Aktivitas System Analyst Kegiatan Akademi Garudaku dalam Platform Garudaku

Dalam menempuh MBKM Proyek Independen, Penulis mendapatkan kesempatan untuk ikut serta sebagai penulis naskah dari film pendek dengan judul "Di Sini Jual Makanan" Kucing yang diproduksi oleh Acierto Visuals. Film pendek ini ditulis dengan harapan untuk menjadi karya yang bisa menjadi "sentilan" kepada sesama filmmaker dalam membuat sebuah film dokumenter. Penulis menjalani tahap-tahap yang dilakukan seorang penulis naskah dari development sampai poscaproduksi. Dari situ, Penulis melawati berbagai masalah dan mempelajari banyak hal dalam program MBKM Proyek Independen

The Role of School Leadership in Parental Involvment in Accelerated Christian Education Curriculum

Peran orang tua juga terbukti memiliki pengaruh signifikan terhadap perkembangan anak secara akademis di sekolah. Pada prakteknya, masih banyak orang tua yang beranggapan bahwa pendidikan anak merupakan tugas sekolah, sehingga salah satu tugas pemimpin sekolah adalah untuk meningkatkan keterlibatan orang tua di sekolah. Tujuan dari penelitian ini adalah untuk meneliti dampak keterlibatan orang tua pada sekolah XYZ yang menggunakan kurikulum A.C.E, cara pemimpin meningkatkan keterlibatan orang tua, serta tantangan-tantangannya. Metode yang digunakan dalam penelitian adalah metode penelitian kualitatif studi kasus dengan melibatkan 12 partisipan dari pemimpin sekolah, guru kelas, dan orang tua. Penelitian menemukan bahwa keterlibatan orang tua memengaruhi karakter siswa yang secara tidak langsung berhubungan dengan pencapaian akademik siswa, dan pemimpin sekolah memegang peranan penting dalam keterlibatan orang tua dengan menyediakan lingkungan yang kondusif dan secara konsisten mengomunikasikan visi pada orang tua. Tantangan dalam keterlibatan orang tua mencakup inisiatif dan kesibukan orang tua, serta keterbatasan pemimpin

Fluid flow measurement using electrical and optical fibre strain gauges.

The design, development and calibration of three flow sensors to measure the speed and direction of fluid flow is presented in this thesis. The force exerted by the fluid flow on the sensors are measured using strain gauges. Multidirectional fluid flow measurement has been made possible by vectorial addition of the orthogonal flow components. The fluid speed and direction are generated irrespective of each other. Electrical resistance strain gauges are used as the force measuring device for the first version of the flow meter. These strain gauges are bonded to the four longitudinal surfaces of a square-sectioned, elastic, rubber cantilever having a drag element attached to its free end. An attempt has been made to optimise the shape and dimensions of the elastic beam to obtain a constant drag co-efficient over a wide flow range. Calibration of the electrical strain gauge flow sensor has been performed in a wind tunnel to measure air flow. The sensor has a repeatability of 0.02%, linearity within 2% and a resolution of 0.43 m/s. The most noteworthy feature of the flow sensor is its quick response time of 50 milliseconds. The sensor is able to generate a measurement of flow direction in two dimensions with a resolution of 3.6". Preliminary measurements in a water tank enabled the speed of water to be measured with a resolution of 0.02 m/s over a range from 0 to 0.4 m/s. An optical fibre strain sensor has been designed and developed by inserting grooves into a multimode plastic optical fibre. As the fibre bends, the variation in the angle of the grooves causes an intensity modulation of the light transmitted through the fibre. A mathematical model has been developed which has been experimentally verified in the laboratory. The electrical strain gauge was replaced by the fibre optic strain gauge in the second version of the flow sensor. Two dimensional flow measurement was made possible by attaching two such optical fibre strain gauges on the adjacent sides of the square sectioned rubber beam. The optical fibre flow sensor was successfully calibrated in a wind tunnel to generate both the magnitude and direction of the velocity of air. The flow sensor had a repeatability of 0.3% and measured the wind velocity up to 30 M/s with a magnitude resolution of 1.3 m/s and a direction resolution of 5.9'. The third version of the flow sensor has used the grooved optical fibre strain sensor by itself without the rubber beam to measure the fluid flow. Wind tunnel calibration has been performed to measure two dimensional wind flow up to 35 m/s with a resolution of 0.96 m/s

Draft Genome Sequences of 15 Isolates of Listeria monocytogenes Serotype 1/2a, Subgroup ST204

Listeria monocytogenes sequence type 204 (ST204) strains have been isolated from a range of food, environmental, and clinical sources in Australia. This study describes the draft genome sequences of 15 isolates collected from meat and dairy associated sources

