DETERMINATION OF ATORVASTATINE IN PHARMACEUTICAL FORMULATIONS BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A simple, sensitive and reproducible reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative estimation of Atorvastatin calcium (ATOR-C) in the pharmaceutical formulations. Chromatographic separation was achieved on a 250 4.6 mm, 5?, Waters symmetry column. The flow rate was 1mL/min and eluent was monitored by absorbance at 246.0 nm using a mixture of Methanol and Acetonitrile (pH 3.00.01) in the ratio of 25:75 (v/v). The retention times of ATOR-C was found to be 5.5 min. Calibration plots were linear in the concentration range of 5-25 ?g/mL for ATOR-C calcium. The total run time was 12 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday, intraday, analyst and instrument precision), robustness and LOD/LOQ values and it was successfully employed for the determination of ATOR-C in pharmaceutical tablet formulations

An Analysis of Resistance Spot Weld Growth on Mild and Stainless Steel with 1mm and 2mm Thicknesses

Resistance spot welding (RSW) is an essential welding technology today for joining two or more metals in various manufacturing industries. A statistic shows that one metal assembly out of five is joined using resistance spot welding mechanism, commercially.   It uses traditionally two electrodes to hold the metal sheets and forces high current to pass through it. The growth of weld nugget is, at last obtained from a proper set up of its controlling parameters such as current, weld time, pressure of electrodes and also the tip size of electrodes. However, factors such as electrode deformation, dissimilar materials and materials with different thicknesses also affect weld growth. This paper looks into the effects of different thicknesses of two base materials .The materials that were used are mild steel and 302 authentic stainless steel with thicknesses of 1 mm and 2 mm. Mechanical tensile test and hardness test have been carried out to characterize the formation of weld nugget growth for different welding schedules. The results of the experiments showed that the growth of spot weld is strongly affected by the usage of materials with different thickness or types. The macrostructure of weld nugget also shows distinguishable differences in weld growth for the both mentioned cases. The tensile test was carried out on standard size samples but with different thicknesses and materials. It shows difference in yield strength for the same welding schedules. Meanwhile the hardness of welded materials varies from one another significantly but the hardness distribution along the welded areas seemed to almost same for each category of base metals

Development of validated RP-HPLC method for the simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form

A RP-HPLC method for the estimation of ATN (Atenolol) and CTN (chlorthalidone) in combined dosage form was developed using Comosil RP-C18 (4.6 x 250mm, 5m) in an gradient mode with mobile phase comprising of Methanol: Water (pH 3 using OPA) The flow rate was 1 mL/ min and effluent was monitored at 226.0 nm. The retention times were found to be 2.2 min for ATN and 3.36 min for CTN. The assay exhibited a linear dynamic range of 40- 200 g/mL for ATN and 10- 50 g/mL for CTN. The calibration curves were linear (r 2 = 0.999 for ATN and r 2 = 0.999 for CTN) over the entire linear range. Mean % recovery was found to be 99.78 % for ATN and 99.30 % for CTN with % RSD was NMT 2 for both estimations which fully agrees with system suitability which is in good agreement with labeled amount of formulation. The % RSD for Intra- Day and Inter-Day Precision was NMT than 2 for both the drugs. The developed method was validated as per ICH guideline

