Práticas profissionais no atendimento em casos de atribuição de apoios sociais

Compreendendo que o Trabalhador Social assume um papel importante no desenvolvimento da intervenção social, e visto ser o elo de ligação entre o cliente e a instituição, o presente trabalho pretende investigar o problema: Como é que as práticas profissionais do técnico no processo de atendimento concorrem para o desenvolvimento da intervenção? Rogers (1961), Cormier e Cormier (1998) e Egan (2003, citado por TACCA) referem que técnicas profissionais como escuta ativa, empatia, espontaneidade e aceitação incondicional promovem o estabelecimento da relação de ajuda. Segundo os mesmo autores, na relação estabelecida se o cliente se sentir ouvido, compreendido e respeitado envolve-se autonomamente no processo de intervenção social. A intervenção é considerada bem sucedida quando se verifica alteração ou transformação na situação trazida pelo cliente. Contudo, quando o técnico intervem, não pode descurar as normas institucionais e a legislação. Em conformidade com a questão enunciada e de forma a compreender os desafios, as dificuldades e as experiências dos profissionais, identificaram-se como objetivos desta investigação: identificar os maiores desafios experienciados pelo profissional nos atendimentos; conhecer as estratégias utilizadas para lidar com as dificuldades experienciadas no decurso do atendimento; compreender como o profissional estabelece os limites da relação de atendimento; identificar as circunstâncias em que o cliente manifesta hostilidade e, identificar estratégias de regulação em manifestações de hostilidade. A presente investigação carateriza-se por um estudo exploratório qualitativo. Os dados são recolhidos através de trinta e cinco observações naturalistas em contextos de atendimento a cinco profissionais da área social e, através de cinco entrevistas semi-estruturadas (aplicada uma por participante). Após a análise de conteúdo, constatou-se que os técnicos enumeraram fatores externos que podem influenciar o desenvolvimento do atendimento, como: ocultação de informações pessoais ou económicas pelo cliente, isolamento institucional e local, clientes com problemas psiquiátricos, incumprimento das regras pelos clientes e sobrecarga de trabalho imposto pela instituição. Verifica-se também que os profissionais, de acordo com o referido nas entrevistas, conhecem técnicas imprescindíveis ao estabelecimento de uma adequada relação profissional como a empatia, clarificação, sumarização, genuinidade e acolhimento caloroso. No entanto, quando realizadas as observações, são registadas inadequações no uso das técnicas ou ausência das mesmas. É, por exemplo, notória a ausência de recurso a técnicas subjacentes à realização de escuta ativa.Understanding that the Social Worker plays an important role in the development of social intervention, and since it is the link between the client and the institution, the present work intends to investigate the problem: How do the professional practices of the technician in the service process compete for the development of the intervention? Rogers (1961), Cormier and Cormier (1998) and Egan (2003, quoted by TACCA) point out that professional techniques such as active listening, empathy, spontaneity and unconditional acceptance promote the establishment of the aid relationship. According to the same authors, in the relation established if the client feels heard, understood and respected, it is autonomously involved in the process of social intervention. The intervention is considered successful when there is change or transformation in the situation brought by the client. However, when the technician intervenes, he can not neglect institutional norms and legislation. In accordance with the aforementioned question and in order to understand the challenges, the difficulties and the experiences of the professionals, the objectives of this research were identified as: to identify the greatest challenges experienced by the professionals in the care; to know the strategies used to deal with the difficulties experienced during the course of care; understand how the professional establishes the limits of the service relationship; identify the circumstances in which the client manifests hostility, and identify regulatory strategies in manifestations of hostility. The present investigation is characterized by a qualitative exploratory study. The data are collected through thirty-five naturalistic observations in contexts of service to five professionals in the social area and, through five semi-structured interviews (applied one per participant). After analyzing the contents, it was verified that the technicians enumerated external factors that could influence the development of the service, such as: hiding personal or economic information by the client, institutional and local isolation, clients with psychiatric problems, overload imposed by the institution. It is also verified that the professionals, according to the mentioned in the interviews, know techniques essential to the establishment of an adequate professional relation as empathy, clarification, summarization, genuineness and warm welcome. However, when observations are made, inadequacies in the use of the techniques or lack thereof are recorded. It is, for example, notorious for the lack of recourse to the techniques underlying the performance of active listening