Comparative Genomics of the Listeria monocytogenes ST204 Subgroup

The ST204 subgroup of Listeria monocytogenes is among the most frequently isolated in Australia from a range of environmental niches. In this study we provide a comparative genomics analysis of food and food environment isolates from geographically diverse sources. Analysis of the ST204 genomes showed a highly conserved core genome with the majority of variation seen in mobile genetic elements such as plasmids, transposons and phage insertions. Most strains (13/15) harbored plasmids, which although varying in size contained highly conserved sequences. Interestingly 4 isolates contained a conserved plasmid of 91,396 bp. The strains examined were isolated over a period of 12 years and from different geographic locations suggesting plasmids are an important component of the genetic repertoire of this subgroup and may provide a range of stress tolerance mechanisms. In addition to this 4 phage insertion sites and 2 transposons were identified among isolates, including a novel transposon. These genetic elements were highly conserved across isolates that harbored them, and also contained a range of genetic markers linked to stress tolerance and virulence. The maintenance of conserved mobile genetic elements in the ST204 population suggests these elements may contribute to the diverse range of niches colonized by ST204 isolates. Environmental stress selection may contribute to maintaining these genetic features, which in turn may be co-selecting for virulence markers relevant to clinical infection with ST204 isolates

Characterisation of Listeria monocytogenes food-associated isolates to assess environmental fitness and virulence potential

The ability of Listeria monocytogenes isolates to survive within the food production environment (FPE), as well as virulence, varies greatly between strains. There are specific genetic determinants that have been identified which can strongly influence a strains ability to survive in the FPE and/or within human hosts. In this study, we assessed the FPE fitness and virulence potential, including efficacy of selected hygiene or treatment intervention, against 52 L. monocytogenes strains isolated from various food and food environment sources. Phenotypic tests were performed to determine the minimum inhibitory concentration of cadmium chloride and benzalkonium chloride and the sensitivities to five clinically relevant antibiotics. A genomic analysis was also performed to identify resistance genes correlating to the observed phenotypic resistance profiles, along with genetic determinants of interest which may elude to the FPE fitness and virulence potential. A transposon element containing a novel cadmium resistance gene, cadA7, a Tn916 variant insert in the hypervariable Listeria genomic island 1 region and an LGI2 variant were identified. Resistance to cadmium and disinfectants was prevalent among isolates in this study, although no resistance to clinically important antimicrobials was observed. Potential hypervirulent strains containing full length inlA, LIPI-1 and LIPI-3 were also identified in this study. Cumulatively, the results of this study show a vast array of FPE survival and pathogenicity potential among food production-associated isolates, which may be of concern for food processing operators and clinicians regarding L. monocytogenes strains colonising and persisting within the FPE, and subsequently contaminating food products then causing disease in at risk population groups

From DNA sequence to application: possibilities and complications

The development of sophisticated genetic tools during the past 15 years have facilitated a tremendous increase of fundamental and application-oriented knowledge of lactic acid bacteria (LAB) and their bacteriophages. This knowledge relates both to the assignments of open reading frames (ORF’s) and the function of non-coding DNA sequences. Comparison of the complete nucleotide sequences of several LAB bacteriophages has revealed that their chromosomes have a fixed, modular structure, each module having a set of genes involved in a specific phase of the bacteriophage life cycle. LAB bacteriophage genes and DNA sequences have been used for the construction of temperature-inducible gene expression systems, gene-integration systems, and bacteriophage defence systems. The function of several LAB open reading frames and transcriptional units have been identified and characterized in detail. Many of these could find practical applications, such as induced lysis of LAB to enhance cheese ripening and re-routing of carbon fluxes for the production of a specific amino acid enantiomer. More knowledge has also become available concerning the function and structure of non-coding DNA positioned at or in the vicinity of promoters. In several cases the mRNA produced from this DNA contains a transcriptional terminator-antiterminator pair, in which the antiterminator can be stabilized either by uncharged tRNA or by interaction with a regulatory protein, thus preventing formation of the terminator so that mRNA elongation can proceed. Evidence has accumulated showing that also in LAB carbon catabolite repression in LAB is mediated by specific DNA elements in the vicinity of promoters governing the transcription of catabolic operons. Although some biological barriers have yet to be solved, the vast body of scientific information presently available allows the construction of tailor-made genetically modified LAB. Today, it appears that societal constraints rather than biological hurdles impede the use of genetically modified LAB.

Population Genomics and Phylogeography of an Australian Dairy Factory Derived Lytic Bacteriophage

In this study, we present the full genomic sequences and evolutionary analyses of a serially sampled population of 28 Lactococcus lactis–infecting phage belonging to the 936-like group in Australia. Genome sizes were consistent with previously available genomes ranging in length from 30.9 to 32.1 Kbp and consisted of 55–65 open reading frames. We analyzed their genetic diversity and found that regions of high diversity are correlated with high recombination rate regions (P value = 0.01). Phylogenetic inference showed two major clades that correlate well with known host range. Using the extended Bayesian Skyline model, we found that population size has remained mostly constant through time. Moreover, the dispersion pattern of these genomes is in agreement with human-driven dispersion as suggested by phylogeographic analysis. In addition, selection analysis found evidence of positive selection on codon positions of the Receptor Binding Protein (RBP). Likewise, positively selected sites in the RBP were located within the neck and head region in the crystal structure, both known determinants of host range. Our study demonstrates the utility of phylogenetic methods applied to whole genome data collected from populations of phage for providing insights into applied microbiology

Phylogeographic analysis reveals multiple international transmission events have driven the global emergence of Escherichia coli O157:H7

This work was supported by: Scotland by Food Standards Scotland [Grant Number FS102029] and University of Aberdeen; New Zealand, Institute of Environmental Science and Research; Canada, the Public Health Agency of Canada; United States, United States Department of AgriculturePeer reviewedPostprin