1 research outputs found
Switched allotype expression in an immunoglobulinânonsecreting rabbit lymphoid cell line fused with rabbit gangliocytes
The Simian virus 40âtransformed rabbit spleen cell TRSCâ1 synthesizes intracellular whole IgG molecules of the a1b4 allotype. Two hypoxanthineâguanine phosphoryl transferaseâdeficient mutants were derived from this line. One of these, TRSCâ1â8, was used in somatic cell fusion experiments together with gangliocytes from a rabbit immunized against ÎČâgalactosidase. Out of nineteen hybrid clones surviving in selective medium, only one, L17, was shown to produce free Îł chains which express the a2 allotype of the donor rabbit rather than the a1 marker of the parents TRSCâ1â8 line. The inability to restore IgG secretion in hybrids suggests that dominant regulatory controls are exerted by the TRSCâ1 genome on Ig production. This supports the notion that the TRSCâ1 line originated from a splenocyte that had not reached the final plasmocyte differentiation stage at the time of viral transformation. Copyright © 1981 WILEYâVCH Verlag GmbH & Co. KGaA, WeinheimSCOPUS: ar.jFLWNAinfo:eu-repo/semantics/publishe