Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Rilpivirine Hydrochloride in Tablet Dosage Form

A simple, sensitive, rapid and reproducible HPLC Method was developed and validated for estimation of Rilpivirine in the presence of degradation products generated from forced decomposition studies. The analysis was carried out on Hypersil BDS C18, 250 X 4.6mm, 5? column using a mixture of ammonium acetate Buffer (pH to 6.0 0.05) and Acetonitrile in the proportion 55:45 respectively as a mobile phase at a flow rate of 1.2 mL/minute. The wavelength selected for the analysis was 300 nm. The peak for Rilpivirine HCl was observed at 10.33 minute. A linear response was observed in the range of 12.5 - 62.5 ?g/mL with a correlation coefficient of 0.999. The method was validated for specificity, linearity, precision, accuracy and robustness. The obtained results were indicating that the method is selective in analysis of Rilpivirine in the presence of degradation products formed under various stress conditions

DEVELOPMENT OF A DENSITOMETRIC HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE ANALYSIS OF URSOLIC ACID IN THE LEAVES OF SPECIES OF GENUS TECOMA AND TABEBUIA OF BIGNONIACEAE FAMILY

Objective: A simple and sensitive high-performance thin–layer chromatographic (HPTLC) method was developed for the quantification of ursolic acid in the leaves of three species of genus Tecoma (Tecoma gaudichaudi DC, Tecoma capensis (Thunb.) Lindl, Tecoma stans (L.) Juss. ex Kunth) and genus Tabebuia (Tabebuia rosea Bertol) belong to family Bignoniaceae.Methods: Chromatography was performed on Silica gel 60 F254 precoated HPTLC Plates with optimised mobile phase pet ether: ethyl acetate: formic acid (7:3:0.5, v/v/v). The plate was derivatized with p-anisaldehyde reagent and scanned at 540 nm. The developed method was found to give a compact spot for ursolic acid at Rf value 0.43±0.01. The method was validated using International Council for Harmonization (ICH) guidelines, including linearity, precision, accuracy, and robustness.Results: Ursolic acid was found to be present in four species, i.e., Tecoma gaudichaudi DC (1.48%w/w), Tecoma capensis (Thunb.) Lindl. (0.79%w/w), Tecoma stans (L.) Juss. Ex Kunth (1.11%w/w), Tabebuia rosea (Bertol.) (1.13%w/w). A good linearity relationship was found to be (200-1400ng band−1) with a correlation coefficient (r2) value of 0.9946 with ursolic acid. Limit of detection and limit of quantification was considered to be 40.66, 123.21 ng per band respectively. The developed method was found to be accurate and precise with 1.32%, 1.19% (%RSD) for interday and intraday precision. The accuracy of the method was performed by recovery studies at three different concentration levels, and the average percentage recovery was found to be 98.05% for ursolic acid.Conclusion: The proposed method for the quantitation of ursolic acid was found to be reproducible and simple

Effect of Different Extraction Conditions on Total Alkaloids, Total Phenolic and Total Flavonoid Content of Vigna mungo L hepper

Vigna mungo L Hepper is an important pulse crop cultivated in India. It is rich source of many nutritional compounds and it is an integral part of diet. Bioactive compounds like phenolic, alkaloids, flavonoids, saponins etc. are present in the seeds. Objective is to determine the content of bioactive compounds present in the black gram seeds and to study effect of sample pretreatment, fractionation on total alkaloid, total phenolic and total flavonoids content. Three types of extracts were prepared by using ethanol as a solvent by using Soxhlet extractor. First Ethanolic extract (EE), second extract prepared from pretreated seeds with an acid (EEH) and third fraction from ethanolic extract (EF) were prepared. Total alkaloid content were determined spectrophometrically by using Bromocresol green using Atropine as a standard. Total phenolic content was estimated spectrophometrically by using Gallic acid as a standard. Quercetin was used as a standard for estimation of total flavonoids content. The total phenolic content of the EE, EF and EEH extract, was 20.0 ± 5.28, 21.03±5.04 and 17.8±5.77 Gallic acid equivalents/g respectively. The total flavonoid content of EE, EF and EEH extract was 166.7± 3.66, 304.2 ±3.48 and 112.5± 3.95 quercetin equivalents/g. The total alkaloid content of EE, EF and EEH extract, was 121.9±3.77, 154.8±3.60 and 202.1±3.49 Atropine equivalents/g. various treatments have effect on extraction of bioactive compounds. Extract from pretreated seeds with acid improved extraction of alkaloids. Fractionation of extract yield higher content of flavonoids and phenolic content than normal ethanolic extract. Hydrolysis of extract results in decreased concentration of flavonoids and phenolic. Keywords: Alkaloid, Bioactive, Flavonoids, Phenolic, Vigna mung

An Insight on Analytical Profile on Bisoprolol Fumarate – A Selective Beta-1 Adrenoreceptor Blocker

BF is Beta-adreno receptor antagonist and used as an AntiHypertensive Drug. BF gives the blocking action on β1-adrenergic receptors in the heart and vascular smooth muscle. The present review compiles the various approaches implemented for quantification of BF in bulk drug, pharmaceutical matrix and biological fluid. This review represents more than 50 analytical methods which include capillary electrophoresis, HPLC, HPTLC, UV-Spectroscopy, UPLC, impurity profiling and electrochemical methods implemented for estimation of BF as a single component as well as in multicomponent

WITHDRAWN: Recent advances in compression-coated tablets as a controlled drug delivery system

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause.The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy

