Positional match demands of professional rugby league competition

The purpose of this study was to examine the differences in physical performance and game-specific skill demands between 5 positional groups in a professional rugby league team. Positional groups consisted of the backs (n = 8), forwards (n = 8), fullback (n = 7), hooker (n = 8), and service players (n = 8). Time-motion analysis was used to determine physical performance measures (exercise intensity, distance travelled, time, frequency, and speed measures) and game-specific skill measures (ball carries, supports, ball touches, play the balls, and tackling indices) per minute of playing time. The main finding was that the fullback completed more very high-intensity running (VHIR) because of more support runs when compared to all other positional groups (p = 0.017). THe VHIR (p = 0.004) and sprinting indices (p < 0.002) were also greater in the second half of a match for the fullback than in any other positional group. The hooker spent more time jogging than the backs and forwards (p < 0.001) and touched the ball on more occasions than any other positional group (p < 0.001). The backs spent more time walking than the forwards, hooker, and service players (p < 0.001). The forwards, hooker, and service players completed more tackles per minute during a match than the backs and fullback (p < 0.001). The fullback and forwards also ran the ball on more occasions than the backs, hooker, and service players did (p < 0.001). These results show that positional roles play an important part in determining the amount of physical and game-specific skill involvement during match play. © 2011 National Strength and Conditioning Association

Off-target effects of bacillus Calmette–Guérin vaccination on immune responses to SARS-CoV-2: implications for protection against severe COVID-19

Background and objectives: Because of its beneficial off-target effects against non-mycobacterial infectious diseases, bacillus Calmette–Guérin (BCG) vaccination might be an accessible early intervention to boost protection against novel pathogens. Multiple epidemiological studies and randomised controlled trials (RCTs) are investigating the protective effect of BCG against coronavirus disease 2019 (COVID-19). Using samples from participants in a placebo-controlled RCT aiming to determine whether BCG vaccination reduces the incidence and severity of COVID-19, we investigated the immunomodulatory effects of BCG on in vitro immune responses to SARS-CoV-2. Methods: This study used peripheral blood taken from participants in the multicentre RCT and BCG vaccination to reduce the impact of COVID-19 on healthcare workers (BRACE trial). The whole blood taken from BRACE trial participants was stimulated with γ-irradiated SARS-CoV-2-infected or mock-infected Vero cell supernatant. Cytokine responses were measured by multiplex cytokine analysis, and single-cell immunophenotyping was made by flow cytometry. Results: BCG vaccination, but not placebo vaccination, reduced SARS-CoV-2-induced secretion of cytokines known to be associated with severe COVID-19, including IL-6, TNF-α and IL-10. In addition, BCG vaccination promoted an effector memory phenotype in both CD4+ and CD8+ T cells, and an activation of eosinophils in response to SARS-CoV-2. Conclusions: The immunomodulatory signature of BCG’s off-target effects on SARS-CoV-2 is consistent with a protective immune response against severe COVID-19

