26,334 research outputs found
A Proton Magnetic Resonance Study of the Association of Lysozyme with Monosaccharide Inhibitors
It has been shown that the acetamido methyl protons of N-acetyl-d-glucosamine undergo a chemical shift to higher fields in their proton magnetic resonance spectrum when the inhibitor is bound to lysozyme. The observed chemical shift in the presence of the enzyme is different for the agr- and ß-anomeric forms of 2-acetamido-2-deoxy-d-glucopyranose indicating either a difference in the affinity of the anomeric forms for lysozyme or different magnetic environments for the methyl protons in their enzyme-bound state. That the agr- and ß-anomeric forms of GlcAc bind to lysozyme in a competitive fashion was indicated by observing the proton magnetic resonance spectra in the presence of 2-acetamido-d3-2-deoxy-agr-d-glucopyranose. The methyl glycosides, methyl-agr-GlcAc and methyl-ß-GlcAc, were also shown to bind competitively with both anomers of GlcAc. Quantitative analysis of the chemical shift data observed for the association of GlcAc with lysozyme was complicated by the mutarotation of GlcAc between its agr- and ß-anomeric forms. However, in the case of the methyl glucosides, where the conformation of each anomer is frozen, it was possible to analyze the chemical shift data in a straightforward manner, and the dissociation constant as well as the chemical shift of the acetamido methyl protons of the enzyme-inhibitor complex was determined for both anomers. The results indicate that the two anomers of methyl-GlcAc bind to lysozyme with slightly different affinities but that the acetamido methyl groups of both anomers experience identical magnetic environments in the enzyme-inhibitor complex
A New Algorithm for Protein Design
We apply a new approach to the reverse protein folding problem. Our method
uses a minimization function in the design process which is different from the
energy function used for folding. For a lattice model, we show that this new
approach produces sequences that are likely to fold into desired structures.
Our method is a significant improvement over previous attempts which used the
energy function for designing sequences.Comment: 10 pages latex 2.09 no figures. Use uufiles to decod
Scalar Wave Falloff in Asymptotically Anti-de Sitter Backgrounds
Conformally invariant scalar waves in black hole spacetimes which are
asymptotically anti-de Sitter are investigated. We consider both the
-dimensional black hole and -dimensional Schwarzschild-anti-de
Sitter spacetime as backgrounds. Analytical and numerical methods show that the
waves decay exponentially in the dimensional black hole background.
However the falloff pattern of the conformal scalar waves in the
Schwarzschild-anti-de Sitter background is generally neither exponential nor an
inverse power rate, although the approximate falloff of the maximal peak is
weakly exponential. We discuss the implications of these results for mass
inflation.Comment: 34 pages, Latex, 26 figures, uses psfi
Gravitational Collapse of a Massless Scalar Field and a Perfect Fluid with Self-Similarity of the First Kind in (2+1) Dimensions
Self-similar solutions of a collapsing perfect fluid and a massless scalar
field with kinematic self-similarity of the first kind in 2+1 dimensions are
obtained. Their local and global properties of the solutions are studied. It is
found that some of them represent gravitational collapse, in which black holes
are always formed, and some may be interpreted as representing cosmological
models.Comment: 13 page
Geometrically Reduced Number of Protein Ground State Candidates
Geometrical properties of protein ground states are studied using an
algebraic approach. It is shown that independent from inter-monomer
interactions, the collection of ground state candidates for any folded protein
is unexpectedly small: For the case of a two-parameter Hydrophobic-Polar
lattice model for -mers, the number of these candidates grows only as .
