Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum

We evaluated the bactericidal activity of Bdellovibrio bacteriovorus, strain HD100, within blood sera against bacterial strains commonly associated with bacteremic infections, including E. coli, Klebsiella pneumoniae and Salmonella enterica. Tests show that B. bacteriovorus HD100 is not susceptible to serum complement or its bactericidal activity. After a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the B. bacteriovorus HD100 population showed a loss of only 33%. Dot blot analyses showed that this is not due to the absence of antibodies against this predator. Predation in human serum was inhibited, though, by both the osmolality and serum albumin. The activity of B. bacteriovorus HD100 showed a sharp transition between 200 and 250 mOsm/kg, and was progressively reduced as the osmolality increased. Serum albumin also acted to inhibit predation by binding to and coating the predatory cells. This was confirmed via dot blot analyses and confocal microscopy. The results from both the osmolality and serum albumin tests were incorporated into a numerical model describing bacterial predation of pathogens. In conclusion, both of these factors inhibit predation and, as such, they limit its effectiveness against pathogenic prey located within sera

Deep sea tests of a prototype of the KM3NeT digital optical module

The first prototype of a photo-detection unit of the future KM3NeT neutrino telescope has been deployed in the deepwaters of the Mediterranean Sea. This digital optical module has a novel design with a very large photocathode area segmented by the use of 31 three inch photomultiplier tubes. It has been integrated in the ANTARES detector for in-situ testing and validation. This paper reports on the first months of data taking and rate measurements. The analysis results highlight the capabilities of the new module design in terms of background suppression and signal recognition. The directionality of the optical module enables the recognition of multiple Cherenkov photons from the same (40)Kdecay and the localisation of bioluminescent activity in the neighbourhood. The single unit can cleanly identify atmospheric muons and provide sensitivity to the muon arrival directions

The Large Observatory for x-ray timing

The Large Observatory For x-ray Timing (LOFT) was studied within ESA M3 Cosmic Vision framework and participated in the final down-selection for a launch slot in 2022-2024. Thanks to the unprecedented combination of effective area and spectral resolution of its main instrument, LOFT will study the behaviour of matter under extreme conditions, such as the strong gravitational field in the innermost regions of accretion flows close to black holes and neutron stars, and the supra-nuclear densities in the interior of neutron stars. The science payload is based on a Large Area Detector (LAD, 10 m2 effective area, 2-30 keV, 240 eV spectral resolution, 1° collimated field of view) and a WideField Monitor (WFM, 2-50 keV, 4 steradian field of view, 1 arcmin source location accuracy, 300 eV spectral resolution). The WFM is equipped with an on-board system for bright events (e.g. GRB) localization. The trigger time and position of these events are broadcast to the ground within 30 s from discovery. In this paper we present the status of the mission at the end of its Phase A study

The LOFT mission concept: a status update

The Large Observatory For x-ray Timing (LOFT) is a mission concept which was proposed to ESA as M3 and M4 candidate in the framework of the Cosmic Vision 2015-2025 program. Thanks to the unprecedented combination of effective area and spectral resolution of its main instrument and the uniquely large field of view of its wide field monitor, LOFT will be able to study the behaviour of matter in extreme conditions such as the strong gravitational field in the innermost regions close to black holes and neutron stars and the supra-nuclear densities in the interiors of neutron stars. The science payload is based on a Large Area Detector (LAD, >8m2 effective area, 2-30 keV, 240 eV spectral resolution, 1 degree collimated field of view) and a Wide Field Monitor (WFM, 2-50 keV, 4 steradian field of view, 1 arcmin source location accuracy, 300 eV spectral resolution). The WFM is equipped with an on-board system for bright events (e.g., GRB) localization. The trigger time and position of these events are broadcast to the ground within 30 s from discovery. In this paper we present the current technical and programmatic status of the mission

\ensuremath{\gamma}-ray spectroscopy of low-lying yrast and non-yrast states in neutron-rich 94,95,96Kr^{94,95,96}\mathrm{Kr}

International audienceWe report on γ-ray spectroscopy of low-lying excited states in the neutron-rich Kr94,95,96 isotopes measured as part of the “Shell Evolution And Search for Two-plus energies At RIBF” (SEASTAR) campaign at the RIKEN Radioactive Isotope Beam Factory. Excited yrast and non-yrast states were observed, and half-lives extracted via geant4 simulations. In Kr94,96 candidates for the 31− state were identified. For Kr95, the prompt SEASTAR data were combined with delayed spectroscopic data measured with the EURICA array to observe transitions on top of the known (7/2)+ isomer at a level energy of 195.5(3) keV. The comparison of the new experimental results with five-dimensional collective Hamiltonian (5DCH) and mapped interacting boson model (IBM) calculations, both using the Gogny D1M interaction, could suggest oblate-prolate shape coexistence already in Kr96

Signatures of triaxiality in low-spin spectra of ⁸⁶Ge

Low-spin states of neutron-rich ⁸⁴,⁸⁶,⁸⁸Ge were measured by in-flight γ-ray spectroscopy at 270 MeV/u at the RIKEN-RIBF facility. The exotic beams have been produced by primary ²³⁸U in-flight fission reactions and impinged on the MINOS device. MINOS combines a 10-cm long LH₂ target with a Time Projection Chamber (TPC) to reconstruct the reaction vertices. The reactions were selected by the BigRIPS and the ZeroDegree spectrometers for the incoming and outgoing channels, respectively. Emitted γ radiation was detected by the NaI-array DALI2. De-excitations from the 6₁⁺, 4₁,₂⁺ , and 2₁,₂⁺ states of ⁸⁴,⁸⁶Ge and 4₁⁺ and 2₁,₂⁺ states of ⁸⁸Ge were observed. The data are compared to state-of-the-art shell model and beyond-mean-field calculations. Furthermore, a candidate for a 3₁⁺ state of ⁸⁶Ge was identified. This state plays a key role in the discussion of ground-state triaxiality of ⁸⁶Ge, along with other features of the low-energy level scheme. This work was published in [1]

Spectroscopy of neutron-rich scandium isotopes

Within the SEASTAR III campaign at the Radioactive Isotope Beam Factory, at the RIKEN Nishina Center, neutron-rich isotopes in the vicinity of ⁵³K were produced from the fragmentation of the primary ⁷⁰Zn beam on a ⁹Be target. After nucleon knockout reactions on the secondary liquid hydrogen MINOS target the known γ rays of the neutron-rich ⁵⁵Sc isotope were observed (shown in this proceedings) and γ rays from ⁵⁷,⁵⁹Sc isotopes have been identified for the first time. The evolution of the occupied nucleon orbitals of these nuclei in the ground and excited state is investigated under the prism of the tensor force

On the interaction between protein L and immunoglobulins of various mammalian species

Protein L, a cell wall molecule of certain strains of the anaerobic bacterial species Peptostreptococcus magnus, shows high affinity for human immunoglobulin (Ig) light chains. In the present study protein L was tested against a panel of human myeloma proteins of the IgG, IgM, IgA and IgE classes, and strong binding was seen with antibodies carrying kappa light chains. A high degree of specificity for Ig was demonstrated in binding experiments with human plasma proteins. Apart from human Ig, strong protein L-binding activity was also detected in the serum of 12 out of 23 tested additional mammalian species, including other primates and rodents. Subsequent analysis with purified Ig samples demonstrated the binding of protein L to Ig of important laboratory animal species such as the mouse, the rat and the rabbit. The affinity constants for the interactions between protein L and polyclonal IgG of these species were 2.6 x 10(9), 3.9 x 10(8) and 7.4 x 10(7), respectively. In non-human species, the binding of protein L was also found to be mediated through Ig light chains, and the results demonstrate the potential value of protein L as an immunochemical tool