A Smartphone APP for Health and Tourism Promotion

The main purpose of this study is to develop an APP by integrating GPS to provide the digitized information of local cultural spots to guide tourists for tourism promotion and the digitized information of mountaineering trails to monitor energy expenditure (EE) for health promotion. The provided cultural information is also adopted for educational purpose. Extended Technology Acceptance Model (TAM) was used to evaluate the usefulness and behavior intention of the provided information and functions in the developed system. Most users agreed that the system is useful for health promotion, tourism promotion, and folk-culture education. They also showed strong intention and positive attitude toward continuous use of the APP

Highly reliable GIGA-sized synthetic human therapeutic antibody library construction

BackgroundMonoclonal antibodies (mAbs) and their derivatives are the fastest expanding category of pharmaceuticals. Efficient screening and generation of appropriate therapeutic human antibodies are important and urgent issues in the field of medicine. The successful in vitro biopanning method for antibody screening largely depends on the highly diverse, reliable and humanized CDR library. To rapidly obtain potent human antibodies, we designed and constructed a highly diverse synthetic human single-chain variable fragment (scFv) antibody library greater than a giga in size by phage display. Herein, the novel TIM-3-neutralizing antibodies with immunomodulatory functions derived from this library serve as an example to demonstrate the library’s potential for biomedical applications.MethodsThe library was designed with high stability scaffolds and six complementarity determining regions (CDRs) tailored to mimic human composition. The engineered antibody sequences were optimized for codon usage and subjected to synthesis. The six CDRs with variable length CDR-H3s were individually subjected to β-lactamase selection and then recombined for library construction. Five therapeutic target antigens were used for human antibody generation via phage library biopanning. TIM-3 antibody activity was verified by immunoactivity assays.ResultsWe have designed and constructed a highly diverse synthetic human scFv library named DSyn-1 (DCB Synthetic-1) containing 2.5 × 1010 phage clones. Three selected TIM-3-recognizing antibodies DCBT3-4, DCBT3-19, and DCBT3-22 showed significant inhibition activity by TIM-3 reporter assays at nanomolar ranges and binding affinities in sub-nanomolar ranges. Furthermore, clone DCBT3-22 was exceptionally superior with good physicochemical property and a purity of more than 98% without aggregation.ConclusionThe promising results illustrate not only the potential of the DSyn-1 library for biomedical research applications, but also the therapeutic potential of the three novel fully human TIM-3-neutralizing antibodies

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Luminance coding in graph-based representation of multiview images

Multi-view video transmission poses great challenges because of its data size and dimension. Therefore, how to design efficient 3D scene representations and coding (of luminance and geometry) has become a critical research topic. Recently, the graph-based representation (GBR) is introduced, which provides a lossless compression of multi-view geometry by connecting informative pixels among views. This representation has been shown as a promising alternative to the classical depth-based representation, where the view synthesis accuracy is hard to control. In this work, we study the luminance compression under GBR, which is not well considered in existing literature. With a proper structural reformulation, we show that the graph-based transform can be applied on the GBR paradigm, hence better extracting the correlation among pixels along graph connections. Moreover, we extend the popular SPIHT coding scheme to further improve coding efficiency. The experimental results show that our method leads to better RD coding performance as compared the classical luminance coding algorithms

Macrophage migration inhibitory factor induces vascular leakage via autophagy

Vascular leakage is an important feature of acute inflammatory shock, which currently has no effective treatment. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that can induce vascular leakage and plays an important role in the pathogenesis of shock. However, the mechanism of MIF-induced vascular leakage is still unclear. In this study, using recombinant MIF (rMIF), we demonstrated that MIF induced disorganization and degradation of junction proteins and increased the permeability of human endothelial cells in vitro. Western blotting analysis showed that rMIF treatment induced LC3 conversion and p62 degradation. Inhibition of autophagy with a PI3K inhibitor (3-MA), a ROS scavenger (NAC) or autophagosomal-lysosomal fusion inhibitors (bafilomycin A1 and chloroquine) rescued rMIF-induced vascular leakage, suggesting that autophagy mediates MIF-induced vascular leakage. The potential involvement of other signaling pathways was also studied using different inhibitors, and the results suggested that MIF-induced vascular leakage may occur through the ERK pathway. In conclusion, we showed that MIF triggered autophagic degradation of endothelial cells, resulting in vascular leakage. Inhibition of MIF-induced autophagy may provide therapeutic targets against vascular leakage in inflammatory shock

