Inspiration strikes: Partnering with experienced student consultants to prepare to partner with new student consultants

This article examines the process of partnering with experienced undergraduate SaLT consultants to ready educators to work in partnership with high school student consultants.  We discuss the benefits of preparation: giving time to building relationships, being ready to receive feedback, learning to trust the process of student partnership, and honoring authenticity and vulnerability

Quantifying signal changes in nano-wire based biosensors

In this work, we present a computational methodology for predicting the change in signal (conductance sensitivity) of a nano-BioFET sensor (a sensor based on a biomolecule binding another biomolecule attached to a nano-wire field effect transistor) upon binding its target molecule. The methodology is a combination of the screening model of surface charge sensors in liquids developed by Brandbyge and co-workers [Sørensen et al., Appl. Phys. Lett., 2007, 91, 102105], with the PROPKA method for predicting the pH-dependent charge of proteins and protein-ligand complexes, developed by Jensen and co-workers [Li et al., Proteins: Struct., Funct., Bioinf., 2005, 61, 704-721, Bas et al., Proteins: Struct., Funct., Bioinf., 2008, 73, 765-783]. The predicted change in conductance sensitivity based on this methodology is compared to previously published data on nano-BioFET sensors obtained by other groups. In addition, the conductance sensitivity dependence from various parameters is explored for a standard wire, representative of a typical experimental setup. In general, the experimental data can be reproduced with sufficient accuracy to help interpret them. The method has the potential for even more quantitative predictions when key experimental parameters (such as the charge carrier density of the nano-wire or receptor density on the device surface) can be determined (and reported) more accurately. © 2011 The Royal Society of Chemistry

Constitution as Promise

Constitution as Promise contends that constitutions are sets of promises. As such, it argues that they must be interpreted in a living constitutionalist manner. Chapter One argues that constitutions meet the analytic criteria to be considered promises. It is argued that constitutions are expressions of the intention of a government to bind itself to a set of principles. Absent this expression, citizens lack assurance of the protection of their rights and legal recourse when their rights are violated. Chapter Two considers the use of promise in contract theory and investigates its viability in constitutional theory. Some theories of contract are skeptical of promise as a basis for contract. The chapter argues that while promise may be an inadequate moral underpinning for the law of contract, it is apt for the law of constitutions. Chapter Three notes that constitutions are sets of vague promises. Vague promises ought not be interpreted solely in accordance with the intentions of promisors or promisees. Traditional forms of originalism contend that constitutions should be interpreted according to the intentions of their framers. So, constitution as promise rules out traditional forms of originalism. Chapter Four considers the positive consequences of constitution as promise. It argues that vague promises ought to be interpreted through a negotiation process between promisor and promisee. This negotiation should consider what moral reasoning reveals about the promise’s terms, the context in which the promise was uttered, the capacities and competing obligations of the promisor, and the expectations of the promisee. To properly consider these factors, the chapter maintains that the negotiation must occur on a case-by-case basis, incrementally specifying the promise’s terms. The chapter then notes the similarities between this negotiation process and the interpretive suggestions of living constitutionalism. It concludes that living constitutionalism is entailed by the promissory nature of constitutions.ThesisMaster of Philosophy (MA)Constitution as Promise investigates the interpretive consequences of conceptualizing constitutions as promises from governments to citizens. It first argues that constitutions satisfy the criteria to be considered promises. It then maintains that the morality of promising is apt for application to constitutional law. In the third and fourth chapters, it considers how one ought to interpret vague promises. Vague promises, it argues, should be interpreted incrementally, on a case-by-case basis. The promisor and the promisee must come to an agreement about what their vague promise requires, as new cases arise. When they cannot agree, promisor and promisee need an adjudicator. Since constitutions are sets of vague promises, they must also be interpreted incrementally, on a case-by-case basis and require adjudication where agreement is impossible. Constitution as Promise concludes that the only available interpretive theory that is sensitive to constitution’s nature as vague promise is living constitutionalism. As such, constitutions ought to be interpreted in a living constitutionalist manner

Chemical Linkage to Injected Tissues Is a Distinctive Property of Oxidized Avidin

We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups. We also showed, in a mouse pre-clinical model, the usefulness of AvidinOX for the delivery of radiolabeled biotin to inoperable tumors. Taking into account that AvidinOX is the first oxidized glycoprotein known to chemically link to injected tissues, we tested in the mouse a panel of additional oxidized glycoproteins, with the aim of investigating the phenomenon. We produced oxidized ovalbumin and mannosylated streptavidin which share with avidin glycosylation pattern and tetrameric structure, respectively and found that neither of them linked significantly to cells in vitro nor to injected tissues in vivo, despite the presence of functional aldehyde groups. The study, extended to additional oxidized glycoproteins, showed that the in vivo chemical conjugation is a distinctive property of the oxidized avidin. Relevance of the high cationic charge of avidin into the stable linkage of AvidinOX to tissues is demonstrated as the oxidized acetylated avidin lost the property. Plasmon resonance on matrix proteins and cellular impedance analyses showed in vitro that avidin exhibits a peculiar interaction with proteins and cells that allows the formation of highly stable Schiff's bases, after oxidation

The weaponization of web archives: Data craft and COVID-19 publics

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The weaponization of web archives: Data craft and COVID-19 publics

An unprecedented volume of harmful health misinformation linked to the coronavirus pandemic has led to the appearance of misinformation tactics that leverage web archives in order to evade content moderation on social media platforms. Here we present newly identified manipulation techniques designed to maximize the value, longevity, and spread of harmful and non-factual content across social media using provenance information from web archives and social media analytics. After identifying conspiracy content that has been archived by human actors with the Wayback Machine, we report on user patterns of “screensampling,” where images of archived misinformation are spread via social platforms. We argue that archived web resources from the Internet Archive’s Wayback Machine and subsequent screenshots contribute to the COVID-19 “misinfodemic” in platforms. Understanding these manipulation tactics that use sources from web archives reveals something vexing about information practices during pandemics—the desire to access reliable information even after it has been moderated and fact-checked, for some individuals, will give health misinformation and conspiracy theories more traction because it has been labeled as specious content by platforms.</jats:p