Husbandry practices and gut health outcomes in weaned piglets: A review

The immediate post-weaning period is one of the most stressful phases in a pig's life, and during this period, piglets are usually exposed to environmental, social and psychological stressors which have direct or indirect effects on gut health and overall growth performance. In this review, the impact of husbandry practices on gut health outcomes and performance of piglets is discussed. Husbandry practices in the swine barn generally include nutrition and management practices, maintenance of hygienic standards and disease prevention protocols, and animal welfare considerations. Poor husbandry practices could result in reduced feed intake, stress and disease conditions, and consequently affect gut health and performance in weaned piglets. Reduced feed intake is a major risk factor for impaired gut structure and function and therefore a key goal is to maximize feed intake in newly weaned piglets. In weaned piglets, crowding stress could reduce pig performance, favor the proliferation of pathogenic bacteria resulting in diarrhea, stimulate immune responses and interfere with beneficial microbial activities in the gut. Sanitation conditions in the swine barn plays an important role for optimal piglet performance, because unclean conditions reduced growth performance, shifted nutrient requirements to support the immune system and negatively affected the gut morphology in weaned piglets. Appropriate biosecurity measures need to be designed to prevent disease entry and spread within a swine operation, which in turn helps to keep all pigs and piglets healthy. Collectively, husbandry practices relating to feeding and nutrition, animal welfare, biosecurity and disease prevention are important determinants of gut health and piglet performance. Thus, it is suggested that adopting high husbandry practices is a critical piece in strategies aimed at raising pigs without the use of in-feed antibiotics

Impact of rare earth element-enriched yeast on growth performance, nutrient digestibility, blood profile, andfecal microflora in finishing pigs

This study was conducted to determine effects of rare earth element-enriched yeast (RY) on growth performance, nutrient digestibility, blood profile, and fecal microbial flora in finishing pigs. A total of 100 finishing pigs [(Landrace Ă Yorkshire) Ă Duroc] were used in a 2-phase feeding trial. Pigs were randomly assigned to 5 treatments according to their initial body weight (5 pens per treatment, 2 gilts and 2 barrows per pen). Treatments were: basal diets, free of antibiotics [negative control (NC)]; NC + 500, 1000, and 1500 mg/kg, respectively, of RY; antibiotics diets, NC + 500 mg/kg of tiamulin [positive control (PC)]. Overall, average daily gain (ADG) and gain-to-feed ratio (G:F) increased linearly with increasing level of RY in diets. At the end of the experiment, blood lymphocyte concentration and apparent total tract digestibility of dry matter and gross energy increased linearly in pigs fed the increasing level of RY in diets. The better ADG, G:F, and fecal Lactobacillus counts were observed in both RY and PC groups compared with the NC group. In conclusion, diets with RY improved growth performance, digestibility, blood lymphocyte counts, and fecal lactobacillus counts in finishing pigs, also, RY may be considered as an alternative to antibiotics.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Standardized ileal digestible amino acid contents in flaxseed and flaxseed meals fed to growing-finishing pigs

Ten cannulated barrows with an average body weight of 55.99 3.73 were assigned to test diets containing either full-fat flaxseed (FFS), expeller-pressed flaxseed meal (expeller-FM), or solvent-extracted flaxseed meal (solvent-FM). The SID of Met and Thr was higher (PThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Pigs weighing less than 20 kg are unable to adjust feed intake in response to dietary net energy density regardless of diet composition

This study was conducted to investigate the effects of increasing dietary net energy (NE) density manipulated by either dietary fat or fibre content on growth performance and energy intake in weaned pigs. Sixty barrows (8.40 0.91 kg) were randomly allotted to 1 of 5 dietary treatments based on initial body weight. The experimental diets contained increasing NE densities (i.e. 9.9, 10.3, and 10.7 MJ NE/kg) by manipulating either dietary fat or fibre content. Feeding the different dietary treatments did not affect growth performance among dietary treatments. The apparent total tract digestibility of dry matter, gross energy, fat, and neutral detergent fiber of the diets linearly increased (PThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Dose-response effects of in-feed antibiotics on growth performance and nutrient utilization in weaned pigs fed diets supplemented with yeast-based nucleotides

