Fast fluorescence dynamics in non-ratiometric calcium indicators

A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.Comment: 3 pages, 2 figures, figures revised, added chi-square goodness of fi

Reduced order models of integrated RF spiral inductors with geometrical and technological automatic parameterization

In this paper a method is presented for automatically generating a parameterized model of integrated inductors accounting for geometry and substrate effects. A multiparameter Krylov-subspace based moment matching method is used to reduce the three-dimensional integral equations describing the EM behavior of the inductor over the substrate. Parameterization enables optimization of geometry and substrate technology simultaneously