Intraspecific variations in leaf functional traits of Cunninghamia lanceolata provenances

BackgroundStudies on intra-specific variability in leaf functional traits is important to evaluate adaptation of the species to predicted climate change, and to develop long-term conservation strategy. The main objectives were to investigate the relationship between the functional traits leaves and C, N, P stoichiometry of Chinese fir from different geographical provenances and their relationship with the main environmental factors of provenance.ResultsIn this study, we measured 12 leaf functional traits on 36-year-old Cunninghamia lanceolata trees from 13 provenances. Analysis of variance (ANOVA) was performed to examine the variability. Redundancy analysis (RA) was computed to examine the relationship between geo-climatic factors of provenance origin and leaf functional traits while Pearson's correlation coefficient was computed to assess inter-trait correlations. The results showed statistically significant differences (P < 0.01) in intraspecific leaf traits among provenances, except leaf P content. The relationships among leaf traits are consistent with the general trend observed in the leaf economic spectrum. Mean annual temperature appeared to be a key factor that influences intraspecific leaf traits variability compared to mean annual precipitation.ConclusionThese results provide useful insights about adaptation of leaf trait of Chinese fir in a changing climatic condition. Thus, our findings shed light on the importance of interspecific trait variability in Chinese fir and the potential effect of climate change

Growth Rate and Leaf Functional Traits of Four Broad-Leaved Species Underplanted in Chinese Fir Plantations with Different Tree Density Levels

The close-to-Nature management method of interplanting broad-leaved trees after thinning of monoculture plantations is an important mixed species restoration model to improve the ecological service and functions effectively as well as to reduce the productivity decline of the multi-generation continuous planting of monoculture. Thus, the selection of tree species for establishing mixed forest and its ecological adaptability are the key issues. In this study, we conducted thinning experiment in an 11-year-old Chinese fir plantation with retention density of 900 trees/ha, 1200 trees/ha and 1875 trees/ha, and then underplanted four broad-leaved species, Schima superba, Phoebe bournei, Tsoongiodendron odorum and Michelia macclurei. After three years, we analyzed the growth rate and leaf functional traits of the broad-leaved species and their correlations with stand characteristics. The results showed that growth rate of seedlings of the four broad-leaved species were significantly different (p < 0.05) among different tree density levels and species. Low tree density favored seedling growth compared with high tree density and seedlings of T. odorum and S. superba performed best. However, leaf functional traits varied significantly (p < 0.01) among species only, and T. odorum had the largest specific leaf area, the smallest leaf mass per unit area, the smallest leaf tissue density, relatively large leaf thickness, and relatively small dry matter content. The leaf C content varied significantly among tree density levels and species; leaf N content varied significantly among species only; and leaf p content varied among tree density levels only. Correlation analyses between growth characters and leaf functional traits showed that height growth was significantly correlated with leaf N content (r = 0.686; p = 0.041) and with C:N ratio (r = -0.682; p = 0.043). Root collar diameter growth was significantly correlated with specific leaf area (r = 0.820; p = 0.007), leaf N content (r = 0.685; p = 0.042), leaf thickness (r = -0.706; p = 0.034) and leaf mass per unit area (r = -0.812; p = 0.008). Thus, leaf functional traits possibly predict diameter growth better than height growth. As a whole, growth rate and leaf functional traits could be used as a guide for selection of species for under planting in thinned pure monoculture plantations to establish conifer-broadleaved mixed forests. Based on growth rate and leaf functional traits, T. odorum appeared to be suitable for planting under low tree density stands where the degree of shading is low