Dietary nucleotides are bioactive compounds with the potential to mitigate weaning-associated challenges in piglets. An experiment was conducted to determine the interaction effect of antimicrobial growth promoters (AGP) and a nucleotide-rich yeast extract (NRYE) on growth performance and apparent total tract digestibility (ATTD) of dry matter (DM), crude protein (CP) and gross energy (GE), and to establish whether NRYE supplementation may completely or partially replace AGP in diets for weaned pigs. In phase 1 and 2, corn, wheat, canola meal and soybean meal based diets, which were formulated to contain 0.0 or 0.1% NRYE with 0, 25, 50, 75 or 100% of the recommended AGP dosage, were fed to 108 twenty-one day old piglets (initial body weight 7.11 ± 0.9 kg; mean ± SD) from d 1 to 14 and 15 to 28, respectively. Overall, increasing AGP level in NRYE supplemented diets linearly decreased average daily gain (ADG) (P = 0.002) and gain-to-feed ratio (G:F) (P = 0.007); and quadratically decreased ATTD of DM (P = 0.001), CP (P = 0.003) and G:F (P = 0.017) during phase 2. Compared with control and pigs fed NRYE with 100% of recommended AGP dosage, pigs fed 0.1% NRYE without AGP had greater (P < 0.05) ADG and G:F in phase 2 and overall. In conclusion, supplementing 0.1% NRYE improved growth performance of pigs but this beneficial effect was reduced by increasing dietary AGP dosage. Keywords: Antimicrobial growth promoters, Digestibility, Growth performance, Piglet, Nucleotide-rich yeast extrac

Effect of dietary organic acids supplementation on growth performance, nutrient digestibility, and gut morphology in weaned pigs

This study was conducted to investigate the effects of dietary supplementation with different organic acid (OA) mixtures on growth performance, nutrient digestibility, and gut morphology in weaned pigs. A total of 56 weaned pigs (7.93 1.04 kg body weight) were assigned to four dietary treatments with seven replicates per treatment for a 35-d study conducted over two phases; phase 1 (d 1 to 14) and phase 2 (d 14 to 35). Diets consisted of 1) a corn-soybean meal-basal diet without any additive (negative control, NC); 2) NC + formic and propionic acids (TRT1); 3) NC + butyric, formic, and propionic acids (TRT2); and 4) NC + antibiotic (positive control, PC). During the overall period, the gain to feed ratio of pigs fed the PC and TRT1 diets tended to be higher (P = 0.059) than that of the NC diet. However, OA supplementation had no effects on nutrient digestibility. Jejunal villus height to crypt depth ratio was higher (PThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Growth performance and expression of genes encoding enzymes involved in methionine and cysteine metabolism in piglets fed increasing sulphur amino acid to lysine ratio during enterotoxigenic Escherichia coli challenge

A study was conducted to examine the effect of standardised ileal digestible (SID) sulphur amino acids/lysine ratio (SAA/Lys) on performance and expression of methionine adenosyltransferase 1 and 2 alpha (MAT1α and MAT2α), and cystathionine gamma-lyase (CTH) in piglets challenged with an enterotoxigenic Escherichia coli (ETEC). Thirty five [Duroc × (Yorkshire × Landrace)] piglets (6.9 ± 0.5 kg) were randomly assigned to five dietary treatments. The diets were antibiotic free with SID SAA/Lys of 48%, 54%, 60%, 66%, and 72%. Pigs were orally challenged with 6 and 15 mL of ciprofloxacin-resistant ETEC K88+ on days 7 and 10. Blood samples were collected before (BC) and 6, 24, and 48 h after challenge (AC). Body weight gain and feed intake were collected on days 0, 6, and 12 to determine average daily gain (ADG). Gain to feed ratio (G/F) was calculated by dividing ADG by average daily feed intake (ADFI). On day 13, all pigs were euthanized to collect liver and ileal samples to analyse gene expression using real-time polymerase chain reaction. Pigs fed the diet containing SAA/Lys of 66% had the highest ADG, ADFI, and G/F BC. However, ADG, ADFI, and G/F were similar across all ratios AC. Serum tumor necrosis factor alpha concentration at 6 h AC was higher (P < 0.05) than BC and was improved with increasing SAA/Lys. Increasing SAA/Lys quadratically increased (P < 0.01) CTH and MAT1α expression. Ileal expression of CTH and MAT2α were quadratically increased (P < 0.05) with increasing SAA/Lys. In conclusion, SAA/Lys of 60% is suggested to be optimum for piglets to tolerate ETEC pathogenic challenge.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Short-term effect of supplemental yeast extract without or with feed enzymes on growth performance, immune status and gut structure of weaned pigs challenged with Escherichia coli lipopolysaccharide

