MiR-10b alleviates high glucose-induced human retinal endothelial cell injury by regulating TIAM1 signaling

Purpose: To investigate the effects of microRNA (miR)-10b on high glucose (HG)-induced human retinal endothelial cell (HREC) injury and the mechanisms involved.Methods: Levels of miR-10b were measured in HRECs using quantitative reverse transcriptasepolymerase chain reaction (qRT-PCR) after the addition of glucose (5.5 and 30 mM). Cell viability was measured using Cell Counting Kit-8 assay, while levels of reactive oxygen species (ROS) weredetermined using fluorimetry. An enzyme-linked immunosorbent assay (ELISA) was used to measure cellular apoptosis. Luciferase reporter assay was used to validate the miR-10b-binding sites of target genes. The levels of T-cell lymphoma invasion and metastasis (TIAM1) and NADPH oxidase-2 (NOX2) were determined using qRT-PCR. Ras-related C3 botulinum toxin substrate 1 (Rac1) activation was evaluated using a pull-down assay. The protein levels of TIAM1 and Rac1 were assayed by western blotting.Results: After HG stimulation, miR-10b expression was downregulated. Viability of HRECs decreased, whereas ROS production increased. However, the overexpression of miR-10b inhibited apoptosis and ROS production in HG-treated HRECs (p < 0.05), while luciferase reporter analysis revealed a possible binding site for miR-10b to target the 3'-untranslated region (UTR) of TIAM1. In addition, the overexpression of miR-10b distinctly reduced the expression levels of TIAM1 and NOX2, but decreased the activation of Rac1 in HG-treated HRECs (p < 0.05); these inhibitory effects of miR-10b were significantly reversed after TIAM1 application.Conclusion: MiR-10b alleviates HG-induced HREC injury by regulating TIAM1 signaling. MiR-10b therapy is a potential therapeutic strategy for patients suffering from diabetic retinopathy. Keywords: MicroRNA-10b, Human retinal endothelial cells, High glucose, TIAM1-Rac1 axi

Retracted: MiR-10b alleviates high glucose-induced human retinal endothelial cell injury by regulating TIAM1 signaling

This article previously published in Volume 19 Issue 8 of this journal in August 2020 has been retracted in line with the guidelines from the Committee on Publication Ethics (COPE, http://publication ethics.org/resources/guidelines).Retraction: Chen Y, Zhu Y, Zhao S. MiR-10b alleviates high glucose-induced human retinal endothelial cell injury by regulating TIAM1 signaling. Trop J Pharm Res, 2020, 19(8): 1577-1583.To the editor:I am retracting this article because some of the results we presented are irreproducible.Signed: Sheng Zha

Study of Deformation-Compensated Modeling for Flexible Material Path Processing Based on Fuzzy Neural Network and Fuzzy Clustering

In this paper, the Flexible Material Path Processing (FMPP) deformation compensation modeling method based on T-S fuzzy neural network is proposed. This method combined with T S fuzzy reasoning and fuzzy neural network.Firstly, fuzzy clustering is introduced to extract fuzzy membership functions and the fitness of fuzzy rules of T S fuzzy neural network antecedent from historical processing data; secondly, through back-propagation iteration to calculate connection weights of the network. Processing experiments shows that T S fuzzy neural network modeling in this paper is superior to typical T S model，the angle error and straightness error processing by NTS FNN is decreased than these of STS FNN

Dynamic Modelling of an Automated Vehicle Storage and Retrieval System and a Simulation Analysis of its Efficiency

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Hemarthria compressa is an important warm-season forage grass for use in Southwest China. However, due to poor seed set, it is propagated by vegetative cuttings of stolons, rhizomes, and nodal sections. The in vitro propagation of H. compressa is still faced with difficulties including blackening or browning of tissues prior to culturing due to the oxidation of phenolic compounds by polyphenolic oxidase enzyme present in excised tissue (Yang et al. 2008). The objectives of the study were to investigate possible means of successful initiation of cultures through elimination of phenolic browning

Deformation-compensated modeling of flexible material processing based on T-S fuzzy neural network and fuzzy clustering

According to the factors that influence flexible material processing (FMP), the deformation compensation modeling method based on T-S fuzzy neural network is proposed. This method combines T-S fuzzy reasoning with a fuzzy neural network. Firstly, fuzzy clustering is introduced to extract fuzzy membership functions and the fitness of fuzzy rules of T-S fuzzy neural network antecedent from the past processing data. Secondly, with the steepest descent method, back-propagation iteration is used to calculate the connection weights of the network. The processing of experiments shows that T-S fuzzy neural network modeling is superior to typical T-S model. The angle error and the straightness error processed by NTS-FNN is 40.4 %, 28.8 % lower than those of STS-FNN. The minimum processing time processed by NTS-FNN is lower by 46.1 % than that of STS-FNN