Results of a novel screening tool measuring dietary sodium knowledge in patients with chronic kidney disease.

BackgroundReducing dietary sodium has potential to benefit patients with chronic kidney disease (CKD). Little research is available defining dietary sodium knowledge gaps in patients with pre-dialysis CKD. We designed a brief screening tool to rapidly identify patient knowledge gaps related to dietary sodium for patients with CKD not yet on dialysis.MethodsA Short Sodium Knowledge Survey (SSKS) was developed and administered to patients with pre-dialysis CKD. We also asked patients if they received counseling on dietary sodium reduction and about recommended intake limits. We performed logistic regression to examine the association between sodium knowledge and patient characteristics. Characteristics of patients who answered all SSKS questions correctly were compared to those who did not.ResultsOne-hundred fifty-five patients were surveyed. The mean (SD) age was 56.6 (15.1) years, 84 (54%) were men, and 119 (77%) were white. Sixty-seven patients (43.2%) correctly identified their daily intake sodium limit. Fifty-eight (37.4%) were unable to answer all survey questions correctly. In analysis adjusted for age, sex, race, education, health literacy, CKD stage, self-reported hypertension and attendance in a kidney education class, women and patients of non-white race had lower odds of correctly answering survey questions (0.36 [0.16,0.81]; p = 0.01 women versus men and 0.33 [0.14,0.76]; p = 0.01 non-white versus white, respectively).ConclusionsOur survey provides a mechanism to quickly identify dietary sodium knowledge gaps in patients with CKD. Women and patients of non-white race may have knowledge barriers impeding adherence to sodium reduction advice

Frequentist Analysis of the Parameter Space of Minimal Supergravity

We make a frequentist analysis of the parameter space of minimal supergravity (mSUGRA), in which, as well as the gaugino and scalar soft supersymmetry-breaking parameters being universal, there is a specific relation between the trilinear, bilinear and scalar supersymmetry-breaking parameters, A_0 = B_0 + m_0, and the gravitino mass is fixed by m_{3/2} = m_0. We also consider a more general model, in which the gravitino mass constraint is relaxed (the VCMSSM). We combine in the global likelihood function the experimental constraints from low-energy electroweak precision data, the anomalous magnetic moment of the muon, the lightest Higgs boson mass M_h, B physics and the astrophysical cold dark matter density, assuming that the lightest supersymmetric particle (LSP) is a neutralino. In the VCMSSM, we find a preference for values of m_{1/2} and m_0 similar to those found previously in frequentist analyses of the constrained MSSM (CMSSM) and a model with common non-universal Higgs masses (NUHM1). On the other hand, in mSUGRA we find two preferred regions: one with larger values of both m_{1/2} and m_0 than in the VCMSSM, and one with large m_0 but small m_{1/2}. We compare the probabilities of the frequentist fits in mSUGRA, the VCMSSM, the CMSSM and the NUHM1: the probability that mSUGRA is consistent with the present data is significantly less than in the other models. We also discuss the mSUGRA and VCMSSM predictions for sparticle masses and other observables, identifying potential signatures at the LHC and elsewhere.Comment: 18 pages 27 figure

The motivation to be sedentary predicts weight change when sedentary behaviors are reduced

<p>Abstract</p> <p>Background</p> <p>Obesity is correlated with a sedentary lifestyle, and the motivation to be active or sedentary is correlated with obesity. The present study tests the hypothesis that the motivation to be active or sedentary is correlated with weight change when children reduce their sedentary behavior.</p> <p>Methods</p> <p>The motivation to be active or sedentary, changes in weight, and accelerometer assessed physical activity were collected for 55 families with overweight/obese children who participated in a nine-week field study to examine behavior and weight change as a function of reducing sedentary behavior. Children were studied in three 3-week phases, baseline, reduce targeted sedentary behaviors by 25% and reduce targeted sedentary behaviors by 50%. The targeted sedentary behaviors included television, video game playing, video watching, and computer use.</p> <p>Results</p> <p>The reinforcing value of sedentary behavior but not physical activity, was correlated with weight change, as losing weight was associated with lower reinforcing value of sedentary behaviors. Reducing sedentary behavior was not associated with a significant change in objectively measured physical activity, suggesting the main way in which reducing sedentary behavior influenced weight change is by complementary changes in energy intake. Estimated energy intake supported the hypothesis that reducing sedentary behaviors influences weight by reducing energy intake.</p> <p>Conclusions</p> <p>These data show that the motivation to be sedentary limits the effects of reducing sedentary behavior on weight change in obese children.</p> <p>Trial registration</p> <p>ClinicalTrials.gov: <a href="http://www.clinicaltrials.gov/ct2/show/NCT00962247">NCT00962247</a></p