Moreover, the space of the interaction parameters of the model breaks up into
well-defined domains, each corresponding to one ground state candidate, which
are separated by sharp boundaries. In addition, by exact enumeration, we show
there are some sequences which have one absolute unique native state. These
absolute ground states have perfect stability against change of inter-monomer
interaction potential.Comment: 9 page, 4 ps figures are include
CONCENTRIC TUBE-FOULING RIG FOR INVESTIGATION OF FOULING DEPOSIT FORMATION FROM PASTEURISER OF VISCOUS FOOD LIQUID
This paper reports the work on developing concentric tube-fouling rig, a new fouling deposit monitoring device. This device can detect and quantify the level of fouling deposit formation. It can also functioning as sampler for fouling deposit study, which can be attached at any food processing equipment. The design is initiated with conceptual design. The rig is designed with inner diameter of 7 cm and with tube length of 37 cm. A spiral insert with 34.5 cm length and with 5.4 cm diameter is fitted inside the tube to ensure the fluid flows around the tube. In this work, the rig is attached to the lab-scale concentric tube-pasteurizer to test its effectiveness and to collect a fouling sample after pasteurization of pink guava puree. Temperature changes are recorded during the pasteurization and the data is used to plot the heat transfer profile. Thickness of the fouling deposit is also measured. The trends for thickness, heat resistance profile and heat transfer profile for concentric tube-fouling rig matched the trends obtained from lab-scale concentric tube-pasteurizer very well. The findings from this work have shown a good potential of this rig however there is a limitation with spiral insert, which is discussed in this paper
New Charged Dilaton Solutions in 2+1 Dimensions and Solutions with Cylindrical Symmetry in 3+1 Dimensions
We report a new family of solutions to Einstein-Maxwell-dilaton gravity in
2+1 dimensions and Einstein-Maxwell gravity with cylindrical symmetry in 3+1
dimensions. A set of static charged solutions in 2+1 dimensions are obtained by
a compactification of charged solutions in 3+1 dimensions with cylindrical
symmetry. These solutions contain naked singularities for certain values of the
parameters considered. New rotating charged solutions in 2+1 dimensions and 3+1
dimensions are generated treating the static charged solutions as seed metrics
and performing transformations.Comment: Latex. No figure
Exact Black Hole and Cosmological Solutions in a Two-Dimensional Dilaton-Spectator Theory of Gravity
Exact black hole and cosmological solutions are obtained for a special
two-dimensional dilaton-spectator () theory of gravity. We show how
in this context any desired spacetime behaviour can be determined by an
appropriate choice of a dilaton potential function and a ``coupling
function'' in the action. We illustrate several black hole solutions
as examples. In particular, asymptotically flat double- and multiple- horizon
black hole solutions are obtained. One solution bears an interesting
resemblance to the string-theoretic black hole and contains the same
thermodynamic properties; another resembles the Reissner-Nordstrom
solution. We find two characteristic features of all the black hole solutions.
First the coupling constants in must be set equal to constants of
integration (typically the mass). Second, the spectator field and its
derivative both diverge at any event horizon. A test particle with
``spectator charge" ({\it i.e.} one coupled either to or ),
will therefore encounter an infinite tidal force at the horizon or an
``infinite potential barrier'' located outside the horizon respectively. We
also compute the Hawking temperature and entropy for our solutions. In
cosmology, two non-singular solutions which resemble two exact solutions
in string-motivated cosmology are obtained. In addition, we construct a
singular model which describes the standard non-inflationary big bang
cosmology (). Motivated by the
similaritiesbetween and gravitational field equations in
cosmology, we briefly discuss a special dilaton-spectator action
constructed from the bosonic part of the low energy heterotic string action andComment: 34 pgs. Plain Tex, revised version contains some clarifying comments
concerning the relationship between the constants of integration and the
coupling constants
Statistical Properties of Contact Maps
A contact map is a simple representation of the structure of proteins and
other chain-like macromolecules. This representation is quite amenable to
numerical studies of folding. We show that the number of contact maps
corresponding to the possible configurations of a polypeptide chain of N amino
acids, represented by (N-1)-step self avoiding walks on a lattice, grows
exponentially with N for all dimensions D>1. We carry out exact enumerations in
D=2 on the square and triangular lattices for walks of up to 20 steps and
investigate various statistical properties of contact maps corresponding to
such walks. We also study the exact statistics of contact maps generated by
walks on a ladder.Comment: Latex file, 15 pages, 12 eps figures. To appear on Phys. Rev.
Photonic Clusters
We show through rigorous calculations that dielectric microspheres can be
organized by an incident electromagnetic plane wave into stable cluster
configurations, which we call photonic molecules. The long-range optical
binding force arises from multiple scattering between the spheres. A photonic
molecule can exhibit a multiplicity of distinct geometries, including
quasicrystal-like configurations, with exotic dynamics. Linear stability
analysis and dynamical simulations show that the equilibrium configurations can
correspond with either stable or a type of quasi-stable states exhibiting
periodic particle motion in the presence of frictional dissipation.Comment: 4 pages, 3 figure
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