BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES AND THEIR SYNERGISTIC EFFECT WITH ANTIBIOTICS: A STUDY AGAINST SALMONELLA SP

Objective: In recent era, medical science has turned to a variety of novel products and technologies to halt the spread of infections. The most promising and effective antimicrobial agents being embraced by modern medical science today is silver. With increasing drug-resistance and growing concern regarding the over-prescribing of antibiotics, there has been a resurgent interest in the use of antimicrobial silver. Unlike antibiotics, silver appears to be immune to resistance. Thus, the conjugation of antibiotic with silver nanoparticles would prevent development of resistance of microbes and increase the antimicrobial property of the antibiotic.Methods: In this manuscript, extracellular biological synthesis of silver nanoparticles was made from Trametes sp. The characterization of the nanoparticles was carried out as well as its antibiotic efficacy was evaluated in addition to the antibiotic combination.Results: Initially the fungal cell filtrate was challenged with 1Mm AgNO3 solution, in a 250 ml Erlenmeyer flask and agitated at 30 °C in dark for 72 h under static conditions at 200 rpm and the silver nanoparticle production was monitored by the change in color and later it was subjected to optical measurements by UV-Vis spectrophotometer. The characters of these silver nanoparticles were further studied by Fourier Transform Infrared (FTIR) Spectroscopy, SEM and TEM studies. The nanoparticles produced during the study period were found to have wider antibacterial property and also it showed the enhanced efficacy in combination with ceftriaxone and ofloxacin against Salmonella sp.Conclusion: The synergistic mode of antibiosis in between nanoparticles synthesized from Trametes sp. and ceftriaxone, ofloxacin was found to be more effective against Salmonella sp. in the current study.Â