A conserved circadian function for the Neurofibromatosis 1 gene

Summary: Loss of the Neurofibromatosis 1 (Nf1) protein, neurofibromin, in Drosophila disrupts circadian rhythms of locomotor activity without impairing central clock function, suggesting effects downstream of the clock. However, the relevant cellular mechanisms are not known. Leveraging the discovery of output circuits for locomotor rhythms, we dissected cellular actions of neurofibromin in recently identified substrates. Herein, we show that neurofibromin affects the levels and cycling of calcium in multiple circadian peptidergic neurons. A prominent site of action is the pars intercerebralis (PI), the fly equivalent of the hypothalamus, with cell-autonomous effects of Nf1 in PI cells that secrete DH44. Nf1 interacts genetically with peptide signaling to affect circadian behavior. We extended these studies to mammals to demonstrate that mouse astrocytes exhibit a 24-hr rhythm of calcium levels, which is also attenuated by lack of neurofibromin. These findings establish a conserved role for neurofibromin in intracellular signaling rhythms within the nervous system. : Bai et al. show that the gene mutated in the disease Neurofibromatosis 1 is required for maintaining levels or cycling of calcium in circadian neurons in Drosophila and in mammalian cells. These effects likely account for effects of Nf1 on circadian behavior in Drosophila and may be relevant in explaining sleep phenotypes in patients. Keywords: circadian rhythms, neurofibromatosis 1, Drosophila, peptide signaling, cycling of calcium, mouse astrocyte

The NUHM2 after LHC Run 1

We make a frequentist analysis of the parameter space of the NUHM2, in which the soft supersymmetry (SUSY)-breaking contributions to the masses of the two Higgs multiplets, $m^2_{H_{u,d}}$, vary independently from the universal soft SUSY-breaking contributions $m^2_0$ to the masses of squarks and sleptons. Our analysis uses the MultiNest sampling algorithm with over $4 \times 10^8$ points to sample the NUHM2 parameter space. It includes the ATLAS and CMS Higgs mass measurements as well as their searches for supersymmetric jets + MET signals using the full LHC Run~1 data, the measurements of $B_s \to \mu^+ \mu^-$ by LHCb and CMS together with other B-physics observables, electroweak precision observables and the XENON100 and LUX searches for spin-independent dark matter scattering. We find that the preferred regions of the NUHM2 parameter space have negative SUSY-breaking scalar masses squared for squarks and sleptons, $m_0^2 < 0$, as well as $m^2_{H_u} < m^2_{H_d} < 0$. The tension present in the CMSSM and NUHM1 between the supersymmetric interpretation of $g_\mu - 2$ and the absence to date of SUSY at the LHC is not significantly alleviated in the NUHM2. We find that the minimum $\chi^2 = 32.5$ with 21 degrees of freedom (dof) in the NUHM2, to be compared with $\chi^2/{\rm dof} = 35.0/23$ in the CMSSM, and $\chi^2/{\rm dof} = 32.7/22$ in the NUHM1. We find that the one-dimensional likelihood functions for sparticle masses and other observables are similar to those found previously in the CMSSM and NUHM1.Comment: 20 pages latex, 13 figure

Supersymmetric Dark Matter after LHC Run 1

Different mechanisms operate in various regions of the MSSM parameter space to bring the relic density of the lightest neutralino, neutralino_1, assumed here to be the LSP and thus the Dark Matter (DM) particle, into the range allowed by astrophysics and cosmology. These mechanisms include coannihilation with some nearly-degenerate next-to-lightest supersymmetric particle (NLSP) such as the lighter stau (stau_1), stop (stop_1) or chargino (chargino_1), resonant annihilation via direct-channel heavy Higgs bosons H/A, the light Higgs boson h or the Z boson, and enhanced annihilation via a larger Higgsino component of the LSP in the focus-point region. These mechanisms typically select lower-dimensional subspaces in MSSM scenarios such as the CMSSM, NUHM1, NUHM2 and pMSSM10. We analyze how future LHC and direct DM searches can complement each other in the exploration of the different DM mechanisms within these scenarios. We find that the stau_1 coannihilation regions of the CMSSM, NUHM1, NUHM2 can largely be explored at the LHC via searches for missing E_T events and long-lived charged particles, whereas their H/A funnel, focus-point and chargino_1 coannihilation regions can largely be explored by the LZ and Darwin DM direct detection experiments. We find that the dominant DM mechanism in our pMSSM10 analysis is chargino_1 coannihilation: {parts of its parameter space can be explored by the LHC, and a larger portion by future direct DM searches.Comment: 21 pages, 8 figure

