17 research outputs found
Response of \u3cem\u3eEscherichia coli\u3c/em\u3e to Ferrous Ions. II. Influence of Nitrogen and Oxygen on the Mutagenic and Lethal effects of Fe++ for a Streptomycin-dependent Strain
Transformation of \u3cem\u3eNeisseria meningitidis\u3c/em\u3e by Deoxyribonucleates from Cells and from Culture Slime
Response of \u3cem\u3eEscherichia coli\u3c/em\u3e to Ferrous Ions. III. Application of a Method for Estimating Cellular Division to a Study of Recovery and Mutation
A Defined Agar Medium for Genetic Transformation of \u3cem\u3eNeisseria meningitidis\u3c/em\u3e
Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470–474. 1962.—An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine, sodium glutamate, and purified agar. Abundant surface growth in the absence of supplemental carbon dioxide was obtained during 50 serial subcultures. A close correspondence was found between numbers of parental type colonies developing on the defined medium and on a complex medium. Cells subcultured serially three or four times on defined agar medium and placed directly into a solution of transforming deoxyribonucleic acid in defined liquid medium were susceptible to transformation without additional supplements. Of the treated population, 0.1 to 0.3% of the cells were transformed to streptomycin resistance