The Effectiveness of Therapeutic Vaccines for the Treatment of Cervical Intraepithelial Neoplasia 3: A Systematic Review and Meta-Analysis

Cervical cancer (CC) is a disease that affects many women worldwide, especially in low-income countries. The human papilloma virus (HPV) is the main causative agent of this disease, with the E6 and E7 oncoproteins being responsible for the development and maintenance of transformed status. In addition, HPV is also responsible for the appearance of cervical intraepithelial neoplasia (CIN), a pre-neoplastic condition burdened by very high costs for its screening and therapy. So far, only prophylactic vaccines have been approved by regulatory agencies as a means of CC prevention. However, these vaccines cannot treat HPV-positive women. A search was conducted in several databases (PubMed, Scopus, Web of Science, and ClinicalTrials.gov) to systematically identify clinical trials involving therapeutic vaccines against CIN 3. Histopathological regression data, immunological parameters, safety, DNA clearance, and vaccine efficacy were considered from each selected study, and from the 102 articles found, 8 were selected based on the defined inclusion criteria. Histopathological regression from CIN 3 to CIN < 1 was 22.1% (95% CI: 0.627–0.967; p-value = 0.024), showing a vaccine efficacy of 23.6% (95% CI; 0.666–0.876; p-value < 0.001). DNA clearance was assessed, and the risk of persistent HPV DNA was 23.2% (95% CI: 0.667–0.885; p-value < 0.001). Regarding immunological parameters, immune responses by specific T-HPV cells were more likely in vaccinated women (95% CI: 1.245–9.162; p-value = 0.017). In short, these studies favored the vaccine group over the placebo group. This work indicated that therapeutic vaccines are efficient in the treatment of CIN 3, even after accounting for publication bias.info:eu-repo/semantics/publishedVersio

Large-kernel Attention for Efficient and Robust Brain Lesion Segmentation

Vision transformers are effective deep learning models for vision tasks, including medical image segmentation. However, they lack efficiency and translational invariance, unlike convolutional neural networks (CNNs). To model long-range interactions in 3D brain lesion segmentation, we propose an all-convolutional transformer block variant of the U-Net architecture. We demonstrate that our model provides the greatest compromise in three factors: performance competitive with the state-of-the-art; parameter efficiency of a CNN; and the favourable inductive biases of a transformer. Our public implementation is available at https://github.com/liamchalcroft/MDUNet

Root functioning, tree water use and hydraulic redistribution in Quercus suber trees: a modeling approach based on root sap flow

Mediterranean evergreen oaks have to survive a long summer drought. Roots may play a relevant role under these conditions. We studied their structure and function in a mature Quercus suber L. tree in central Portugal. The root system was mapped till the lowest water table level (4.5 m depth). Xylem anatomy was analyzed in a vertical profile belowground. Sap flow was continuously monitored for 1.5 yrs in the stem and roots of this intensively studied tree (heat field deformation method) and in the stem of four trees (Granier method), in relation to environmental variables and predawn leaf water potential. The sources of water uptake were assessed by stable isotope analyses in summer. Results showed a dimorphic root system with a network of superficial roots linked to sinker roots, and a taproot diverting into tangles of deep fine roots submerged for long periods, with parenchyma aerenchyma. Transpiration was not restricted in summer due to root access to groundwater. The isotopic d18O signature of twig xylem water was similar to that of groundwater in the dry season. Two functional types of superficial roots were identified: shallow connected and deep connected roots. A modeling approach was built considering that each superficial root was linked to a sinker, with part of the root deep connected (between the stem and the sinker) and part shallow connected (between the sinker and topsoil). This conceptual framework simulated tree stem sap flow from root sap flow with a high efficiency (R2 = 0.85) in four plot trees. On an annual basis, soil water and groundwater contributions were 69.5% and 30.5% of stem flow, respectively. Annual hydraulic lift and hydraulic descent were 0.9% and 37.0% of stem flow, respectively. The trees maximize the exploitation of the environmental resources by using the topsoil water during most of the year, and groundwater together with hydraulic lift (nutrient supply) in the dry summer. This study shows that a dimorphic root system, with roots reaching groundwater, is an efficient strategy of Q. suber trees to cope with seasonal drought. Knowledge of the functional behavior of Q. suber trees under shallow water table conditions may contribute to the definition of better adapted management practices and to anticipate their responses to climate chang

