Research on multifunctional integrated intelligent stair-climbing chair based on ergonomics

In view of the practical problems faced by the group with travel inconvenience, the wheelchairs in today’s Chinese market can’t meet the needs of society. Taking the integrated function chair as the research object, this paper puts forward the whole structure of the self-help ladder climbing intelligent chair. The chair is designed on the basis of analyzing the working principle of the main and the auxiliary structure. Based on Ergonomics, the composition and driving mode of each core module and auxiliary mechanism are developed, and the three-dimensional model of self-help ladder climbing intelligent chair is established by using UG. Selfhelp intelligent stair-climbing chair is not only conductive to improving the quality of life of special people and easing their family burden, but also has positive significance to the healthy and sustainable development of wheelchair industry

Multimodal optimization using whale optimization algorithm enhanced with local search and niching technique

For some real-world problems, it is desirable to find multiple global optima as many as possible. The multimodal optimization approach which finds multiple optima in a single run shows significant difference with the single modal optimization approach.The whale optimization algorithm (WOA) is a newly emerging reputable optimization algorithm. Its global search ability has been verified in many benchmark functions and real-world applications. In this paper, we propose a multimodal version of whale optimization algorithm (MMWOA). MMWOA enhances the multimodal search ability of WOA by using the niching technique and improves the local search efficiency of WOA by combining the Gaussian sampling technique. The algorithm has been tested on multimodal optimization benchmark functions recommended by CEC'2013 and on a multimodal optimization problem with non-linear constraints. Experimental results indicate that MMWOA has competitive performance compared with other state-of-the-art multimodal optimization algorithms

Fatty Acid Desaturation Is Suppressed in Mir-26a/b Knockout Goat Mammary Epithelial Cells by Upregulating <i>INSIG1</i>

MicroRNA-26 (miR-26a and miR-26b) plays a critical role in lipid metabolism, but its endogenous regulatory mechanism in fatty acid metabolism is not clear in goat mammary epithelial cells (GMECs). GMECs with the simultaneous knockout of miR-26a and miR-26b were obtained using the CRISPR/Cas9 system with four sgRNAs. In knockout GMECs, the contents of triglyceride, cholesterol, lipid droplets, and unsaturated fatty acid (UFA) were significantly reduced, and the expression of genes related to fatty acid metabolism was decreased, but the expression level of miR-26 target insulin-induced gene 1 (INSIG1) was significantly increased. Interestingly, the content of UFA in miR-26a and miR-26b simultaneous knockout GMECs was significantly lower than that in wild-type GMECs and miR-26a- and miR-26b-alone knockout cells. After decreasing INSIG1 expression in knockout cells, the contents of triglycerides, cholesterol, lipid droplets, and UFAs were restored, respectively. Our studies demonstrate that the knockout of miR-26a/b suppressed fatty acid desaturation by upregulating the target INSIG1. This provides reference methods and data for studying the functions of miRNA families and using miRNAs to regulate mammary fatty acid synthesis

Ideal Cardiovascular Health Metrics Are Associated with Reduced Severity of Hepatic Steatosis and Liver Fibrosis Detected by Transient Elastography

Life&rsquo;s Simple 7 (LS7) is the American Heart Association&rsquo;s (AHA) proposal for a healthy lifestyle, also known as cardiovascular health (CVH) metrics. However, the association between CVH metrics and the severity of hepatic steatosis and liver fibrosis detected by transient elastography is unknown. We performed a cross-sectional study using the data from the 2017&ndash;2018 National Health and Nutrition Examination Survey (NHANES) cycle. The controlled attenuation parameter (CAP) and liver stiffness measurement (LSM) were used to evaluate the severity of hepatic steatosis and liver fibrosis and to define NAFLD, advanced liver fibrosis, and cirrhosis. A total of 2679 participants were included. Multivariate linear regression analysis revealed that per 1-unit increase in the CVH metric, CAP and LSM decreased by 8.565 units and 0.274 units, respectively. In the multivariate logistic regression analysis, the risk of NAFLD, advanced liver fibrosis, and cirrhosis were 7, 10, and 6 times higher in the poor CVH group than in the ideal CVH group. Subgroup analysis indicated that CVD patients and non-Hispanic whites could benefit more from ideal CVH. In conclusion, adherence to ideal CVH metrics, as proposed by the AHA, can significantly reduce the risk of hepatic steatosis and liver fibrosis

Elucidating the formation and structural evolution of platinum single-site catalysts for hydrogen evolution reaction

Platinum single-site catalysts (SSCs) are a promising technology for the production of hydrogen from clean energy sources. They have high activity and maximal platinum-atom utilisation. However, the bonding environment of platinum during operation is poorly understood. In this work, we use operando, synchrotron-X-ray absorption spectroscopy to study the platinum bonding in SSCs. First, we synthesise an atomically dispersed platinum complex with aniline and chloride ligands onto graphene and characterise it with ex-situ electron microscopy, X-ray diffractometry, X-ray photoelectron spectroscopy, X-ray absorption near edge structure spectroscopy (XANES), and extended X-ray absorption fine structure spectroscopy (EXAFS). Then, by operando EXAFS and XANES, we show that as a negatively biased potential is applied, the Pt-N bonds break first followed by the Pt-Cl bonds. The platinum is reduced from platinum(II) to metallic platinum(0) by the onset of the hydrogen-evolution reaction at 0 V. Furthermore, we observe an increase in Pt-Pt bonding, indicating the formation of platinum agglomerates. Together, these results indicate that while aniline is used to prepare platinum SSCs, the single-site complexes are decomposed and platinum agglomerates at operating potentials. This work is an important contribution to the understanding of the bonding environment and the evolution of the molecular structure of platinum complexes in SSCs

Breeding of the Long-Grain Restorer of <i>Indica</i>-<i>Japonica</i> Hybrid Rice by Using the Genetic Effects of Grain Shape QTLs

Grain shape improvement, which determines grain yield, quality traits and commercial value, is an extremely important aspect of rice breeding. Grain size is controlled by multiple genes, and Maker Assistant Selection (MAS) breeding is effective for breeders in developing stable and efficient markers to aggregate these genes in order to speed up the selection of new lines with desirable traits during the breeding process. In this study, functional markers were developed based on the sequence differences of five grain-shaped genes (GL7, GW6a, GS6, GW5 and TGW6) between the long-grain japonica rice variety Zhendao and the indica-japonica restorer R2027. We then constructed a population of recombinant inbred lines (RILs) based on their cross. The newly designed functional markers were used to genotype grain-size genes, and a genetic effect analysis was conducted to screen high-quality long-grain restorers. Our results reveal diverse effects of different genes on grain size, and the five genotypes were distributed in the 36 selected BC1F8 lines. Specifically, gw5 positively regulates grain width and 1000-grain weight, gl7 and gs6 positively regulate grain length but negatively regulate grain width and 1000-grain weight, tgw6 positively regulates grain length and gw6a positively regulates 1000-grain weight. The most outstanding outcome is that 5 of the 36 lines achieved in this study showing an excellent performance of long grain and yield characters are ideal materials not only for studying the interaction and genetic effects between polygenes but also as restorers or donors for dominant genes in indica-japonica hybrid rice breeding