Abstract Background This study investigated the response of piglets receiving a yeast extract without or with a multi-enzyme mixture compared with an antimicrobial growth promoter (AGP) on performance, immune status and gut structure after an E. coli lipopolysaccharide (LPS) challenge. Thirty-six pigs were allotted to six treatments including: a non-challenged control (NCC); LPS-challenged control (CC); CC + AGP; CC + yeast extract; CC + enzymes; and CC + enzymes + yeast extract. On d 7, pigs were bled and thereafter injected with LPS or sterile saline. Blood samples were collected at 6, 48, and 96 h post-challenge. After 96 h post-challenge, pigs were euthanized to obtain duodenal, jejunal and ileal samples. Results Overall (d 1 to 11), compared with CC pigs, AGP attenuated the LPS-induced reduction in ADG (P = 0.004), ADFI (P = 0.03) and gain/feed ratio (P = 0.01). At 6 h post-challenge, AGP pigs had lower plasma urea N (PUN; P = 0.02) and serum TNF- α concentration (P = 0.07), and higher platelet count (P = 0.04) and serum IL-10 concentration (P = 0.02) than CC pigs. At 48 h post-challenge, AGP pigs had lower PUN (P = 0.02) than CC pigs, whereas enzymes + yeast extract interacted non-additively (P = 0.001) to reduce PUN. At 96 h post-challenge, AGP pigs had lower PUN (P = 0.02) and higher duodenal (P = 0.03), jejunal (P = 0.01) and ileal (P = 0.07) villus height than CC pigs. In addition, enzymes + yeast extract interacted additively and non-additively to reduce ileal IFN-γ (P  0.10) were observed between AGP and enzymes + yeast extract pigs on other measured parameters except for the downregulation of ileal IFN-γ (P < 0.0001) and TNF-α (P = 0.003) in enzymes + yeast extract pigs at 96 h post-challenge. Conclusions The LPS challenged piglets receiving enzymes + yeast extract showed beneficial responses in gut structure and immunity commensurate with those receiving antibiotics, though the latter had better overall growth performance

Performance and Nutrient Utilization Responses in Broilers Fed Phytase Supplemented Mash or Pelleted Corn-Soybean Meal-Based Diets

Effect of phytase produced by thermo-protective coating technology (coated phytase) on performance and nutrient utilization of broiler chicks fed a corn-soybean meal-based pelleted diet was investigated. A total of 144-one-day-old male broiler (Ross) chicks were divided in 24 groups and fed 4 diets (6 groups per diet) for 21 days. The diets included a mash or pelleted basal diet without or with the coated phytase at 500FTU/kg in a 2&times;2 factorial arrangement. Calcium and non-phytate P values in the basal diet were 22 and 51% lower compared with NRC (1994) recommended values, respectively. All the diets contained titanium oxide as an indigestible marker. There was no interaction (P&gt;0.05) between diet form and phytase on any of response criteria measured in this study except body weight gain and feed intake such that phytase supplementation improved (P&lt;0.05) these 2 response criteria, but improvements were greater for pelleted diet than mash diet. Phytase supplementation increased (P&lt;0.05) tibia ash and ileal P digestibility, by 10.5 and 38.1%, respectively. Phytase supplementation did not, however, affect (P&gt;0.05) the ileal digestibilities of Ca, crude protein and amino acids. In conclusion, coated phytase supplementation to corn-soybean meal-based diet for broilers resulted in improved growth performance, bone mineralization and P digestibility regardless of diet type (mash versus pellet). These findings suggest that the efficacy of the coated phytase used in the current study was not influenced by the pelleting process