Effects of ensiled Aspergillus oryzae and Saccharomyces cerevisiae cassava pulp as replacement for concentrate on ruminal fermentation in rumen-fistulated cows

Four experiments were conducted. I) The concentration of reducing sugar was determined following incubation with Aspergillus oryzae and an 8x11 factorial arrangement with 8 formulas of cassava components and 0 to 10 d of incubation. The reducing sugar increased from day 3 and the highest was found for 100% cassava pulp. II) Crude protein (CP) and urea content were determined after incubating with A. oryzae and Saccharomyces cerreviseae and a 4x6 factorial arrangement with 4 formulas of cassava components and 6 urea levels. The highest CP was observed in 37.5% cassava pulp, 25% cassava chip, and 37.5% cassava peel. III) The design was a 3x4 factorial arrangement with 3 formulas of cassava components and 4 urea levels. CP was unaffected by cassava components but increased with increasing urea levels. IV) The design was a 3x3 Latin squares with 3 fistulated cows and 3 periods. Treatments were 4 kg/cow/d concentrate, 3.2 kg/cow/d concentrate plus 0.8 kg/cow/d ensiled cassava pulp (ECP) and 2.4 kg/cow/d concentrate plus 1.6 kg/cow/d ECP. ECP increased pH, molar proportions of acetic and butyric acids but reduced molar proportion of propionic acid

Performance, Carcass Quality and Fatty Acid Profile of Crossbred Wagyu Beef Steers Receiving Palm and/or Linseed Oil

The objective of this study was to determine the effect of palm and/or linseed oil (LSO) supplementation on carcass quality, sensory evaluation and fatty acid profile of beef from crossbred Wagyu beef steers. Twenty four fattening Wagyu crossbred beef steers (50% Wagyu), averaging 640±18 kg live weight (LW) and approximately 30 mo old, were stratified and randomly assigned in completely randomized design into 3 treatment groups. All steers were fed approximately 7 kg/d of 14% crude protein concentrate with ad libitum rice straw and had free access to clean water and were individually housed in a free-stall unit. The treatments were i) control concentrate plus 200 g/d of palm oil; ii) control concentrate plus 100 g/d of palm oil and 100 g/d of LSO, iii) control concentrate plus 200 g/d of LSO. This present study demonstrated that supplementation of LSO rich in C18:3n-3 did not influence feed intakes, LW changes, carcass and muscle characteristics, sensory and physical properties. LSO increased C18:3n-3, C22:6n-3, and n-3 polyunsaturated fatty acids (PUFA), however, it decreased C18:1t-11, C18:2n-6, cis-9, trans-11, and trans-10,cis-12 conjugated linoleic acids, n-6 PUFA and n-6:n-3 ratio in Longissimus dorsi and Semimembranosus muscles

Effects of Linseed Oil or Whole Linseed Supplementation on Performance and Milk Fatty Acid Composition of Lactating Dairy Cows

The objective of this study was to determine the effects of linseed oil or whole linseed supplementation on performance and milk fatty acid composition of lactating dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The treatments consisted of basal ration (53:47; forage:concentrate ratio, on a dry matter [DM] basis, respectively) supplemented with 300 g/d of palm oil as a positive control diet (PO), or supplemented with 300 g/d of linseed oil (LSO), or supplemented with 688 g/d of top-dressed whole linseed (WLS). All cows were received ad libitum grass silage and individually fed according to the treatments. The experiment lasted for 10 weeks including the first 2 weeks as the adjustment period, followed by 8 weeks of measurement period. The results showed that LSO and WLS supplementation had no effects on total dry matter intake, milk yield, milk composition, and live weight change; however, the animals fed WLS had higher crude protein (CP) intake than those fed PO and LSO (p<0.05). To compare with the control diet, dairy cow’s diets supplemented with LSO and WLS significantly increased milk concentrations of cis-9, trans-11-conjugated linoleic acid (CLA) (p<0.05) and n-3 fatty acids (FA) (p<0.01), particularly, cis-9,12,15-C18:3, C20:5n-3 and C22:6n-3. Supplementing LSO and WLS induced a reduction of medium chain FA, especially, C12:0-C16:0 FA (p<0.05) while increasing the concentration of milk unsaturated fatty acids (UFA) (p<0.05). Milk FA proportions of n-3 FA remarkably increased whereas the ratio of n-6 to n-3 decreased in the cows supplemented with WLS as compared with those fed the control diet and LSO (p<0.01). In conclusion, supplementing dairy cows’ diet based on grass silage with WLS had no effect on milk yield and milk composition; however, trans-9- C18:1, cis-9, trans-11-CLA, n-3 FA and UFA were increased while saturated FA were decreased by WLS supplementation. Therefore, it is recommended that the addition 300 g/d of oil from whole linseed should be used to lactating dairy cows’ diets

Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker

This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control. The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h was approximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and their eggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISA technique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p <0.05). Based on our results, AChE could be applied to assess cypermethrin exposure in the snails and their eggs, in order to plan contamination management of such pesticides in the snails and reduce the risks to consumers

Milk Production, Milk Composition, Live Weight Change and Milk Fatty Acid Composition in Lactating Dairy Cows in Response to Whole Linseed Supplementation

The objective of this study was to determine the effects of whole linseed supplementation on performances and milk fatty acid composition of dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The control group received 300 g of palm oil. The second group was supplemented with 344 g/d of top-dressed whole linseed plus 150 g of palm oil and the third group was supplemented with 688 g/d of top-dressed whole linseed. All cows also received ad libitum grass silage (Brachiaria ruziziensis), had free access to clean water and were individually housed in a free-stall unit and individually fed according to treatments. Residual feeds were collected on 2 consecutive days weekly and at the end of the experiment. Feed samples were pooled to make representative samples for proximate and detergent analyses. Daily milk yields were recorded. Milk samples were collected on 2 consecutive days weekly. Live weights were recorded at the start and at the end of the experiment. Milk samples were taken on d 56 of the experiment and subjected to milk fatty acid composition. The results showed no statistical significant differences in intakes, live weight change, milk yields and milk compositions, however, C18:1, C18:3 and unsaturated FAs were increased while saturated FAs were reduced by whole linseed supplementation. It is recommended that the addition of 300 g/d oil from whole linseed could be beneficial to lactating dairy cows in early lactation

Performance, carcass quality and fatty acid profile of crossbred brahman beef steers receiving palm or rice bran oil

Twelve crossbred Brahman steers were randomly stratified into 3 treatment groups to determine the effect of palm or rice bran oil supplementation on the performance and fatty acid profile in beef. The steers averaged 300±38 kg live weight and 18±3 months old. All steers were fed 6 kg/d of 12% crude protein concentrate with ad libitum rice straw. The treatments were 1) control concentrate, 2) control concentrate plus 200 g/d of palm oil (PO), and 3) control concentrate plus 200 g/d of rice bran oil (RO). The study demonstrated that supplementation of PO or RO did not influence carcass and muscle characteristics or sensory and physical properties. However, PO and RO significantly increased net energy intake, C16:0, C18:1n-9, monounsaturated fatty acids, and total fatty acid intakes. Beef marbling scores also increased significantly by PO or RO supplementation. The C18:2n-6, trans10, cis12 C18:2 CLA, and polyunsaturated fatty acids were significantly higher in cattle on RO than PO