamines (PEs) (16:0/20:4), PEs (18:0/19:1), PEs (18:1/22:5), sphingomyelins (SMs) (36:2; 3O), and carnitines (CARs) (22:0). Furthermore, the metabolic pathways of glycerophospholipids and sphingolipids act as important roles in the differences of these lipid components. This study obtained a comprehensive lipid profile, which is critical for understanding the precise nutritional differences in different yak meat sections

Ratio of metastatic to examined lymph nodes, a helpful staging system and independent prognostic factor of esophagogastric junction cancer.

BACKGROUND: The incidence of the esophagogastric junction cancer is growing rapidly. The purpose of this study is to clarify the outcome of the ratio between metastatic and examined lymph nodes in esophagogastric junction cancer patients with or without 7 examined lymph nodes. METHODS: A total of 3,481 patients who underwent operation are identified from the Surveillance, Epidemiology, and End Results database. Different lymph nodes resected groups are analyzed to test the lymph nodes ratio factor. RESULTS: There are 2522 patients with 7 or more lymph nodes resected and 959 patients with less than 7 lymph nodes resected. Lymph nodes ratio and lymph node involvement are independent prognostic factors. But the lymph nodes ratio categories have a better prognostic value than the lymph node involvement categories. Compared with lymph node involvement categories, lymph nodes ratio categories represent patients with more homogeneous overall survival rate. CONCLUSIONS: This study defines that the lymph nodes ratio is an independent prognostic factor for esophagogastric junction cancer. The lymph nodes ratio can prevent stage migration and may be a helpful system to predict the prognosis of esophagogastric junction cancer patients

Predicting the Release and Migration of Potentially Harmful Elements (PHEs) during the Lightweight Ceramsite Preparation from Carbide Slag

When preparing lightweight ceramsite using carbide slag, trace amounts of toxic elements are released into the atmosphere due to high-temperature calcination, posing a significant risk to the environment. The real-time monitoring of the released gases is challenging under laboratory conditions while preparing large quantities of ceramsite. Therefore, heating was simulated using experimental data and the FactSage 7.0 thermochemical database to study the release of harmful Al-, C-, H-, S-, and F-containing elements when using carbide slag to prepare lightweight ceramsite. The results indicated that no Al, C, H, S, or F elements were evident in the high-temperature liquid products obtained in a 50 °C to 1150 °C calcination temperature range. Al was present in a solid state with no gaseous products. When the temperature reached 450 °C, CO gas was released and its level increased as the temperature rose. H and S mainly combined into H2S gas, starting at 250 °C and reaching a peak at 1050 °C. H and F primarily combined into HF, starting at 400 °C. Other F-containing gases mainly included SiF4 and TiF3, which began to release at 800 °C and 900 °C, respectively. The release trends of HF, SiF4, and TiF3 were consistent with those of CO. This study aimed to conduct an environmental impact and management assessment for the preparation of lightweight ceramsite using carbide slag. The use of raw material carbide slag for the low-cost treatment of tail gas was proposed, which provides theoretical and up-to-date support for greening the application of the process

Short-Chain Fatty Acids Weaken Ox-LDL-Induced Cell Inflammatory Injury by Inhibiting the NLRP3/Caspase-1 Pathway and Affecting Cellular Metabolism in THP-1 Cells

Short-chain fatty acids (SCFAs) are important anti-inflammatory metabolites of intestinal flora. Oxidized low-density lipoprotein (ox-LDL)-induced macrophage activation is critical for the formation of atherosclerosis plaque. However, the association between SCFAs and ox-LDL-induced macrophage activation with respect to the formation of atherosclerosis plaque has not yet been elucidated. The present study investigated whether SCFAs (sodium acetate, sodium propionate, and sodium butyrate) can affect ox-LDL-induced macrophage activation and potential signaling pathways via regulation of the expression of the NLRP3/Caspase-1 pathway. Using human monocyte-macrophage (THP-1) cells as a model system, it was observed that ox-LDL not only induced cell inflammatory injury but also activated the NLRP3/Caspase-1 pathway. The exogenous supplementation of three SCFAs could significantly inhibit cell inflammatory injury induced by ox-LDL. Moreover, three SCFAs decreased the expression of IL-1β and TNF-α via the inactivation of the NLRP3/Caspase-1 pathway induced by ox-LDL. Furthermore, three SCFAs affected cellular metabolism in ox-LDL-induced macrophages, as detected by untargeted metabolomics analysis. The results of the present study indicated that three SCFAs inhibited ox-LDL-induced cell inflammatory injury by blocking the NLRP3/Caspase-1 pathway, thereby improving cellular metabolism. These findings may provide novel insights into the role of SCFA intervention in the progression of atherosclerotic plaque formation

Development of the Peritoneal Metastasis: A Review of Back-Grounds, Mechanisms, Treatments and Prospects

Peritoneal metastasis is a malignant disease which originated from several gastrointestinal and gynecological carcinomas and has been leading to a suffering condition in patients for decades. Currently, as people have gradually become more aware of the severity of peritoneal carcinomatosis, new molecular mechanisms for targeting and new treatments have been proposed. However, due to the uncertainty of influencing factors involved and a lack of a standardized procedure for this treatment, as well as a need for more clinical data for specific evaluation, more research is needed, both for preventing and treating. We aim to summarize backgrounds, mechanisms and treatments in this area and conclude limitations or new aspects for treatments

Predicting the Release and Migration of Potentially Harmful Elements (PHEs) during the Lightweight Ceramsite Preparation from Carbide Slag

When preparing lightweight ceramsite using carbide slag, trace amounts of toxic elements are released into the atmosphere due to high-temperature calcination, posing a significant risk to the environment. The real-time monitoring of the released gases is challenging under laboratory conditions while preparing large quantities of ceramsite. Therefore, heating was simulated using experimental data and the FactSage 7.0 thermochemical database to study the release of harmful Al-, C-, H-, S-, and F-containing elements when using carbide slag to prepare lightweight ceramsite. The results indicated that no Al, C, H, S, or F elements were evident in the high-temperature liquid products obtained in a 50 °C to 1150 °C calcination temperature range. Al was present in a solid state with no gaseous products. When the temperature reached 450 °C, CO gas was released and its level increased as the temperature rose. H and S mainly combined into H2S gas, starting at 250 °C and reaching a peak at 1050 °C. H and F primarily combined into HF, starting at 400 °C. Other F-containing gases mainly included SiF4 and TiF3, which began to release at 800 °C and 900 °C, respectively. The release trends of HF, SiF4, and TiF3 were consistent with those of CO. This study aimed to conduct an environmental impact and management assessment for the preparation of lightweight ceramsite using carbide slag. The use of raw material carbide slag for the low-cost treatment of tail gas was proposed, which provides theoretical and up-to-date support for greening the application of the process