Inorganic Mass Spectrometry

To establish a method for sensitive, accurate, and precise determination of Se in real samples, isotope dilution analysis using high-power nitrogen microwave-induced plasma mass spectrometry (N 2 MIP-IDMS) was conducted. In this study, freeze-dried human blood serum (Standard Reference Material, NIES No. 4) provided by NIES (National Institute for Environmental Studies) was used as a real sample. The measured isotopes of Se were 78 Se and 80 Se which are the major isotopes of Se. The appropriate amount of a Se spike solution was theoretically calculated by using an error multiplication factor (F) and was confirmed experimentally for the isotope dilution analysis. The mass discrimination effect was corrected for by using a standard Se solution for the measurement of Se isotope ratios in the spiked sample. However, the sensitivity for the detection of Se was not so good and the precision of the determination was not improved (2-3%) by N 2 MIP-IDMS with use of the conventional nebulizer. Therefore, a hydride generation system was connected to N 2 MIP-IDMS as a sample introduction system (HG-N 2 MIP-IDMS) in order to establish a more sensitive detection and a more precise determination of Se. A detection limit (3σ) of 10 pg mL -1 could be achieved, and the RSD was less than 1% at the concentration level of 5.0-10.0 ng mL -1 by HG-N 2 MIP-IDMS. The analytical results were found to be in a good agreement with those obtained by the standard addition method using conventional Ar ICPMS. It is well-known that Se is an essential element for all mammals. Se deficiency leads to deficiency syndromes, for example, Keshan disease, which is known for cardiac insufficiency that occurred in children and pregnant women in China. Problems also occur if the concentration of Se is too high; for example, gastroenteric disorders, dermatitis, and neurotic disorders are caused by excessive intake of Se. Moreover, it is well-known that the range of permissive intake amounts of Se is very narrow for human beings. Therefore, it is restricted as a toxic element in environmental standards. There are several sources of environmental Se pollution: the processes of Se refinement and the production processes of Se-containing products. For these reasons, the accurate and precise determination of trace levels of Se in environmental and biological samples is required, and studies of Se determination have been reported by several groups. [1][2][3][4][5][6][7][8][9][10][11] Because Ar ICPMS can measure multiple elements at a concentration range from ng mL -1 to fg mL -1 , it has widespread use in the determination of trace elements in various samples. 12-25 However

A comparison of match demands between elite and semi-elite rugby league competition

The purpose of this study was to examine differences in physical performance and game-specific skills between elite and semi-elite rugby league players during match-play. Time-motion analysis was used to determine physical and game-specific skill match demands in 17 elite and 22 semi-elite rugby league players. Physical performance (time, intensity of exercise, frequency, repeated-sprint ability, and speed measures) and game-specific skill measures (ball carries, supports, ball touches, play-the-balls, and tackles) were recorded per minute of playing time. The main findings were that total intensity (elite: 108.9 ± 10.6 m · min-1; semi-elite: 102.3 ± 9.7 m · min-1), high-intensity exercise (elite: 36.7 ± 9.8 m · min-1; semi-elite: 29.6 ± 7.8 m min-1), mean playing speed (elite: 6.6 ± 0.6 km · h-1; semi-elite: 6.2 ± 0.6 km · h-1), and support play (elite: 0.29 ± 0.16 · min-1; semi-elite: 0.15 ± 0.09 · min-1) were all higher during first-half match-play in the elite than semi-elite players (P > 0.01). The elite players experienced decrements in most physical performance measures during the second-half of match-play (P > 0.01), which was not evident in the semi-elite players (P > 0.01). There were no differences in most physical performance and game-specific skill measures for the match between the two playing standards. These results show that while the two standards of competition have similar game-specific skills and physical demands during a match, there is variation within a match according to standard. Specifically, the higher physical demands placed on elite players during the first half could result in the earlier onset of physical fatigue towards the end of a match

Monitoring training loads in professional rugby league

N

Reflections of maternity service users and midwives on the co-creation of interventions to support midwives addressing alcohol during antenatal care

Background: There are divergent perspectives between midwives and pregnant women on how alcohol consumption during pregnancy could be addressed. Cocreation is an approach where lay people and professionals work together as equal partners, offering the opportunity to bridge the gap. Objectives: Our aim was to evaluate how well we carried out authentic co-creation of an intervention to support midwives have a dialogue about alcohol consumption with pregnant women. Patient involvement: Recent maternity service users including women with experience of harm due to alcohol during pregnancy provided feedback on the design, conduct and dissemination of the study. Methods: An iterative co-creation approach rooted in participatory research methods was used. Five online workshops were carried out with thirteen midwives and six maternity service users via Zoom July-August 2021. Data were analysed using the core values of co-create as a framework: equality, inclusivity, holistic, resource, positivity, transparency, iterative, and sustainability. Results: The co-creation process was productive and rewarding to midwives and maternity service users. There were positive experiences across the co-creation framework with some unintended positive consequences for maternity-service users. Discussion: This evaluation provides new knowledge on how well the co-creation process worked in relation to research involving a sensitive topic that can invite stigma. Co-creation projects require generous time and financial resources to ensure a high quality process and robust outcome for all. Practical value: Co-creation of strategies involving both service providers and service users have potential to facilitate evidence-based practice