EVALUATION OF ANTI-ASTHMATIC ACTIVITY OF METHANOLIC EXTRACT OF BERLERIA PRIONITIS LINN. AERIAL PARTS

Objective: The aim of the present study is to evaluate the aerial parts of a plant of Barleria prionitis Linn., for its antiasthmatic activity with the separation of active moieties.Methods: Adult wistar albino rats were used for the anti-inflammatory study. Histamine-induced bronchospasm was conducted on isolated goat trachea.Results: The dried and powdered aerial parts of Berleria prionitis was extracted with continuous soxhlet extraction with Petroleum ether (40-60 ° C), Chloroform, Ethyl acetate, Acetone, Methanol, and Hydroalcoholic solvents. Preliminary phytochemical screening of all extracts was done. Preliminary animal studies by In vitro isolated goat trachea chain preparation of all extracts were done to find out the potent extract. In this study, the methanolic extract of aerial parts of Berleria prionitis was found to be potent comparative to another extract. The results of carrageenan induced rat paw oedema model indicated the dose-dependant anti-inflammatory activity. As compared to standard drug (Indomethacin), methanolic extract showed similar activity which was found to be statistically significant (P<0.0001). The extent of DPPH radical scavenging was determined by calculated the IC50 value of methanolic extract Berleria prionitis (133.5) compared with the Ascorbic acid (114.7) taken as standard. In the present study, the histamine-induced dose-dependent contraction of goat tracheal chain was significantly inhibited (p<0.001) by methanolic extract of aerial parts of Berleria prionitis (200 μg/ml). Thus the present study revealed that the methanolic extract of Berleria prionitis (MEBP) has significant antihistaminic (H1 receptor antagonist) activity.Conclusion: In view the fact that tribal have well experienced the antiasthmatic effects of the roots of Barleria prionitis Linn. The results of our study, for the first time, show that the methanolic extract of aerial parts of Berleria prionitis Linn. possesses antioxidant, anti-inflammatory, Bronchodilator properties and therefore can be used for the antiasthmatic treatment

Comparative thermal performance of static sunshade and brick cavity wall for energy efficient building envelope in composite climate

Energy efficient building technologies can reduce energy consumption in buildings. In present paper effect of designed static sunshade, brick cavity wall with brick projections and their combined effect on indoor air temperature has been analyzed by constructing three test rooms each of habitable dimensions (3.0 m × 4.0 m × 3.0 m) and studying hourly temperatures on typical days for one month in summer and winter each. The three rooms have also been simulated using a software and the results have been compared with the experimental results. Designed static sunshade increased indoor air temperature in winter while proposed brick cavity wall with brick projections lowered it in summer. Combined effect of building elements lowered indoor air temperature in summer and increased it in winter as compared to outdoor air temperature. It is thus useful for energy conservation in buildings in composite climate

DETERMINATION OF ATORVASTATIN CALCIUM IN PHARMACEUTICAL FORMULATIONS BY REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A simple, sensitive and reproducible reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative estimation of Atorvastatin calcium in the pharmaceutical formulations. Chromatographic separation was achieved on a 250 × 4.6 mm, 5μ, Waters symmetry column. The flow rate was 1ml/min and eluent was monitored by absorbance at 246 nm using a mixture of Methanol and Acetonitrile (pH 3.

Review: Ion mobility spectroscopy a new method of analysis, its application and reproducibility problems

Ion Mobility Spectrometry (IMS) is a mass-selective technique in which the sample is vaporised, ionised, and with given initial velocity moved along a drift region towards a collector electrode. The drift times (milliseconds, ms) needed by the ions to reach the collector are proportional to their masses: the higher the mass, the longer the drift times. During our evaluation of ion mobility spectrometry in the screening it was found that reproducibility problem may occurred which is solve easily by using some methods, described in this review. Ion mobility spectroscopy is widely use for the drug analysis, hair analysis and other applications

DETERMINATION OF TELMISARTAN AND FORCED DEGRADATION BEHAVIOR BY RP-HPLC IN TABLET DOSAGE FORM

A simple, rapid, precise, rapid, sensitive and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method for determination of Telmisartan in tablet dosage form was developed and validated. Chromatographic separation was achieved on a 250 × 4.6 mm, 5μ, Waters symmetry column in gradient mode, with mobile phase consisting of a mixture of solution (10 mM potassium dihydrogen phosphate, pH 3.

STABILITY-INDICATING RP-HPLC METHOD FOR ANALYSIS OF TELMISARTAN IN THE DOSAGE FORM

A simple, rapid, precise, rapid, sensitive and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for quantitative analysis of Telmisartan (TELM) in pharmaceutical dosage forms. Chromatographic separation of TELM and its degradation products was achieved on a C18, 250 × 4.6 mm, 5μ, Waters symmetry column