Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand

Canine tick-borne pathogens (CTBPs) such as Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis are important pathogens in dogs worldwide. Rhipicephalus sanguineus sensu lato, the main vector of several CTBPs, is the most common tick species found on dogs in Thailand. The present study identified CTBPs in dogs and ticks infested dogs. Samples (360 dog blood samples and 85 individual ticks) were collected from stray dogs residing in 37 temples from 24 districts in Bangkok and screened for CTBPs using molecular techniques. The most common CTBP found infecting dogs in this study was Ehrlichia canis (38.3%) followed by Mycoplasma haemocanis (34.2%), Hepatozoon canis (19.7%), Babesia vogeli (18.1%), and Anaplasma platys (13.9%), respectively. Furthermore, A. platys (22.4%) was the most common CTBP in ticks followed by M. haemocanis (18.8%), B. vogeli (9.4%), H. canis (5.9%), and E. canis (2.4%), respectively. The detection of CTBPs from the present study highlights the potential risk of infections that may occur in stray dogs and their ticks residing in Bangkok temples. These findings underline the importance of performing active surveys to understand the complexity of distributions of CTBPs in dogs and their ticks in Thailand

Detection of Giardia duodenalis Zoonotic Assemblages AI and BIV in Pet Prairie Dogs (Cynomys ludovicanus) in Bangkok, Thailand

Giardia is a flagellate protozoa that can be transmitted via direct contact and by consuming contaminated water. It is pathogenic in humans and various other animals, including exotic pets. Pet prairie dogs are popular in Thailand, but they have not been investigated regarding giardiasis. Giardia infection was measured, and genetic characterization was performed to investigate the zoonotic potential of Giardia carried by pet prairie dogs. In total, 79 fecal samples were examined from prairie dogs visiting the Kasetsart University Veterinary Teaching Hospital during 2017–2021. Simple floatation was conducted. Two Giardia-positive samples were submitted for DNA extraction, PCR targeting the Giardiassu rRNA, tpi and gdh genes was performed, and genetic characterization using sequencing analysis was conducted. Risk factors associated with Giardia infection were analyzed. Giardia infection was found in 11 out of the 79 pet prairie dogs (13.9%). Giardia infection was significantly higher in male prairie dogs (p = 0.0345). Coccidia cysts (12.7%), the eggs of nematodes (6.3%), and amoeba cysts (2.5%) were also detected. Genetic characterization of the two Giardia-positive samples revealed that they were G. duodenalis assemblage A, sub-genotypes AI and assemblage B, and sub-genotype BIV, the zoonotic assemblages. This was the first report of Giardia infection in pet prairie dogs in Bangkok, Thailand. The results revealed that these pet prairie dogs in Thailand were infected with zoonotic assemblages of G. duodenalis sub-genotype AI, which might have been derived from animal contaminants, whereas sub-genotype BIV might have been derived from human contaminants. Owners of prairie dogs might be at risk of giardiasis or be the source of infection to their exotic pets

Toxocara canis and Toxocara cati in Stray Dogs and Cats in Bangkok, Thailand: Molecular Prevalence and Risk Factors

Toxocara canis and Toxocara cati are known as common roundworm in dogs and cats, respectively. The objective of the current study was to determine the molecular prevalence and risk factors associated with T. canis and T. cati infections in stray dogs and cats, respectively, in Bangkok, Thailand. In total, 1000 fecal samples (from 500 dogs and 500 cats) were collected from stray dogs and cats residing in Bangkok temples. DNA was extracted and screened for T. canis and T. cati using polymerase chain reaction (PCR) with the internal transcribed spacer (ITS) region. The overall prevalence of T. canis in the stray dogs was 5.4% (27/500) and 0.6% (3/500) for T. cati in the stray cats. Toxocara canis infections were detected in stray dogs from 11 out of 48 districts (22.9%), with Bang Khen district having the highest proportion of infected dogs, whereas T. cati infections were only detected in the stray cats in Lat Krabang district. These results indicated that stray animals residing in Bangkok temples pose a considerable public health risk due to zoonotic parasites, especially T. canis

Prevalence of Trypanosoma evansi Infection Causing Abortion in Dairy Cows in Central Thailand

ABSTRACT Abortion in dairy cows is the major factor affecting livestock development in Thailand and is caused by many diseases. Trypanosomosis is one of these factors and also results in an immunosuppressive effect in cattle. The objective of this study was to investigate the prevalence of trypanosomosis in dairy cows in central Thailand. From March to September 2007, 544 samples were collected from 105 farms in the five major dairy provinces of Kanchanaburi, Ratchaburi, Nakhon Pathom, Saraburi and Lop Buri. ELISA was performed to test all sera. The overall prevalence of T. evansi infection in dairy cows was 8.1% (44/544) and herd prevalence was 19.2% (20/105). The highest individual prevalence was found at Saraburi (17.4%, 21/121) but the highest number of herd infections was at Nakhon Pathom (30%, 6/ 20). The parity-four and four-plus cows were 3.7 times more likely to be infected than heifers and parity-one cows (P<0.034). Large herds (40 milking cows) were found to be 5.4 times more infected than small herds (P<0.021). The results found that trypanosome infection might be the predisposing cause of other diseases and is a barrier to productivity gains in dairy herds