Formulation and In Vitro Evaluation of Sun Protection Factor of Herbal Sunscreen Cream Containing Butea monosperma, Neolamarckia cadama and Punica granatum Extracts

Sun radiations are the primary source of light & energy and the main causative factor in various skin conditions such as sunburn, photoaging, skin cancer development.These harmful effects may be due to the production of free radicals(Reactive oxygen species). Physical sun protection is not sufficient to get rid of such unwanted changes skin appearance and physiology. Now a day’s cosmetics and pharmaceuticals are added with natural sunscreens protecting reagents to provide health benefit apart from beautification. The aim of the present study is to formulate and evaluate natural sun protective topical formulation comprising methanolic extract of Butea monosperma (Lam.) flower, Neolamarckia cadama (Roxb.) leaves and Punica granatum (Linn) peel. The formulated cream was evaluated for its Total flavonoid and phenolic content, In vitro sun protecting activity and physicochemical parameters. The Total flavonoid and Phenolic content were found to be 42.07  mg QE/g and 57.03 ± 3.06 mg GAE/g respectively. While the SPF values were found to be 1.75,2.94,3.87 for the concentration 20 µg/ml, 30 µ g/ml and 40 µ g/ml respectively. From the study we can conclude that cream containing herbal extracts will enhance the sun protection property and it will significantly contribute to UV absorbing property of conventional herbal sunscreen formulations. It will provide the great advantage of avoiding the adverse and undesirable effects pf synthetic compounds. Keywords: Butea monosperma (Lam.),Neolamarckia cadama (Roxb.), Punica granatum (Linn), sun protection factor and antioxidant .&nbsp

A Review Bioactive Components of Vigna mungo

The bioactive compound is secondary plant metabolites eliciting pharmacological or toxicological effects in man and animals. Legumes are a valuable source of proteins and nutrients for the majority of the world population. Vigna mungo is one of the important legume crops extensively cultivated in India and other parts of the world. Pulses and legumes have been gaining interest because they are an excellent source of bioactive compounds. The objective of this present review is to compile all relevant information published regarding bioactive components from the Vigna mungo. Various bioactive components reported in Vigna mungo were found and it includes flavonoids, isoflavonoids, phytoestrogens, phenolic acids, enzymes, fibers, starches, trypsin inhibitors, phytic acid, lectins, saponins, tocopherols, fatty acids and proteins. This review clearly demonstrates that Vigna mungo is rich in bioactive components and these components are located in various organs of the plant

Investigation on effects of precursor pre-heat treatments on CIGS formation using spin-coated CIG precursor

A low cost, non-vacuum process involving spin coating of metallic precursors followed by selenization was substantiated for the fabrication of CIGS (CuInxGa(1−x)Se2) thin film absorber. Spin coating of CIG precursors using environmentally benign solvents and studying the effects of various heat treatments on the spin-coated CIG precursor film leads to high-quality crystalline CIGS thin films with minimum carbon impurities. In this work, effects of various heat treatments of CIG precursor (heat treatment of precursor films after each individual run of spin coating using hot plate, using air dryer, without any pre-heat treatment and heating of 30-run coated sample on hot plate for 30 mins) followed by selenization has been investigated and being reported. The as-prepared CIGS thin film samples are characterized by X-ray diffraction (XRD), Raman spectroscopy (RS), Field emission scanning electron microscopy (FESEM), UV-Vis-IR spectroscopy and X-ray photo electron spectroscopy. From XRD pattern and Raman spectra, MoSe2 phase is also observed along with tetragonal chalcopyrite CIGS phase. Through FESEM and EDS, it is observed that heating each individual run has resulted a high-quality compact dense film with minimum carbon composition. XPS also confirms the minimum carbon composition in case of preheating individual run. From UV-Vis spectra, the bandgap of the prepared CIGS material is found to be 1.5 eV, essential for the cell fabrication

RECENT RESEARCH ON ANALYTICAL METHODS OF ANALYSIS OF RALTEGRAVIR AND ELVITEGRAVIR : A REVIEW

ABSTRACT Highly Active antiretroviral therapy ( HAART ), a combination drug therapy is a topic of current interest in the treatment of HIV and AIDS. Techniques for the analysis and the quality control of antiretroviral drugs, particularly in the drug combinations are vital in achieving quality of these drugs and the treatments involved. Integrase inhibitor are a class of antiretroviral drug designed to block the action of integrase , a viral enzymes that inserts a viral genome into the DNA of the host cell. Since integration is a vital step in retroviral replication, blocking it can halt further spread of the virus. Integrase inhibitor were initially developed for the treatment of HIV infection . The HPLC,UV and HPTLC methods are available for the analysis of Raltegravir and Elvitegravir, the recently used drug for HIV and AIDS are reviewed in this articles

Synthesis and Antimicrobial Activity of Some New 1, 4-Benzothiazine Containing Thiosemicarbazides and 1, 3, 4-Oxadiazole Derivatives

A series of novel 3- methyl-7-substituted-4H,4-benzothiazine-2-carbohydrazide (3a-e) and corresponding thiosemicarbazides (4-a-q); 2-[3-methyl-7-substituted- 4H -1, 4-benzothiazine-2-yl]-N-(aryl) hydrazine carbothiamide have been synthesized. The thiosemicarbazide when cyclized with iodine via intramolecular cyclisation gave benzothiazonyl oxadiazoles (5-a-q); 5-(3-methyl -7-substitued-4H- 1,4-benzothiazin-2-yl)- N —aryl- 1,3,4- oxadiazol -2-amine and the compounds were tested for antibacterial and antifungal activities against different microorganisms