Drought reduces tree growing season lenght but increases nitrogen resorption efficiency in a Mediterranean ecosystem

Mediterranean ecosystems are hotspots for climate change, as the highest impacts are forecasted for the Mediterranean region, mainly by more frequent and intense severe droughts. Plant phenology is a good indicator of species’ responses to climate change. In this study, we compared the spring phenology of cork oak trees (Quercus suber), an evergreen species, over 2 contrasting years, a mild year (2004) and a dry year (2005), which was the most severe drought since records exist. We evaluated the timing of occurrence, duration, and intensity of bud development, budburst, shoot elongation, trunk growth, and leaf senescence (phenophases) and assessed the nitrogen resorption efficiency from senescent to green leaves. The temperature was the main driver of budburst. Nevertheless, water had the main role of constraining all the other phenophases by strongly reducing the growing season length (48 %) and consequently the tree growth. Basal area increment was the most affected growth variable (36 %), although it occurred at a similar rate in the 2 years. Shoot elongation was also reduced (21 %), yet elongation occurred at a higher rate in the dry year compared to the mild year. Leaf senescence during the bulk period was higher in the dry year, in which leaves were shed at the same rate over a longer period. Nitrogen concentrations in green and senescent leaves were affected by drought and nitrogen resorption efficiency increased remarkably (C22 %). Our results highlight the importance of studying different phenological metrics to improve our understanding of the ecosystem’s responses to climate change. The faster dynamics observed in shoot elongation, while all other phenophases developed at the same rate, indicate that leaf area development is privileged in cork oak. Water availability was the main driver of spring growth in this Mediterranean ecosystem; however, growth may be affected by complex interplays between precipitation and temperature, such as higher temperatures during dry winters or heatwaves during spring, that are likely to result in water stress. Longer studies are needed to disentangle those interplays. Finally, a higher nitrogen resorption efficiency in response to drought appears to be an adaptive trait that mitigates the limitation in nitrogen uptake by the roots during drought and contributes to improving tree fitness in the short term but will probably exert a negative feedback on the nitrogen cycle in the long term, which might affect the ecosystem functioning under the forecasted droughtsinfo:eu-repo/semantics/publishedVersio

Tropospheric methanol observations from space: retrieval evaluation and constraints on the seasonality of biogenic emissions