The pMSSM10 after LHC Run 1

We present a frequentist analysis of the parameter space of the pMSSM10, in which the following 10 soft SUSY-breaking parameters are specified independently at the mean scalar top mass scale Msusy = Sqrt[M_stop1 M_stop2]: the gaugino masses M_{1,2,3}, the 1st-and 2nd-generation squark masses M_squ1 = M_squ2, the third-generation squark mass M_squ3, a common slepton mass M_slep and a common trilinear mixing parameter A, the Higgs mixing parameter mu, the pseudoscalar Higgs mass M_A and tan beta. We use the MultiNest sampling algorithm with 1.2 x 10^9 points to sample the pMSSM10 parameter space. A dedicated study shows that the sensitivities to strongly-interacting SUSY masses of ATLAS and CMS searches for jets, leptons + MET signals depend only weakly on many of the other pMSSM10 parameters. With the aid of the Atom and Scorpion codes, we also implement the LHC searches for EW-interacting sparticles and light stops, so as to confront the pMSSM10 parameter space with all relevant SUSY searches. In addition, our analysis includes Higgs mass and rate measurements using the HiggsSignals code, SUSY Higgs exclusion bounds, the measurements B-physics observables, EW precision observables, the CDM density and searches for spin-independent DM scattering. We show that the pMSSM10 is able to provide a SUSY interpretation of (g-2)_mu, unlike the CMSSM, NUHM1 and NUHM2. As a result, we find (omitting Higgs rates) that the minimum chi^2/dof = 20.5/18 in the pMSSM10, corresponding to a chi^2 probability of 30.8 %, to be compared with chi^2/dof = 32.8/24 (31.1/23) (30.3/22) in the CMSSM (NUHM1) (NUHM2). We display 1-dimensional likelihood functions for SUSY masses, and show that they may be significantly lighter in the pMSSM10 than in the CMSSM, NUHM1 and NUHM2. We discuss the discovery potential of future LHC runs, e+e- colliders and direct detection experiments.Comment: 47 pages, 29 figure

Genetic Subdivision of Chemosynthetic Endosymbionts of Solemya velum along the Southern New England Coast

Population-level genetic diversity in the obligate symbiosis between the bivalve Solemya velum and its thioautotrophic bacterial endosymbiont was examined. Distinct populations along the New England coast shared a single mitochondrial genotype but were fixed for unique symbiont genotypes, indicating high levels of symbiont genetic structuring and potential symbiont-host decoupling.Organismic and Evolutionary Biolog

Whole Genome Sequencing of Rhodotorula Mucilaginosa Isolated from the Chewing Stick (Distemonanthus benthamianus): insights into Rhodotorula Phylogeny, Mitogenome Dynamics and Carotenoid Biosynthesis

In industry, the yeast Rhodotorula mucilaginosa is commonly used for the production of carotenoids. The production of carotenoids is important because they are used as natural colorants in food and some carotenoids are precursors of retinol (vitamin A). However, the identification and molecular characterization of the carotenoid pathway/s in species belonging to the genus Rhodotorula is scarce due to the lack of genomic information thus potentially impeding effective metabolic engineering of these yeast strains for improved carotenoid production. In this study, we report the isolation, identification, characterization and the whole nuclear genome and mitogenome sequence of the endophyte R. mucilaginosa RIT389 isolated from Distemonanthus benthamianus, a plant known for its anti-fungal and antibacterial properties and commonly used as chewing sticks. The assembled genome of R. mucilaginosa RIT389 is 19 Mbp in length with an estimated genomic heterozygosity of 9.29%. Whole genome phylogeny supports the species designation of strain RIT389 within the genus in addition to supporting the monophyly of the currently sequenced Rhodotorula species. Further, we report for the first time, the recovery of the complete mitochondrial genome of R. mucilaginosa using the genome skimming approach. The assembled mitogenome is at least 7,000 bases larger than that of Rhodotorula taiwanensiswhich is largely attributed to the presence of large intronic regions containing open reading frames coding for homing endonuclease from the LAGLIDADG and GIY-YIG families. Furthermore, genomic regions containing the key genes for carotenoid production were identified in R. mucilaginosa RIT389, revealing differences in gene synteny that may play a role in the regulation of the biotechnologically important carotenoid synthesis pathways in yeasts