Total RNA was extracted from cucumber leaves at various time after spraying with water, 5 mM salicylic acid, 1 mM BTH, 50 mM L-α-amino butyric acid (ABA), 0

<p><b>Copyright information:</b></p><p>Taken from "Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment"</p><p>BMC Plant Biology 2004;4():15-15.</p><p>Published online 26 Aug 2004</p><p>PMCID:PMC516775.</p><p>Copyright © 2004 Bovie et al; licensee BioMed Central Ltd.</p>78 mM 2-thiouracil (2T-U) or from untreated leaves (UT). RNA (10 μg) was fractionated by electrophoresis on agarose gel. Northern blot was probed with α-P labeled PR-8 cDNA (loading of equal amounts of RNA was confirmed by ethidium bromide staining)

A: Total RNA was extracted from cucumber leaves at various time after application of 10 μM BTH or water (mock treatment)

<p><b>Copyright information:</b></p><p>Taken from "Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment"</p><p>BMC Plant Biology 2004;4():15-15.</p><p>Published online 26 Aug 2004</p><p>PMCID:PMC516775.</p><p>Copyright © 2004 Bovie et al; licensee BioMed Central Ltd.</p> RNA (15 μg) from leaves 1 and 2 (treated) and leaves 3 and 4 (untreated) was fractionated by electrophoresis on agarose gels. Identical Northern blots were probed with P-labeled PR-8, CPR and CGT cDNAs. B: Total RNA was extracted from cucumber leaves at various time after inoculation. RNA (15 μg) from untreated leaves (UT), leaves 1 (infected) and leaves 2, 3 and 4 (non infected) was fractionated by electrophoresis on agarose gels. Identical Northern blots were probed with P-labeled PR-8, CPR and CGT cDNAs. Equal loading of RNA was confirmed by ethidium bromide (Et Br) staining. A representative gel is shown

Quantitative assessment of DNA hypermethylation in the inflammatory and non-inflammatory breast cancer phenotypes

はじめに1.航空機生産の特徴2.エンジン生産メーカーの特徴 (以上,本号) 3.MRO ビジネス4.理論的考察おわり

Quantitative methylation profiling in tumor and matched morphologically normal tissues from breast cancer patients

Abstract Background In the present study, we determined the gene hypermethylation profiles of normal tissues adjacent to invasive breast carcinomas and investigated whether these are associated with the gene hypermethylation profiles of the corresponding primary breast tumors. Methods A quantitative methylation-specific PCR assay was used to analyze the DNA methylation status of 6 genes (DAPK, TWIST, HIN-1, RASSF1A, RARβ2 and APC) in 9 normal breast tissue samples from unaffected women and in 56 paired cancerous and normal tissue samples from breast cancer patients. Results Normal tissue adjacent to breast cancer displayed statistically significant differences to unrelated normal breast tissues regarding the aberrant methylation of the RASSF1A (P = 0.03), RARβ2 (P = 0.04) and APC (P = 0.04) genes. Although methylation ratios for all genes in normal tissues from cancer patients were significantly lower than in the cancerous tissue from the same patient (P ≤ 0.01), in general, a clear correlation was observed between methylation ratios measured in both tissue types for all genes tested (P RASSF1A. Notably, in 73% of patients, at least one gene with an identical methylation change in cancerous and normal breast tissues was observed. Conclusions Histologically normal breast tissues adjacent to breast tumors frequently exhibit methylation changes in multiple genes. These methylation changes may play a role in the earliest stages of the development of breast neoplasia.</p