Methanol retrievals from nadir-viewing space-based sensors offer powerful new information for quantifying methanol emissions on a global scale. Here we apply an ensemble of aircraft observations over North America to evaluate new methanol measurements from the Tropospheric Emission Spectrometer (TES) on the Aura satellite, and combine the TES data with observations from the Infrared Atmospheric Sounding Interferometer (IASI) on the MetOp-A satellite to investigate the seasonality of methanol emissions from northern midlatitude ecosystems. Using the GEOS-Chem chemical transport model as an intercomparison platform, we find that the TES retrieval performs well when the degrees of freedom for signal (DOFS) are above 0.5, in which case the model : TES regressions are generally consistent with the model : aircraft comparisons. Including retrievals with DOFS below 0.5 degrades the comparisons, as these are excessively influenced by the a priori. The comparisons suggest DOFS > 0.5 as a minimum threshold for interpreting retrievals of trace gases with a weak tropospheric signal. We analyze one full year of satellite observations and find that GEOS-Chem, driven with MEGANv2.1 biogenic emissions, underestimates observed methanol concentrations throughout the midlatitudes in springtime, with the timing of the seasonal peak in model emissions 1-2 months too late. We attribute this discrepancy to an underestimate of emissions from new leaves in MEGAN, and apply the satellite data to better quantify the seasonal change in methanol emissions for midlatitude ecosystems. The derived parameters (relative emission factors of 11.0, 1.0, 0.05 and 8.6 for new, growing, mature, and old leaves, respectively, plus a leaf area index activity factor of 0.75 for expanding canopies with leaf area index < 2.0) provide a more realistic simulation of seasonal methanol concentrations in midlatitudes on the basis of IASI, TES, and ground-based measurements

GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use

<p>Abstract</p> <p>Background</p> <p>There is an increasing need for quantitative technologies suitable for molecular detection in a variety of settings for applications including food traceability and monitoring of genetically modified (GM) crops and their products through the food processing chain. Conventional molecular diagnostics utilising real-time polymerase chain reaction (RT-PCR) and fluorescence-based determination of amplification require temperature cycling and relatively complex optics. In contrast, isothermal amplification coupled to a bioluminescent output produced in real-time (BART) occurs at a constant temperature and only requires a simple light detection and integration device.</p> <p>Results</p> <p>Loop mediated isothermal amplification (LAMP) shows robustness to sample-derived inhibitors. Here we show the applicability of coupled LAMP and BART reactions (LAMP-BART) for determination of genetically modified (GM) maize target DNA at low levels of contamination (0.1-5.0% GM) using certified reference material, and compare this to RT-PCR. Results show that conventional DNA extraction methods developed for PCR may not be optimal for LAMP-BART quantification. Additionally, we demonstrate that LAMP is more tolerant to plant sample-derived inhibitors, and show this can be exploited to develop rapid extraction techniques suitable for simple field-based qualitative tests for GM status determination. We also assess the effect of total DNA assay load on LAMP-BART quantitation.</p> <p>Conclusions</p> <p>LAMP-BART is an effective and sensitive technique for GM detection with significant potential for quantification even at low levels of contamination and in samples derived from crops such as maize with a large genome size. The resilience of LAMP-BART to acidic polysaccharides makes it well suited to rapid sample preparation techniques and hence to both high throughput laboratory settings and to portable GM detection applications. The impact of the plant sample matrix and genome loading within a reaction must be controlled to ensure quantification at low target concentrations.</p

Overview of the interactive task in BioCreative V

Fully automated text mining (TM) systems promote efficient literature searching, retrieval, and review but are not sufficient to produce ready-to-consume curated documents. These systems are not meant to replace biocurators, but instead to assist them in one or more literature curation steps. To do so, the user interface is an important aspect that needs to be considered for tool adoption. The BioCreative Interactive task (IAT) is a track designed for exploring user-system interactions, promoting development of useful TM tools, and providing a communication channel between the biocuration and the TM communities. In BioCreative V, the IAT track followed a format similar to previous interactive tracks, where the utility and usability of TM tools, as well as the generation of use cases, have been the focal points. The proposed curation tasks are user-centric and formally evaluated by biocurators. In BioCreative V IAT, seven TM systems and 43 biocurators participated. Two levels of user participation were offered to broaden curator involvement and obtain more feedback on usability aspects. The full level participation involved training on the system, curation of a set of documents with and without TM assistance, tracking of time-on-task, and completion of a user survey. The partial level participation was designed to focus on usability aspects of the interface and not the performance per se. In this case, biocurators navigated the system by performing pre-designed tasks and then were asked whether they were able to achieve the task and the level of difficulty in completing the task. In this manuscript, we describe the development of the interactive task, from planning to execution and discuss major findings for the systems tested

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio