Signaling Events During Induction of Plasminogen Activator Inhibitor-1 Expression by Sphingosylphosphorylcholine in Cultured Human Dermal Fibroblasts

Sphingosylphosphorylcholine (SPC) is a bioactive sphingolipid metabolite that can enhance wound healing. In a search for effectors downstream of SPC in the wound-healing process, we found that the expression of the gene for plasminogen activator inhibitor-1 (PAI-1) was significantly affected. ELISA and western blot analyses showed that SPC markedly induced PAI-1 production in human dermal fibroblasts cultured in vitro. Inhibition by pre-treatment with pertussis toxin (PTx), but not by tyrphostin A47 (a receptor tyrosine kinase inhibitor), indicated that PTx-sensitive G proteins were involved in SPC-induced PAI-1 expression. SPC elicited a rapid and transient increase in intracellular calcium levels ([Ca2+]i), measured using laser scanning confocal microscopy, which was partly mediated through PTx-sensitive G proteins. Pre-treatment with thapsigargin, but not with EGTA, abolished SPC-induced PAI-1 expression, indicating the importance of Ca2+ release from internal stores. Phorbol-12-myristate-13-acetate (PMA) induced the expression of PAI-1, and pre-treatment with Ro 31-8220 (a PKC inhibitor) markedly suppressed SPC-induced PAI-1 expression. SPC-induced PAI-1 expression was also significantly suppressed by PD98059 (a specific MAPK kinase 1/2 inhibitor). Consistent with this result, SPC stimulated the phosphorylation of p42/44 extracellular signal-regulated kinase (ERK). Together, these results suggest that SPC induces PAI-1 production through a G protein-coupled calcium increase and downstream kinase signaling events in cultured human dermal fibroblasts

Detection of Opposite Magnetic Polarity in a Light Bridge: Its Emergence and Cancellation in association with LB Fan-shaped Jets

Light bridges (LBs) are relatively bright structures that divide sunspot umbrae into two or more parts. Chromospheric LBs are known to be associated with various activities including fan-shaped jet-like ejections and brightenings. Although magnetic reconnection is frequently suggested to be responsible for such activities, not many studies present firm evidence to support the scenario. We carry out magnetic field measurements and imaging spectroscopy of a LB where fan-shaped jet-like ejections occur with co-spatial brightenings at their footpoints. We study LB fine structure and magnetic field changes using TiO images, Near-InfraRed Imaging Spectropolarimeter, and Halpha data taken by the 1.6~m Goode Solar Telescope. We detect magnetic flux emergence in the LB that is of opposite polarity to that of the sunspot. The new magnetic flux cancels with the pre-existing flux at a rate of 5.6x10^18 Mx/hr. Both the recurrent jet-like ejections and their base brightenings are initiated at the vicinity of the magnetic flux cancellation, and show apparent horizontal extension along the LB at a projected speed of up to 18.4km/s to form a fan-shaped appearance. Based on these observations, we suggest that the fan-shaped ejections may have resulted from slipping reconnection between the new flux emerging in the LB and the ambient sunspot field.Comment: 24pages, 11figures, accepted by the Ap