An efficient method for callus induction of an important medicinal plant (Sarcostemma brevistigma) from stem segments

The present study was undertaken to evaluate the most suitable concentration of growth regulators i.e., IAA, NAA, 2,4-D with BAP and Kin for callus induction. Stems were proved to be the best explant for culture, which were grown on MS basal medium with different concentration of various growth regulators. The standard plant tissue culture protocol for callus culture was adopted. The highest efficiency of callus formation was observed in the medium containing different concentration of 2, 4-D and BAP. In vitro generated callus can be used as a source for the isolation of secondary metabolites from Sarcostemma brevistigma