Assessment of Flood Risk Map under Climate Change RCP8.5 Scenarios in Taiwan

Climate change has exerted a significant global impact in recent years, and extreme weather-related hazards and incidents have become the new normal. For Taiwan in particular, the corresponding increase in disaster risk threatens not only the environment but also the lives, safety, and property of people. This highlights the need to develop a methodology for mapping disaster risk under climate change and delineating those regions that are potentially high-risk areas requiring adaptation to a changing climate in the future. This study provides a framework of flood risk map assessment under the RCP8.5 scenario by using different spatial scales to integrate the projection climate data of high resolution, inundation potential maps, and indicator-based approach at the end of the 21st century in Taiwan. The reference period was 1979–2003, and the future projection period was 2075–2099. High-resolution climate data developed by dynamic downscaling of the MRI-JMA-AGCM model was used to assess extreme rainfall events. The flood risk maps were constructed using two different spatial scales: the township level and the 5 km × 5 km grid. As to hazard-vulnerability(H-V) maps, users can overlay maps of their choice—such as those for land use distribution, district planning, agricultural crop distribution, or industrial distribution. Mapping flood risk under climate change can support better informed decision-making and policy-making processes in planning and preparing to intervene and control flood risks. The elderly population distribution is applied as an exposure indicator in order to guide advance preparation of evacuation plans for high-risk areas. This study found that higher risk areas are distributed mainly in northern and southern parts of Taiwan and the hazard indicators significantly increase in the northern, north-eastern, and southern regions under the RCP8.5 scenario. Moreover, the near-riparian and coastal townships of central and southern Taiwan have higher vulnerability levels. Approximately 14% of townships have a higher risk level of flooding disaster and another 3% of townships will become higher risk. For higher-risk townships, adaptation measures or strategies are suggested to prioritize improving flood preparation and protecting people and property. Such a flood risk map can be a communication tool to effectively inform decision- makers, citizens, and stakeholders about the variability of flood risk under climate change. Such maps enable decision-makers and national spatial planners to compare the relative flood risk of individual townships countrywide in order to determine and prioritize risk adaptation areas for planning spatial development policies

Assessment of Flood Risk Map under Climate Change RCP8.5 Scenarios in Taiwan

Climate change has exerted a significant global impact in recent years, and extreme weather-related hazards and incidents have become the new normal. For Taiwan in particular, the corresponding increase in disaster risk threatens not only the environment but also the lives, safety, and property of people. This highlights the need to develop a methodology for mapping disaster risk under climate change and delineating those regions that are potentially high-risk areas requiring adaptation to a changing climate in the future. This study provides a framework of flood risk map assessment under the RCP8.5 scenario by using different spatial scales to integrate the projection climate data of high resolution, inundation potential maps, and indicator-based approach at the end of the 21st century in Taiwan. The reference period was 1979–2003, and the future projection period was 2075–2099. High-resolution climate data developed by dynamic downscaling of the MRI-JMA-AGCM model was used to assess extreme rainfall events. The flood risk maps were constructed using two different spatial scales: the township level and the 5 km × 5 km grid. As to hazard-vulnerability(H-V) maps, users can overlay maps of their choice—such as those for land use distribution, district planning, agricultural crop distribution, or industrial distribution. Mapping flood risk under climate change can support better informed decision-making and policy-making processes in planning and preparing to intervene and control flood risks. The elderly population distribution is applied as an exposure indicator in order to guide advance preparation of evacuation plans for high-risk areas. This study found that higher risk areas are distributed mainly in northern and southern parts of Taiwan and the hazard indicators significantly increase in the northern, north-eastern, and southern regions under the RCP8.5 scenario. Moreover, the near-riparian and coastal townships of central and southern Taiwan have higher vulnerability levels. Approximately 14% of townships have a higher risk level of flooding disaster and another 3% of townships will become higher risk. For higher-risk townships, adaptation measures or strategies are suggested to prioritize improving flood preparation and protecting people and property. Such a flood risk map can be a communication tool to effectively inform decision- makers, citizens, and stakeholders about the variability of flood risk under climate change. Such maps enable decision-makers and national spatial planners to compare the relative flood risk of individual townships countrywide in order to determine and prioritize risk adaptation areas for planning spatial development policies

Metabolic syndrome risk in adult coffee drinkers with the rs301 variant of the LPL gene

Abstract Background Metabolic syndrome (MetS), a cluster of metabolic and cardiovascular risk factors is influenced by environmental, lifestyle, and genetic factors. We explored whether coffee consumption and the rs301 variant of the lipoprotein lipase (LPL) gene are related to MetS. Methods We conducted multiple logistic regression analyses using data gathered from 9523 subjects in Taiwan Biobank (TWB). Results Our findings indicated that individuals who consumed coffee had a reduced odds ratio (OR) for MetS (0.750 (95% confidence interval [CI] 0.653–0.861) compared to non-coffee drinkers. Additionally, the risk of MetS was lower for individuals with the ‘TC’ and ‘CC’ genotypes of rs301 compared to those with the ‘TT’ genotype. Specifically, the OR for MetS was 0.827 (95% CI 0.721–0.949) for the ‘TC’ genotype and 0.848 (95% CI 0.610–1.177) for the ‘CC’ genotype. We observed an interaction between coffee consumption and the rs301 variant, with a p-value for the interaction of 0.0437. Compared to the reference group (‘no coffee drinking/TT’), the ORs for MetS were 0.836 (95% CI 0.706–0.992) for ‘coffee drinking/TT’, 0.557 (95% CI 0.438–0.707) for ‘coffee drinking/TC’, and 0.544 (95% CI 0.319–0.927) for ‘coffee drinking/CC’. Notably, MetS was not observed in non-coffee drinkers regardless of their rs301 genotype. Conclusion Our findings suggest that rs301 genotypes may protect against MetS in Taiwanese adults who consume coffee compared to non-coffee drinkers